Aven (NM_028844) Mouse Tagged ORF Clone

CAT#: MR217465

Aven (Myc-DDK-tagged) - Mouse apoptosis, caspase activation inhibitor (Aven), transcript variant 1

Product Images

Circular map for MR217465

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aven
• Synonyms: 1700013A01Rik; 1700056A21Rik; mAven-L; mAven-S
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217465 representing NM_028844
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCTGAGCGCGGGGCTAGGGGCGGCCGCGGGCGGCGGGGAGGCCGGGAGCGGCCCGGAGGGGACC
  GAGAGCCGGTCGGGGCAGCGACGGCGCTGGCGAGAGGAGGCTGCGGGGACGGAGGCGGCCGGCGGGGCCG
  AGGCCGGGGCTTCCGCCGAGGCCGAGGAGGTGGCGGCCTGCGAGGCGGCCGCTGGGAGCCTGGAGGCCGG
  GGCGGCGGAGCCAGCACTCGGGTGGAAGAAGACAGCGATTCAGAGACCTATGGAGAAGAGAATGATGAGC
  AAGGAAATTTTTCTAGAAGAAAGATTGTCTCCAACTGGGATCGCTATCAAGATACTGAAAAGGAGGTCAA
  TGGTGAAAGTGGAGAATCTCAGCGGGGCACAGACTTCAGTGTCCTCCTGAGCTCTGCAGGGGACTCCTTT
  TCACAGTTCCGATTTGCTGAGGAGAAAGAATGGGATGGTGAAACTTCATGTCCAAAACAGAATTCAGCAC
  TCTACGTGGACAGTGAGTCACTGGTTCGAGCCCTTGAGCAGCTGCCTCTTGCAGTCCGGCTTAATGTTGC
  TTCAGAATTGATCCAGACCACAATTCCTTTAGAACTTCCACAGGTGAAACCAAGGAGAAACGATGATGGC
  AAGGAGCTGGGCATGCATTTAAGGGGACCCATCTCTGAGCTCAGATCTGCTGCTGGTGCTTGCCCCAGGT
  CTCTGGGCAGAGGCAGTCTAAGGCAAAGCCCTTTAGAAGGTTTGCAGAAAGCACCTACCCCAACACAGTC
  AGTGGCAGACCACCTGGAAGAAGAACTAGATATGTTGCTGCATTTAGATGCACCTGTGCAAGAGGAAGGC
  AATATCTCTCCAGACCAGACATCTCGGGACCAGGAACCAGAAAAAGATGGGCAGGTAGCCCAGGAGGAAA
  CAGGTCCTGAAAAACCTTCTGTGACCAGAGAGAAGAATGTGGAACCTGAGCAGCCAAGCACATCGAAGAA
  TGTCACCGAGGAAGAGCTGGAGGACTGGCTGGACAGCATGATTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217465 representing NM_028844
  Red=Cloning site Green=Tags(s)
  
  MQAERGARGGRGRRGGRERPGGDREPVGAATALARGGCGDGGGRRGRGRGFRRGRGGGGLRGGRWEPGGR
  GGGASTRVEEDSDSETYGEENDEQGNFSRRKIVSNWDRYQDTEKEVNGESGESQRGTDFSVLLSSAGDSF
  SQFRFAEEKEWDGETSCPKQNSALYVDSESLVRALEQLPLAVRLNVASELIQTTIPLELPQVKPRRNDDG
  KELGMHLRGPISELRSAAGACPRSLGRGSLRQSPLEGLQKAPTPTQSVADHLEEELDMLLHLDAPVQEEG
  NISPDQTSRDQEPEKDGQVAQEETGPEKPSVTREKNVEPEQPSTSKNVTEEELEDWLDSMIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028844
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028844.3, NP_083120.2
• RefSeq Size: 1432 bp
• RefSeq ORF: 1029 bp
• Locus ID: 74268
• UniProt ID: Q9D9K3
• Cytogenetics: 2 E3
• MW: 37.6 kDa
• Gene Summary: Protects against apoptosis mediated by Apaf-1.[UniProtKB/Swiss-Prot Function]