Puf60 (NM_028364) Mouse Tagged ORF Clone

CAT#: MR218047

Puf60 (Myc-DDK-tagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 1

Product Images

Circular map for MR218047

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Puf60
• Synonyms: 2410104I19Rik; 2810454F19Rik; SIAHBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218047 representing NM_028364
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGGCGACTATAGCTCTCCAGGTCAATGGCCAACAAGGAGGAGGGTCGGAGCCAGCAGCAGCGG
  CTGCAGCGGCGGCGGCGGCAGTGGTGGCAGCAGGAGACAAATGGAAACCTCCACAGGGCACAGAATCCAT
  CAAGATGGAAAATGGACAAAGCACAGGCACCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAG
  GCCCTCCAGAAGGCCAAGAAATATGCAATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAACAGACCATCG
  CGCACCAGCAGCAGCAGCTCACCAACCTGCAGATGGCAGCAGTGACAATGGGCTTTGGAGATCCTCTCTC
  ACCTTTGCAATCGATGGCAGCTCAACGGCAGCGGGCACTGGCTATCATGTGCCGGGTCTATGTGGGCTCC
  ATCTACTATGAGCTGGGAGAAGACACTATTCGCCAGGCCTTTGCTCCCTTTGGCCCCATCAAGAGCATTG
  ATATGTCCTGGGACTCCGTTACCATGAAGCATAAGGGCTTTGCCTTCGTGGAGTACGAGGTCCCAGAAGC
  TGCGCAGCTCGCTTTGGAGCAGATGAACTCTGTGATGCTTGGGGGCAGGAACATCAAGGTGGGAAGACCC
  AGCAACATTGGACAAGCCCAACCCATCATAGATCAGCTGGCTGAGGAAGCTAGGGCTTTCAACCGAATAT
  ATGTGGCCTCTGTACATCAAGACCTGTCTGATGATGACATCAAGAGTGTATTTGAAGCCTTTGGCAAGAT
  CAAGTCTTGTACGCTGGCCCGGGACCCCACAACTGGCAAGCACAAGGGCTATGGTTTTATCGAATATGAG
  AAGGCCCAGTCGTCCCAGGATGCTGTGTCCTCCATGAACCTCTTTGATCTGGGTGGCCAGTACTTGAGGG
  TGGGCAAGGCCGTCACACCCCCCATGCCCCTGCTAACACCTGCCACGCCTGGAGGTCTCCCGCCTGCTGC
  TGCTGTGGCCGCAGCTGCAGCCACGGCCAAGATTACAGCTCAGGAAGCAGTGGCTGGAGCAGCAGTGCTG
  GGTACTTTAGCCACACCAGGACTAGTGTCCCCAGCACTGACACTGGCCCAGCCCTTAGGGGCCCTACCCC
  AGGCTGTCATGGCTGCCCAGGCCCCTGGAGTCATCACAGGTGTGACACCAGCCCGCCCTCCTATTCCGGT
  CACCATCCCCTCTGTGGGAGTAGTGAACCCCATTCTCGCCAGCCCGCCAACGTTGGGTCTGTTGGAGCCC
  AAGAAGGAGAAGGAAGAGGAGGAGCTGTTTCCTGAGTCAGAACGGCCAGAGATGTTGAGTGAGCAGGAGC
  ACATGAGCATCTCGGGCAGCAGTGCTCGCCACATGGTCATGCAAAAGCTACTCAGAAAACAGGAGTCTAC
  AGTGATGGTTCTCCGAAACATGGTGGACCCCAAGGACATCGATGACGACCTGGAGGGAGAGGTGACAGAG
  GAGTGTGGCAAATTTGGTGCTGTGAACCGGGTCATTATCTATCAAGAAAAACAGGGCGAAGAGGAAGATG
  CAGAAATCATCGTCAAGATTTTTGTGGAATTCTCCATGGCCTCAGAGACTCACAAGGCCATCCAGGCCCT
  CAATGGGCGCTGGTTTGGTGGTCGCAAGGTGGTGGCTGAAGTGTATGACCAGGAGCGTTTTGATAACAGC
  GACCTCTCCGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218047 representing NM_028364
  Red=Cloning site Green=Tags(s)
  
  MATATIALQVNGQQGGGSEPAAAAAAAAAAVVAAGDKWKPPQGTESIKMENGQSTGTKLGLPPLTPEQQE
  ALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGS
  IYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRP
  SNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYE
  KAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVL
  GTLATPGLVSPALTLAQPLGALPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEP
  KKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTE
  ECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMASETHKAIQALNGRWFGGRKVVAEVYDQERFDNS
  DLSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028364
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028364.2, NP_082640.2
• RefSeq Size: 1929 bp
• RefSeq ORF: 1695 bp
• Locus ID: 67959
• UniProt ID: Q3UEB3
• Cytogenetics: 15 D3
• MW: 60.7 kDa
• Gene Summary: DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA (By similarity).[UniProtKB/Swiss-Prot Function]