Kif9 (NM_010628) Mouse Tagged ORF Clone

CAT#: MR218252

Kif9 (Myc-DDK-tagged) - Mouse kinesin family member 9 (Kif9), transcript variant 2

Product Images

Circular map for MR218252

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kif9
• Synonyms: MGC141389
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218252 representing NM_010628
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTACTAGGAAAAAGGTTCAAGCGTTTGTTCGAGTCAGGCCTACTGATGACTTTGCTCATGAAATGA
  TCAAATATGGAGAAGACAACAAAAGCATCGACATCCACTTGAAGAAGGACACTCGGAGAGGGGTTGTCAA
  TAACCAGCAGACAGACTGGTCCTTCAAGCTGGATGGAGTGCTTCACAATGCCTCCCAGGACTTGGTTTAT
  GAAACAGTTGCCAAGGATGCGGTTTCTCAAGCCCTTGATGGGTACAATGGTACCATCATGTGTTACGGGC
  AGACAGGAGCCGGCAAGACATACACCATGACAGGGGCAACGGAGAATTACAAGCACCGCGGAATTCTCCC
  TCGTGCCCTGCAGCAGGTGTTCAGGATGATTGAAGAGCGTCCCACGCACGCCATCACAGTCCGTGTTTCC
  TACCTGGAAATCTACAATGAGAACCTGTTCGATCTCCTGTCCACTCTGCCCTACGTGGGACCCTCAGTCA
  CACCAATGACCATTGTGGAAAACCCTCAAGGGATCTTCATTAAAGGCTTGTCAGTGCATCTCACCAGTCA
  AGAGGAGGACGCCTTCAGCCTCCTCTTTGAGGGCGAGACCAACAGGATCATTGCCTCCCACACGATGAAC
  AAGAACTCATCCAGGTCGCACTGCATTTTCACCATCTACATGGAGGCCCACTCTCGGACCTTATCAGATG
  AAAAGTATATCACTTCTAAAATCAACCTGGTAGATCTGGCAGGCTCAGAGAGGCTCAGCAAGACTGGGTC
  TGAAGGTAGAGTCCTAAAGGAAGCCACCTACATCAACAAGTCACTGTCATTCCTGGAGCAGGCCATCATT
  GCCCTTGGGGACCAGAATCGGGACCACGTCCCTTTCCGGCAGAGCAAGCTCACCCATGCCCTGAAAGACT
  CCTTAGGGGGAAACTGTAATATGGTCCTCGTGACAAACATCTATGGAGAAGCTGCCCAGTTGGATGAAAC
  GCTGTCTTCATTGAGGTTTGCCAGCAGGATGAAGCTGGTCACCACTGAGCCTGCCATCAATGAGAAGTAT
  GATGCTGAGAGAATGGTCAAGAATTTGGAGAAGGAACTGGCATTGCTTAAGCAGGAACTGGCCATCCATG
  ATAGTCTGTCCAACCGCACCCTTGTGAATTATGACCCCATGGATGAAATTCAGATTGCTGAGATCAACTC
  TCAAGTACGGAGGTACCTGGAGGGGACGCTGGACGAGATTGACATAATCAATCTAAGGCAGATTCAGGAG
  GTATTCAACCAGTTCAGGGTGGTTCTGAGCCAACAGGAGCAGGAAGTAGAGTCTGCCTTGCGCAGGAAGT
  ACACTCTCATAGACAAAAATGATTTTGCAGCCATTTCTGCTGTCCAGAAGGTGGGGCTTATGGATATAGA
  AGGTAACCTAGTAGGTGAGCCCGATGGACAAAGCTTTGGACTTGGAGTTGCTCCGTTTTCTGTCAAACCT
  GGGAAGAAACCTAAGACCAAGAAGACACCGAAGGACCAGTTCAGCTCCTCAGCAAGAAAGGAAGGTGCCA
  GTAGCCCTGTGAGTGGCAAGGACTTTGATGTGGCTTCCATCTCCAAGACCCAGCTGATCCCATCTTCCAA
  AGATGGGGATCTCAAAGACATGCTTGCACGAGAGAGAGAAACTTCCAGCATTGAACCCCTTATCTCAGAC
  TCTCCAAAGGAAGAATTACGTGCGCCCAGACCCAGCACCCCACCCTCTAGGACTGTGGCCTTTGAAGAAT
  TTAAGAATGAACGAGGTAGTGAGATCAACCGCATCTTCAAAGAAAACAAATCCATCTTAAATGAACGGAA
  GAAAAGGGCCAGCGAGACAACCCAGCGCATCAACGCCATCAAGCAGGAGATTGATGAGACCAAAGATGCA
  CTCAATTTCCAGAAATCACTTCGGGAGAAGCAAGGAGAGTACGAGAACAAAGGCCTGATGATCATCGACG
  AGGAGGAATTTCTGTTGATCCTGAAGCTCAAAGACCTGAAGAAGCAGTATCGAAATGAGTACCAGGAACT
  TAGAGACCTCCGGGCTGAGATCCAATACTGCCAGCGCCTGGTGGATCAGTGTCGCCATCGCCTGCTCATG
  GAATTTGACATCTGGTACAATGAGTCTTTTATGATCCCAGAGGACGTTCAGGTAGCACTGAAGCTAGGCA
  GCAGCATCCGGCCAGGAATGGTGCCTATCAGCAGGATTGTGTGTCTGGGAGAAGATGACCAGGACAGGTT
  CAGCCATCTGCAGCAGACTGTCCTGCCTGAAGGTCTTGATTCCATCACCTTCTACAATGCCAAAGTCAAG
  ACAGATCAGAAGCACAATTATATGAAAACCATGGTGGGCCTCCAACAGTCACACAGAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218252 representing NM_010628
  Red=Cloning site Green=Tags(s)
  
