Kctd1 (NM_001142731) Mouse Tagged ORF Clone

CAT#: MR217534

Kctd1 (Myc-DDK-tagged) - Mouse potassium channel tetramerisation domain containing 1 (Kctd1), transcript variant 1

Product Images

Circular map for MR217534

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kctd1
• Synonyms: 4933402K10Rik; AI661543; AW553000
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217534 representing NM_001142731
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGAATGCCTGGCAGCGGGGACTGTAACACCAGCGCGGGCGGCAGCGCCGCTGCTGCGGCCGAGA
  ACAATGGGGATCGGGGTGAGGGTGAGCGCGGGGCCGGGGGCCGCGGCCGCCGCTACGGCCGCCCGCATTA
  CTGCAGCGCGGGCGAGGAGGAGGAGGAGGAAGAGGAGGAGGACGAGATCCAGGAGGTGCAGATAACGGGG
  GACGAGGAGGAGGACGGAGGTGGGGGGCTGGAGGAGGACGAGGAGGAGGAGGAAGAGGAGGAGATGGGGA
  TGGACTGGGAAGAGCCCCTGGAGCCGGAGGACTCGGCCGGGGAGGAGCTGGAGCCGGAGCCGGTCCATAT
  GATCCATATGGACCAGAGCGCCGCGCTGGAGCCCGAGGCGCCGCCACGACTGTTGGCGCCCCGGGCCCGC
  GCGGGGCCGCCGGGGGACAGCGCGGAGCTGGACCCCGACGTGCTGCAGCGCCCCGAGCGGGCCCGGCTGA
  GCGAGAACACCAGGCTGGCCACCCGCTACGCCGTGCGCATCTTCCGGGAGTACCTGAGCGAAAAGGCTCA
  GAGCCCGGACTTCGAGACCATGGACAAGGGGGCGCTGTGCCGCGTGCTGCGCTCCTTCTATGCCGAGGCC
  CGTTCTAAGAGCGGCCAGCTCTACAGCAAGTCCTCGCTCATCAGTATCCGCAGCTCCCTCAACCGCTACC
  TCAACGAGCCCCCTTACTGTCGCACGCTCGACCTCACCAAGGACCCCGAGCTGCGCAGCGCCAACCTGAC
  GCTGGCCGCAGTCATCCGCAAGCTCGAGGAGCAGGGCGCGGGGCCCGTGGTGCAGAAGCAAGCCATCACG
  CGCGCCGACCTGCGCAAGCTCTACACCTCCAGCGTCTTCAGCACCAACACGCCCTTTGGGCTGCTCAACA
  AGGTCTGGTTCGAGACGTGCATGTACTTCTGCACGCGAGGCCGTGAGAACCAGCGCGAGCTGGAGGAGGA
  CTCCTTCGGGCTGGCCATGGACGAGGACGGTCGCAAGTTCGTTTACTTCAAGTCGCTTGGGCCTTACCAT
  AAGTCGCGTTCCTCGTCCTGGAGTAAGAAGCGTGCGGAGAGCAGCGACGAAGAGAACTTGCCTCGCATGT
  ACGAGACGGGCACTGAGTTCTGTCCCTACGCCAGCTTTGTCAAGTACCTGTCTAAGCGCAATCCCCTCTG
  TAAGGCGTTTTTCCAGCGGCCCCGGGACCACTGCAGTGAGGGCGATGTGACATGGTACGAGAACAAAGCC
  ATAGGCAAGAACTTGCTGGGCACCAGGATGCAGATGCTGTCCAAGGCAGCCAAGCTGTCCAAGACCTACA
  CTAACCATTGCATCGGCGCGGTGTCCATCGCCACACTCAACAGCATCGCGGGTATCGGGACCAAGCTAGG
  CTCCCCAGCCCCGCAGGGCTGCTATACCGACTCTCTGAACGGGTCAGCTCGTCACCACTCCCACCACGCT
  CCCACCCATCCTTCCCACCACCACCGCCCCCAGCCGCCCTCGCTGGGTAACACTTATATCCTCCCCAAAG
  ACAGCCAGGTCGGGCCCGACGTGAAGTCCGAGGCGGCGCCCAAGCGCACGCTGTACGAGTCAGTGTTCGG
  GTCAGGGGAAATCTGCGGCCCTTCTTCCCCCAAAAGACTTTGTATCCGTCCCTCCTCGGAACCTGTGGAT
  GCGGTGGTGGTGGTTTCCGTGAAACACGACCCCCTGCCTCTTCTTCCAGAAGTCAATGGGCACCGAAGCA
  CCAATTCTCCCACCATAGTTTCACCTGCTATTGTTTCCCCCGCCCAGGACAGTCGGCCCAATATGTCAAG
  ACCTCTGATCACTCGATCCCCTGCATCGCCACTGAACAACCAAGGCATTCCAACTCCAGCTCAGCTCACC
  AAATCCAACGCACCCGTCCACATCGACGTGGGCGGCCACATGTACACCAGCAGCCTGGCCACCCTCACCA
  AATACCCCGAATCCAGAATCGGAAGACTCTTTGATGGTACAGAGCCCATTGTTCTGGACAGTCTCAAACA
  GCACTATTTCATTGACAGAGACGGGCAGATGTTCAGATATATCTTGAATTTTCTACGAACATCAAAACTC
  CTCATTCCTGATGATTTCAAGGACTATACTTTGTTATATGAAGAGGCAAAATACTTTCAACTTCAGCCAA
  TGTTGTTGGAGATGGAGCGATGGAAACAGGACAGGGAAACTGGCCGCTTTTCACGGCCCTGCGAGTGCCT
  CGTGGTGCGTGTGGCCCCGGACCTTGGAGAAAGGATCACCCTCAGTGGGGACAAGTCCTTGATAGAGGAA
  GTATTTCCCGAGATCGGCGATGTGATGTGCAACTCGGTCAATGCAGGCTGGAATCACGACTCAACACATG
  TCATCAGGTTTCCACTAAACGGCTACTGCCACCTCAATTCAGTTCAGGTCCTTGAGCGGTTGCAGCAAAG
  AGGATTTGAAATTGTGGGTTCCTGTGGAGGAGGAGTGGACTCGTCCCAGTTCAGTGAATATGTCCTCCGA
  CGGGAACTGAGGCGGACACCCCGAGTACCCTCAGTCATCCGGATAAAACAGGAGCCACTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217534 representing NM_001142731
  Red=Cloning site Green=Tags(s)
  
