Hsd3b7 (NM_133943) Mouse Tagged ORF Clone

CAT#: MR218387

Hsd3b7 (Myc-DDK-tagged) - Mouse hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hsd3b7
• Synonyms: AI195443; BB098564
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218387 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACTCTGCACAGGTGCCAACACTGGTATACCTGGTCACAGGTGGCTGCGGCTTCCTGGGGGAAC
  ATATTGTTCGGATGCTGCTGGAACGGGAGCCCAGGCTCCGGGAGCTGCGTGTCTTTGACCTGCACCTGAG
  TTCTTGGCTGGAGGAGCTGAAAGCAGGGCCTGTGCAGGTGACTGCCATCCAGGGGGATGTGACTCAGGCC
  CATGAGGTGGCAGCAGCCATGTCTGGATCACATGTGGTCATCCATACAGCTGGGTTGGTGGATGTGTTTG
  GGAAGGCCAGTCCAAAGACCATCCACAAAGTCAACGTGCAGGGCACACAGAATGTGATTGATGCTTGTGT
  GCAGACTGGCACTCAGTACCTGGTCTACACGAGCAGCATGGAAGTGGTGGGGCCTAACATCAAGGGCCAC
  CCCTTCTACAGGGGCAATGAAGATACCCCATATGAGGCAGTCCACAGCCATCCCTACCCATGCAGTAAAG
  CCCTTGCTGAGCAGCTGGTCCTCGAGGCCAATGGAAGGAAGGTCAATGGAGGGCTACCCCTGGTGACATG
  TGCCCTTCGACCCACGGGCATTTATGGTGAAGGTCATCAGGTCATGAGAGACTTCTACTACCAGGGACTG
  CGCTTTGGAGGTCGTCTATTTCGGGCCGTCCCAGCTTCTGTGGAGCACGGTCGGGTCTATGTTGGCAATG
  TTGCTTGGATGCACATACTGGTGGCCCGGGAGCTGGAGCAGCGGGCAGCACTCATGGGTGGCCAGGTGTA
  TTTCTGCTATGATAAGTCACCTTATAAAAGCTACGAGGACTTCAACATGGAGTTTCTGAGTCCCTGTGGT
  CTTCGACTGATAGGCGCCCACCCACTGCTGCCCTACTGGCTGCTAGTGCTGCTGGCTACCCTCAATGCCC
  TGCTGCAGTGGCTGCTCCGCCCACTGGTGCTGTACACACCCCTGCTGAATCCCTACACGCTGGCTATGGC
  CAACACCACCTTTACTGTCAGTACCAACAAGGCACAGCGGCATTTTGGCTACAAGCCCCTCTTCTCATGG
  GAAGAGAGCAGGACCCGCACCATTCAGTGGGTGCAGGCGATGGAGGGTTCAGCTCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218387 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADSAQVPTLVYLVTGGCGFLGEHIVRMLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQA
  HEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGH
  PFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGL
  RFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCG
  LRLIGAHPLLPYWLLVLLATLNALLQWLLRPLVLYTPLLNPYTLAMANTTFTVSTNKAQRHFGYKPLFSW
  EESRTRTIQWVQAMEGSAR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133943
• ORF Size: 1110 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133943.2, NP_598704.2
• RefSeq Size: 1823 bp
• RefSeq ORF: 1110 bp
• Locus ID: 101502
• UniProt ID: Q9EQC1
• Cytogenetics: 7 F3
• MW: 41.1 kDa
• Gene Summary: The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis (PubMed:11067870). Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953).[UniProtKB/Swiss-Prot Function]