  MGTRKKVQAFVRVRPTDDFAHEMIKYGEDNKSIDIHLKKDTRRGVVNNQQTDWSFKLDGVLHNASQDLVY
  ETVAKDAVSQALDGYNGTIMCYGQTGAGKTYTMTGATENYKHRGILPRALQQVFRMIEERPTHAITVRVS
  YLEIYNENLFDLLSTLPYVGPSVTPMTIVENPQGIFIKGLSVHLTSQEEDAFSLLFEGETNRIIASHTMN
  KNSSRSHCIFTIYMEAHSRTLSDEKYITSKINLVDLAGSERLSKTGSEGRVLKEATYINKSLSFLEQAII
  ALGDQNRDHVPFRQSKLTHALKDSLGGNCNMVLVTNIYGEAAQLDETLSSLRFASRMKLVTTEPAINEKY
  DAERMVKNLEKELALLKQELAIHDSLSNRTLVNYDPMDEIQIAEINSQVRRYLEGTLDEIDIINLRQIQE
  VFNQFRVVLSQQEQEVESALRRKYTLIDKNDFAAISAVQKVGLMDIEGNLVGEPDGQSFGLGVAPFSVKP
  GKKPKTKKTPKDQFSSSARKEGASSPVSGKDFDVASISKTQLIPSSKDGDLKDMLARERETSSIEPLISD
  SPKEELRAPRPSTPPSRTVAFEEFKNERGSEINRIFKENKSILNERKKRASETTQRINAIKQEIDETKDA
  LNFQKSLREKQGEYENKGLMIIDEEEFLLILKLKDLKKQYRNEYQELRDLRAEIQYCQRLVDQCRHRLLM
  EFDIWYNESFMIPEDVQVALKLGSSIRPGMVPISRIVCLGEDDQDRFSHLQQTVLPEGLDSITFYNAKVK
  TDQKHNYMKTMVGLQQSHRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010628
• ORF Size: 2370 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010628.3, NP_034758.2
• RefSeq Size: 2744 bp
• RefSeq ORF: 2373 bp
• Locus ID: 16578
• UniProt ID: Q9WV04
• Cytogenetics: 9 F2
• MW: 90.3 kDa