  MARMPGSGDCNTSAGGSAAAAAENNGDRGEGERGAGGRGRRYGRPHYCSAGEEEEEEEEEDEIQEVQITG
  DEEEDGGGGLEEDEEEEEEEEMGMDWEEPLEPEDSAGEELEPEPVHMIHMDQSAALEPEAPPRLLAPRAR
  AGPPGDSAELDPDVLQRPERARLSENTRLATRYAVRIFREYLSEKAQSPDFETMDKGALCRVLRSFYAEA
  RSKSGQLYSKSSLISIRSSLNRYLNEPPYCRTLDLTKDPELRSANLTLAAVIRKLEEQGAGPVVQKQAIT
  RADLRKLYTSSVFSTNTPFGLLNKVWFETCMYFCTRGRENQRELEEDSFGLAMDEDGRKFVYFKSLGPYH
  KSRSSSWSKKRAESSDEENLPRMYETGTEFCPYASFVKYLSKRNPLCKAFFQRPRDHCSEGDVTWYENKA
  IGKNLLGTRMQMLSKAAKLSKTYTNHCIGAVSIATLNSIAGIGTKLGSPAPQGCYTDSLNGSARHHSHHA
  PTHPSHHHRPQPPSLGNTYILPKDSQVGPDVKSEAAPKRTLYESVFGSGEICGPSSPKRLCIRPSSEPVD
  AVVVVSVKHDPLPLLPEVNGHRSTNSPTIVSPAIVSPAQDSRPNMSRPLITRSPASPLNNQGIPTPAQLT
  KSNAPVHIDVGGHMYTSSLATLTKYPESRIGRLFDGTEPIVLDSLKQHYFIDRDGQMFRYILNFLRTSKL
  LIPDDFKDYTLLYEEAKYFQLQPMLLEMERWKQDRETGRFSRPCECLVVRVAPDLGERITLSGDKSLIEE
  VFPEIGDVMCNSVNAGWNHDSTHVIRFPLNGYCHLNSVQVLERLQQRGFEIVGSCGGGVDSSQFSEYVLR
  RELRRTPRVPSVIRIKQEPLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142731
• ORF Size: 2583 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142731.1, NP_001136203.1
• RefSeq Size: 3456 bp
• RefSeq ORF: 2586 bp
• Locus ID: 106931
• Cytogenetics: 18 A1
• MW: 96.1 kDa
• Gene Summary: May repress the transcriptional activity of AP-2 family members, including TFAP2A, TFAP2B and TFAP2C to various extent.[UniProtKB/Swiss-Prot Function]