Sik2 (NM_178710) Mouse Tagged ORF Clone

CAT#: MR218168

  • TrueORF®

Sik2 (Myc-DDK-tagged) - Mouse salt inducible kinase 2 (Sik2)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_178710" in other vectors (4)


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Reconstitution Protocol

USD 852.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Sik2
Synonyms G630080D20Rik; Snf1lk2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR218168 representing NM_178710
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCATGGCGGATGGCCCGAGGCACTTGCAGCGCGGGCCAGTCCGGGTGGGGTTCTACGACATCGAGG
GCACGCTGGGCAAGGGCAACTTTGCCGTGGTGAAGCTGGGGCGGCACCGGATCACCAAGACGGAGGTGGC
TATAAAAATAATAGACAAGTCACAGCTGGATGCAGTAAACCTTGAGAAAATCTACCGAGAAGTACAGATA
ATGAAAATGCTCGACCATCCTCACATCATTAAACTGTATCAGGTAATGGAGACCAAAAGTATGTTGTACC
TTGTGACAGAATATGCCAAAAATGGAGAAATTTTTGATTATCTTGCTAATCATGGCCGATTAAATGAATC
TGAAGCCAGGAGAAAATTCTGGCAAATTCTGTCTGCTGTTGATTATTGCCATGGCCGGAAGGTAGTGCAC
AGAGACCTGAAGGCTGAAAATCTCCTGCTGGATAACAACATGAATATCAAAATAGCAGATTTCGGCTTTG
GAAATTTCTTTAAAACTGGTGAACTGCTGGCAACATGGTGTGGCAGCCCCCCTTATGCAGCCCCAGAAGT
CTTTGAAGGGCAGCAGTATGAAGGACCACAGCTGGATATATGGAGCATGGGAGTTGTTCTTTATGTCCTT
GTCTGTGGAGCTCTACCTTTTGATGGACCAACTCTCCCTATTTTGAGGCAGAGGGTTTTAGAAGGAAGAT
TCCGGATTCCTTATTTCATGTCAGAAGATTGTGAACACCTCATTAGAAGGATGTTGGTCCTAGATCCTTC
CAAACGGCTAAGCATAGCTCAAATCAAGGAGCACAAGTGGATGCTCATAGAGGTTCCTGTACAGAGGCCT
ATTCTCTACCCACAAGAACAGGAAAACGAGCCGTCCATTGGAGAATTTAATGAGCAGGTTCTTCGACTGA
TGCACAGCCTTGGGATTGATCAGCAGAAGACTGTTGAGTCTTTGCAAAACAAGAGCTATAATCACTTTGC
TGCCATTTATTTCTTGTTGGTGGAACGTCTAAAATCACATAGGAGCAGTTTTCCTGTGGAGCAGAGACTT
GATGGCCGCCAGCGTCGGCCTAGCACCATTGCTGAACAAACAGTTGCCAAGGCACAAACTGTGGGGCTGC
CAGTGACCTTGCATCCACCGAACGTGAGACTGATGCGATCTACCCTCCTCCCACAGGCATCCAATGTGGA
GGCCTTTTCATTTCCAACATCCAGCTGTCAAGCAGAAGCTGCCTTTATGGAGGAAGAGTGTGTCGACACT
CCAAAGGTGAATGGCTGCCTGCTTGACCCTGTGCCCCCTGTCCTGGTGAGGAAGGGATGCCAGTCACTGC
CCAGCAGTATGATGGAGACCTCCATTGATGAAGGCTTGGAGACAGAAGGAGAGGCTGAGGAAGACCCCAG
TCAGGCCTTTGAAGCTTTCCAGGCCACACGCAGTGGGCAGCGACGGCACACTCTGTCAGAAGTGACTAAC
CAATTGGTTGTGATGCCCGGGGCAGGGAAAATGTTCTCCATGAGTGATAACCCCTCCCTTGAGAGTGTGG
ACTCTGAGTATGATATGGGGTCTGCCCAGAGGGACCTGAACTTTCTGGAAGACAGCCCTTCCTTGAAGGA
CATCATGTTAGCCAATCAGCCGTCACCCCGCATGACATCTCCTTTCATAAGCCTCAGACCTGCCAACCCA
GCCATGCAGGCTCTGAGCTCACAGAAGCGTGAGGCCCACAACCGGTCCCCCGTGAGCTTCCGAGAAGGCC
GCAGAGCATCAGATACGTCCCTTACACAAGGAATTGTAGCATTTAGACAACATCTTCAGAATCTTGCTAG
AACCAAAGGAATTCTGGAGTTGAACAAAGTACAATTGCTGTATGAACAAATGGGATCAAACGCAGACCCT
ACCTTAACATCAACTGCTCCTCAGCTCCAAGACCTTTCGAGCAGTTGCCCTCAGGAGGAAATCTCCCAGC
AGCAGGAAAGTGTCTCCAGCCTGTCTGCCAGCATGCACCCTCAGCTCTCACCACAGCAAAGCTTGGAAAC
CCAGTACCTACAGCATCGACTCCAGAAGCCCAATCTTCTGCCAAAGGCCCAGAGTCCTTGTCCAGTGTAT
TGTAAAGAGCCACCTCGGAGCCTGGAACAGCAGCTACAGGAGCATAGGCTCCAACAGAAGCGACTCTTCC
TCCAGAAGCAGTCTCAGCTGCAAGCATATTTTAATCAGATGCAGATAGCAGAGAGCTCCTACCCTGGACC
AAGTCAGCAGCTGGCTCTTCCCCACCAGGAGACTCCACTGACATCCCAGCAGCCCCCATCATTCAGCCTG
ACCCAGGCCCTGAGTCCTGTCCTCGAGCCCTCTTCTGAGCAGATGCAATTTAGCTCTTTCCTCAGCCAAT
ACCCAGAGATGCAGTTGCAGCCACTGCCCTCTACTCCCGGCCCCCAGGCTCCACCTCCCTTACCCTCACA
ATTGCAGCAACATCAGCAGCCACCACCCCCACCACCACCCCCTCCACCACAGCAGCCAGGAGCTGCTCCA
ACCTCCTTACAGTTCTCCTATCAGACTTGTGAGCTGCCAAGCACCACTTCTTCTGTACCAAACTATCCTG
CTTCCTGTCACTATCCTGTGGATGGAGCCCAGCAGAGCAACCTCACAGGGGCGGACTGTCCCAGGAGCTC
AGGACTTCAGGACACCGCATCTAGCTATGACCCACTGGCCCTCTCTGAGCTCCCTGGACTCTTTGATTGT
GAAATGGTAGAAGCTGTGGATCCACAACACAATGGGGTTGTGAGCTGCTTAGCCCGGGAGACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR218168 representing NM_178710
Red=Cloning site Green=Tags(s)

MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREVQI
MKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKVVH
RDLKAENLLLDNNMNIKIADFGFGNFFKTGELLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVL
VCGALPFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLSIAQIKEHKWMLIEVPVQRP
ILYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTVESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRL
DGRQRRPSTIAEQTVAKAQTVGLPVTLHPPNVRLMRSTLLPQASNVEAFSFPTSSCQAEAAFMEEECVDT
PKVNGCLLDPVPPVLVRKGCQSLPSSMMETSIDEGLETEGEAEEDPSQAFEAFQATRSGQRRHTLSEVTN
QLVVMPGAGKMFSMSDNPSLESVDSEYDMGSAQRDLNFLEDSPSLKDIMLANQPSPRMTSPFISLRPANP
AMQALSSQKREAHNRSPVSFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQMGSNADP
TLTSTAPQLQDLSSSCPQEEISQQQESVSSLSASMHPQLSPQQSLETQYLQHRLQKPNLLPKAQSPCPVY
CKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPGPSQQLALPHQETPLTSQQPPSFSL
TQALSPVLEPSSEQMQFSSFLSQYPEMQLQPLPSTPGPQAPPPLPSQLQQHQQPPPPPPPPPPQQPGAAP
TSLQFSYQTCELPSTTSSVPNYPASCHYPVDGAQQSNLTGADCPRSSGLQDTASSYDPLALSELPGLFDC
EMVEAVDPQHNGVVSCLARET

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_178710
ORF Size 2793 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_178710.3, NP_848825.2
RefSeq Size 3561 bp
RefSeq ORF 2796 bp
Locus ID 235344
UniProt ID Q8CFH6
Cytogenetics 9 A5.3
MW 104.6 kDa
Gene Summary Phosphorylates 'Ser-789' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and inhibiting activity of TORCs, the CREB-specific coactivators, like CRTC2/TORC2 and CRTC3/TORC3 in response to cAMP signaling (PubMed:29211348).[UniProtKB/Swiss-Prot Function]

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