Slc39a8 (NM_026228) Mouse Tagged ORF Clone

CAT#: MR217622

Slc39a8 (Myc-DDK-tagged) - Mouse solute carrier family 39 (metal ion transporter), member 8 (Slc39a8), transcript variant 3

Product Images

Circular map for MR217622

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc39a8
• Synonyms: 4933419D20Rik; AA986696; BIGM103; ZIP-8; Zip8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217622 representing NM_026228
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCGGGCCGGGCAGTGGCCGGGCTGCTGCTGCTGGCGGCCACCAGCCTGGGACACCCCTCGGAGG
  GACCGGAGCTCGCCTTCAGTGAGGATGTGCTGAGCGTGTTCGGCGCCAACCGGAGCCTGTCGGCCGCGCA
  GCTGGGGCGCCTGCTGGAGCGTCTGGGGGCTGCATCCCAGCAGGGAGCGCTGGACCTGGGCCAGCTGCAC
  TTCAACCAGTGTTTGTCAGCAGAAGACATCTTTTCTCTTCATGGTTTCTCAAATGTCACCCAGATAACCA
  GCTCGAACTTCTCTGCCATCTGCCCCGCGATCTTACAGCAGCTGAACTTCCATCCCTGCGAGGATCTAAG
  AAAGCACAACGCAAAGCCCAGTCTTTCCGAAGTCTGGGGCTATGGATTCCTGTCAGTGACAATTATCAAT
  CTGGCCTCTCTCCTGGGATTGATTTTAACCCCCTTGATAAAGAAGTCTTATTTCCCCAAGATTTTAACTT
  ATTTCGTGGGACTCGCTATTGGGACTCTTTTCTCAAACGCCATTTTCCAGCTTATTCCAGAGGCATTTGG
  ATTTAATCCCAAAATTGACAATTACGTTGAGAAAGCAGTTGCTGTGTTTGGTGGATTTTACATGCTTTTC
  TTTGTCGAGAGAACACTTAAGATGCTACTGAAAACATATGGCCAGAATGACCATACTCACTTCAGGAATG
  ATGACTTTGGTTCTAAAGAAAAAACCCACCAACCCAAAACGTTACCATTGCCTGCAGTCAACGGTGTGAC
  TTGCTATGCCAACCCCGCTGTCACTGAGCCTAACGGACACATCCACTTCGACACTGTCAGCGTTGTATCC
  CTCCAGGATGGAAAAACAGAGCCAAGCTCATGTACCTGTCTGAAGGGGCCCAAACTGTCAGAAATAGGAA
  CAATTGCCTGGATGATCACGCTTTGTGATGCCCTCCACAATTTCATCGACGGCTTGGCGATTGGGGCATC
  TTGTACTTTGTCTCTTCTTCAGGGGCTCAGTACGTCCATAGCGATCCTGTGTGAGGAGTTTCCTCATGAG
  TTAGGGGACTTTGTGATCTTGCTCAATGCAGGAATGAGCACCCGGCAAGCCTTGCTGTTCAACTTCCTCT
  CCGCGTGTTCCTGCTACGTGGGACTAGCTTTCGGCATTTTGGTGGGCAACAATTTTGCTCCCAATATTAT
  ATTTGCACTCGCTGGAGGCATGTTCCTCTACATTTCTCTGGCAGATATGTTTCCAGAGATGAACGACATG
  CTGAGAGAAAAAGTAACTGGAAGACAAACGGATTTCACCTTCTTCATGATCCAGAATGCAGGGATGTTAA
  CCGGCTTCACTGCCATCCTGCTCATCACCTTGTACGCAGGAGACATCGAATTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217622 representing NM_026228
  Red=Cloning site Green=Tags(s)
  
  MAPGRAVAGLLLLAATSLGHPSEGPELAFSEDVLSVFGANRSLSAAQLGRLLERLGAASQQGALDLGQLH
  FNQCLSAEDIFSLHGFSNVTQITSSNFSAICPAILQQLNFHPCEDLRKHNAKPSLSEVWGYGFLSVTIIN
  LASLLGLILTPLIKKSYFPKILTYFVGLAIGTLFSNAIFQLIPEAFGFNPKIDNYVEKAVAVFGGFYMLF
  FVERTLKMLLKTYGQNDHTHFRNDDFGSKEKTHQPKTLPLPAVNGVTCYANPAVTEPNGHIHFDTVSVVS
  LQDGKTEPSSCTCLKGPKLSEIGTIAWMITLCDALHNFIDGLAIGASCTLSLLQGLSTSIAILCEEFPHE
  LGDFVILLNAGMSTRQALLFNFLSACSCYVGLAFGILVGNNFAPNIIFALAGGMFLYISLADMFPEMNDM
  LREKVTGRQTDFTFFMIQNAGMLTGFTAILLITLYAGDIELQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026228
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026228.5, NP_080504.3
• RefSeq Size: 3492 bp
• RefSeq ORF: 1389 bp
• Locus ID: 67547
• UniProt ID: Q91W10
• Cytogenetics: 3 63.04 cM
• MW: 50.5 kDa
• Gene Summary: Acts as a manganese and zinc influx transporter. Plays a role in manganese reabsorption in the proximal tubule of the kidney and in manganese uptake into the brain.[UniProtKB/Swiss-Prot Function]