Spag6l (NM_015773) Mouse Tagged ORF Clone

CAT#: MR218299

Spag6 (Myc-DDK-tagged) - Mouse sperm associated antigen 6 (Spag6)

Product Images

Circular map for MR218299

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spag6l
• Synonyms: PF16; Spag6; Spag6l1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218299 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAGCGGCAGGTGCTGCAAGTGTTCGAGCAGTACCAGAAAGCCCGGACGCAATTCGTGCAGATGG
  TGGCAGAGCTGGCGACAAGACCCCAAAACATCGAAACGCTGCAGAATGCAGGTGTAATGTCTTTGCTAAG
  GCCGCTTCTTCTGGATGTGGTCCCAACAATTCAACAGACTGCAGCTTTGGCTCTGGGGAGACTGGCCAAT
  TACAATGATGACTTAGCAGAAGCAGTCGTGAAAGGTGACATTCTTCCACAGCTTGTTTATTCATTGGCAG
  AACAAAATCGTTTTTACAAGAAGGCAGCTGCCTTTGTGTTGAGAGCAGTTGGGAAACACTCTCCTCAGCT
  GGCCCAGGCCATAGTTGACTGCGGAGCTCTGGATACACTGGTGATCTGCTTGGAGGATTTTGACCCTGGG
  GTCAAGGAGGCTGCAGCCTGGGCACTCGGATACATTGCAAGACACAACACAGAATTATCTCAAGCCGTGG
  TGGATGCAGGAGCAATTCCTCTTTTAGTCCTTTGTATCCAGGAGCCAGAAATTGCTTTGAAAAGGATCGC
  TGCCTCGGCCCTCAGTGATATCTCAAAGCACTCCCCAGAGTTAGCACAGACGGTGGTGGACGCAGGAGCT
  ATTGCTCACTTAGCCCAGATGATCCTCAACCCTGATGCAAAACTGAAGCGCCAGGTCCTCTCAGCTCTCA
  GTCAGATTGCAAAGCATTCTGTGGACTTGGCAGAGATGGTTGTTGAAGCAGAGATTTTCCCTGTTGTACT
  AACCTGCCTGAAGGACAAAGATGAATATGTGAAGAAAAATGCTTGCACCTTAATCAGAGAGATTGCAAAA
  CACACGCCTGAGCTCTCGCAGCTGATTGTTAATGCAGGAGGCGTGGCTGCCGTGATCGATTGCATTGGGT
  CCTGCAAAGGGAACATACGGCTGCCTGGCATCATGATGCTGGGTTACGTGGCTGCTCATTCTGAGAACCT
  GGCCATGGCGGTGATCATCTCCAAGGGTGTACCCCAGTTGTCAATCTGCCTGTCAGAAGAACCAGAAGAT
  CATATTAAGGCTGCTGCTGCCTGGGCCTTGGGGCAGCTGGGAAGACACACTCCAGAGCATGCCAGGGCTG
  TGGCTGTCACAAACACGCTGCCAGTCCTGCTGTCTTTGTACATGTCACCAGAGAGCTCTGAGGACCTACA
  GCTCAAAAGTAAAAAAGCCATAAAGAATATCCTTCAGAAATGCACCTACCTCCCAGCCCTTGAGCCGTTC
  CTGTACGACGCACCTCCCAATATCCTGAAACATGTTGTTGGGCAGTTCAGTAAGGTGCTGCCACATGACA
  GCAAAGCCCGGCGGCTTTTCGTGACTAGTGGTGGACTTAAAAAGGTTCAGGAGATAAAGGCAGAACCAGG
  CTCTCTCCTGCAGGAATACATCAACAGCATCAACAATTGTTACCCAGAGGAAATAGTAAGGTATTATTCA
  CCTGGATACTCCGATACACTTCTGCAGAGGGTGGACAGCTACCAACCACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218299 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMSLLRPLLLDVVPTIQQTAALALGRLAN
  YNDDLAEAVVKGDILPQLVYSLAEQNRFYKKAAAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPG
  VKEAAAWALGYIARHNTELSQAVVDAGAIPLLVLCIQEPEIALKRIAASALSDISKHSPELAQTVVDAGA
  IAHLAQMILNPDAKLKRQVLSALSQIAKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNACTLIREIAK
  HTPELSQLIVNAGGVAAVIDCIGSCKGNIRLPGIMMLGYVAAHSENLAMAVIISKGVPQLSICLSEEPED
  HIKAAAAWALGQLGRHTPEHARAVAVTNTLPVLLSLYMSPESSEDLQLKSKKAIKNILQKCTYLPALEPF
  LYDAPPNILKHVVGQFSKVLPHDSKARRLFVTSGGLKKVQEIKAEPGSLLQEYINSINNCYPEEIVRYYS
  PGYSDTLLQRVDSYQPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015773
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015773.2, NP_056588.1
• RefSeq Size: 2483 bp
• RefSeq ORF: 1524 bp
• Locus ID: 50525
• UniProt ID: Q9JLI7
• Cytogenetics: 16 A3
• MW: 55.3 kDa
• Gene Summary: Important for structural integrity of the central apparatus in the sperm tail and for flagellar motility.[UniProtKB/Swiss-Prot Function]