Gfm2 (NM_001146043) Mouse Tagged ORF Clone

CAT#: MR218049

Gfm2 (Myc-DDK-tagged) - Mouse G elongation factor, mitochondrial 2 (Gfm2), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Circular map for MR218049

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gfm2
• Synonyms: 6530419G12Rik; A930009M04Rik; EF-G2mt; EFG2; MST027; RRF2mt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218049 representing NM_001146043
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACCAAATGGAGGATTTTTGCAGTGAATCATCAGAGAACGTTCAGTGTGCACCTTAATACCATGT
  GTTACTGCAAAATAAAAGCAAATTTAAAAAGATTAAAGACTCAGCTGCCTCTTACAAGAAACTACAGTTC
  TGCACCAGGCATAGCAGGAAGTGATGTCAAGTCTCTTCATTCCGTCATTAACCCTCCTGTGGCCAAAATT
  CGTAATATTGGAATTATGGCACATATTGATGCAGGCAAAACCACCACCACAGAGAGAATCCTGTACTATT
  CTGGATACACAAGATCATTGGGAGATGTCGATGATGGAGACACAGTGACGGATTTCATGGCTCAAGAGCG
  AGAAAGGGGCATTACCATCCAGTCAGCAGCTGTGACTCTCGACTGGAAAGGGTATAGAGTCAATCTGATT
  GATACTCCAGGTCACGTAGACTTCACTCTGGAGGTCGAGCGCTGCTTGAGGGTGTTGGACGGTGCGGTGG
  CCGTGTTTGACGCCTCTGCTGGTGTAGAGGCCCAGACTCTCACTGTATGGAGGCAAGCTGACAAACACAA
  AATACCCCGGATCTGCTTTTTAAACAAGATGGACAAGACTGGAGCAAGTTTTAACTATGCGGTTGAAAGC
  ATCCGGGAGAAGTTAAAGGCAAAGCCCCTGATTTTACAGCTGCCAATTGGTGAAGCCAGAACTTTCCAAG
  GAGTGGTGGATGTCGTAAACAAAGAAAAACTCCTTTGGAATTCTAACTCTGACGATGGAAAAGACTTCGA
  GAGGATGCCCCTCTCAGAAGCGAGTGATCGGGAACTGCTAAAGGAGACAATTGAAGCAAGGAACTCCTTA
  ATTGAACAAGTTGCAGACTTAGATGATGAGTTTGCTGACTTGGTTTTAGGTGAATTTAGTGAAAACTTTG
  ATTTAGTACCAGCTGAAAAGTTACAGGCTGCAGTACACAGGGTGACACTGGCACAGGCTGCAGTGCCTGT
  GCTGTGTGGGAGTGCCTTGAAGAACAAGGGCGTGCAGCCCCTGCTGGATGCGGTGACCACTTACCTGCCG
  TCACCCGAGGAGCGCGAGGACCGCTTTCTGCAGTGGTATGAGGGTGACTTGTGTGCACTAGCGTTTAAAG
  TACTACATGACAAACAGCGAGGACCCCTGGTTTTCCTGCGCATTTACTCGGGAACACTGACGCCTCAGCT
  GGCTGTCCACAACATTAATAGAAACTGCACGGAAAGAATGAGTCGTCTGCTTTTGCCATTTGCTGACCAA
  CATGTAGAAATCCCTTCCCTGACTGCTGGAAACATTGCTTTGACAGTTGGGCTTAAACAAACTGCCACTG
  GAGACACCATAGTCTCGTCCAAGTCCAGCGCATTAGCTGCAGCTCGCAGAGCCGGACGAGGAGAAAGGGA
  GCATGGAAAGAAGAGAGAGGCCGAGAGTCTGCTGTTGGCCGGGGTGGAGGTCCCAGAGCCTGTCTTCTTC
  TGCACCATAGAGCCCCCGTCAGTCGCTAAGCAGCCAGATTTAGATCATGCCTTGGAACGCCTTCAGCGCG
  AAGATCCCAGTTTGAAAGTGAAACTCGATCCTGACTCTGGACAAACTGTCCTGTGTGGAATGGGGGAGTT
  GCATATTGAGATTATTCATGACCGTATCAAGAGGGAGTATGGCCTAGAGACCTATCTAGGGCCTCTGCAG
  GTGGCATACCGGGAAACCATCTTAAACTCAGTTCGCGCCACAGACACCCTCGATAGGGTGCTTGGAGACA
  AGCGGCACTTGGTGAGTGCGGAGCTGGAAGTCCGGCCAGCAGAAGAGCCCTGTGCTGTGGCCAAGATCGA
  GTATGCTGACTGTGTGGGTGAAGACCTGCTGCAGGCCTCTCGGGAGGCCATTGAAAGTGCCGTCCACAGT
  GCATGCCTCCAGGGACCTCTGCTCGGATCCCCAGTCCAGGATGTGGCAATGACGTTGCACTCACTGATGA
  TTCACCCTGGCACCTCCACAACTATGGTCACTGCCTGCATCTCACGGTGTATGCAGAAGGCCCTGAAGAA
  AGCTGACAAACAGGTGCTGGAACCTCTGATGAGTCTCGAGGTCACAGTGAGCAGGGAGTACCTCAGCCCT
  GTCCTGGCAGACTTGGCACAAAGAAGAGGGAACATTCAGGAAATCCAGACTCGCCAAGACAACAGAGTTG
  TTCTAGGATTCGTTCCTCTAGCAGAGATTATGTCCCTTCAGCTGGCTGATGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218049 representing NM_001146043
  Red=Cloning site Green=Tags(s)
  
