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Acer3 (NM_025408) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR218055L3
Lenti ORF clone of Acer3 (Myc-DDK-tagged) - Mouse alkaline ceramidase 3 (Acer3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $600.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Acer3
Synonyms 1110057L18Rik; 5430429L08Rik; AV015045; Phca
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR218055).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025408
ORF Size 801 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025408.2
RefSeq Size 4058 bp
RefSeq ORF 804 bp
Locus ID 66190
UniProt ID Q9D099
Cytogenetics 7 E1
Summary Endoplasmic reticulum and Golgi ceramidase that catalyzes the hydrolysis of unsaturated long-chain C18:1-, C20:1- and C20:4-ceramides, dihydroceramides and phytoceramides into sphingoid bases like sphingosine and free fatty acids at alkaline pH (PubMed:26474409). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:26474409). Controls the generation of sphingosine in erythrocytes, and thereby sphingosine-1-phosphate in plasma (By similarity). Through the regulation of ceramides and sphingosine-1-phosphate homeostasis in the brain may play a role in neurons survival and function (PubMed:26474409). By regulating the levels of proinflammatory ceramides in immune cells and tissues, may modulate the inflammatory response (PubMed:26938296).UniProtKB/Swiss-Prot Function
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Documents
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"NM_025408" in other vectors (4)
SKU Description Size Price
MC210295 Acer3 (untagged) - Mouse alkaline ceramidase 3 (Acer3), (10ug) 10 ug
$330.00
MG218055 Acer3 (tGFP-tagged) - Mouse alkaline ceramidase 3 (Acer3), (10ug) 10 ug
$500.00
MR218055 Acer3 (Myc-DDK-tagged) - Mouse alkaline ceramidase 3 (Acer3) 10 ug
$289.00 $300.00
MR218055L4 Lenti ORF clone of Acer3 (mGFP-tagged) - Mouse alkaline ceramidase 3 (Acer3) 10 ug
$600.00

Plasmid Map for MR218055L3

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218055L3
Circular map for MR218055L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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