  MFTKWRIFAVNHQRTFSVHLNTMCYCKIKANLKRLKTQLPLTRNYSSAPGIAGSDVKSLHSVINPPVAKI
  RNIGIMAHIDAGKTTTTERILYYSGYTRSLGDVDDGDTVTDFMAQERERGITIQSAAVTLDWKGYRVNLI
  DTPGHVDFTLEVERCLRVLDGAVAVFDASAGVEAQTLTVWRQADKHKIPRICFLNKMDKTGASFNYAVES
  IREKLKAKPLILQLPIGEARTFQGVVDVVNKEKLLWNSNSDDGKDFERMPLSEASDRELLKETIEARNSL
  IEQVADLDDEFADLVLGEFSENFDLVPAEKLQAAVHRVTLAQAAVPVLCGSALKNKGVQPLLDAVTTYLP
  SPEEREDRFLQWYEGDLCALAFKVLHDKQRGPLVFLRIYSGTLTPQLAVHNINRNCTERMSRLLLPFADQ
  HVEIPSLTAGNIALTVGLKQTATGDTIVSSKSSALAAARRAGRGEREHGKKREAESLLLAGVEVPEPVFF
  CTIEPPSVAKQPDLDHALERLQREDPSLKVKLDPDSGQTVLCGMGELHIEIIHDRIKREYGLETYLGPLQ
  VAYRETILNSVRATDTLDRVLGDKRHLVSAELEVRPAEEPCAVAKIEYADCVGEDLLQASREAIESAVHS
  ACLQGPLLGSPVQDVAMTLHSLMIHPGTSTTMVTACISRCMQKALKKADKQVLEPLMSLEVTVSREYLSP
  VLADLAQRRGNIQEIQTRQDNRVVLGFVPLAEIMSLQLADG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146043
• ORF Size: 2223 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146043.2, NP_001139515.1
• RefSeq Size: 4482 bp
• RefSeq ORF: 2226 bp
• Locus ID: 320806
• UniProt ID: Q8R2Q4
• Cytogenetics: 13 D1
• MW: 82.5 kDa
• Gene Summary: Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.[UniProtKB/Swiss-Prot Function]