Adam33 (NM_033615) Mouse Tagged ORF Clone

CAT#: MR217277

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Adam33 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 33 (Adam33), transcript variant 1



  "NM_033615" in other vectors (4)

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Size
    • 10 ug


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Specifications

Product Data
Product Name Adam33 (NM_033615) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Adam33
Synonyms Adaml
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR217277 representing NM_033615
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTCGAGGTGCGGGAGACCCGGGGGGTCTCCGGTGCTGCTATTGCTGCCGCTGTTGCTGCCCTCGT
GTCCGCTGCGGAGCGCTCGGATGTTTCCAGGAAATGCCCATGGAGAGCTAGTCACTCCCCACTGGATCCT
GGAGGGCAGACTCTGGCTCAAGGTCACCCTGGAGGAGCCGATCTTGAAGCCTGACTCGGTGCTGGTGGCT
TTAGAGGCTGAAGGCCAGGATCTCCTGCTTGAACTGGAGAAGAAGCACAAGCTTCTGGCCCCAGGATACA
CAGAAACCCACTACAGGCCAGATGGGCATCCGGTAGTGCTGTCCCCCAACCACACGGATCATTGCCAATA
TCACGGGCGTGTGAGGGGCTTCCGGGAATCCTGGGTGGTTCTCAGCACCTGCTCTGGGATGAGTGGCCTT
ATTGTGCTCAGCAGCAAAGTCAGCTATTATCTGCAACCTCGGACTCCTGGGGATACCAAAGACTTCCCAA
CCCACGAGATCTTCCGGATGGAGCAGTTGTTCACCTGGAGAGGGGTCCAGAGAGACAAGAACTCCCAATA
CAAAGCAGGAATGGCCAGTCTTCCTCATGTCCCCCAGAGCCGGGTGAGGCGAGAGGCGCGCAGGAGTCCC
AGGTACCTGGAACTGTACATAGTGGCTGACCACACCCTGTTCTTGCTTCAGCATCAGAACTTGAACCACA
CGAGACAGCGCCTCCTGGAGGTTGCCAATTGCGTGGACCAGATTCTCAGGACTCTGGATATACAGTTGGT
GTTGACCGGGCTGGAAGTGTGGACCGAGCAGGATCTCAGTCGCATCACTCAGGACGCAAACGAAACGCTC
TGGGCTTTCCTACAGTGGCGCCGCGGGGTGTGGGCCAGGAGACCACACGACTCCACACAACTGCTCACGG
GCCGCACCTTCCAGGGTACCACGGTGGGCCTGGCACCTGTGGAGGGCATATGCCGCGCGGAGAGCTCCGG
AGGTGTGAGCACAGACCACTCGGAACTCCCCATCGGCACAGCAGCCACCATGGCCCACGAGATAGGCCAC
AGCCTGGGCCTCCACCATGATCCCGAGGGCTGCTGCGTGCAGGCCGATGCAGAGCAAGGAGGCTGCGTCA
TGGAGGCAGCCACAGGGCACCCTTTCCCGCGCGTCTTCAGCGCCTGCAGCCGCCGCCAGCTGCGCACCTT
CTTCCGCAAAGGGGGCGGTCCTTGCCTCTCCAACACCTCGGCGCCGGGGCTCCTGGTGCTGCCCAGCCGC
TGCGGAAACGGCTTCTTGGAAGCAGGAGAAGAGTGCGACTGCGGTTCTGGCCAGAAGTGCCCAGACCCCT
GCTGCTTTGCCCACAATTGCTCCCTGCGTGCGGGGGCTCAATGTGCCCACGGTGATTGCTGTGCGAGGTG
CCTGTTAAAGTCCGCGGGCACGCCTTGTCGTCCTGCTGCGACTGACTGCGATCTCCCCGAGTTCTGCACC
GGCACCTCCCCGTATTGCCCCGCAGATGTTTACCTACTGGATGGCTCACCCTGCGCTGAGGGTCGCGGCT
ATTGCCTAGACGGCTGGTGTCCCACGCTGGAGCAGCAGTGCCAGCAGCTATGGGGGCCTGGGTCCAAGCC
GGCCCCAGAGCCATGTTTCCAGCAGATGAACTCCATGGGGAATTCGCAAGGGAACTGTGGCCAGGACCAC
AAGGGTAGCTTCCTGCCTTGTGCTCAGAGGGACGCTCTGTGTGGGAAACTGCTGTGCCAGGGAGGGGAGC
CGAACCCACTAGTGCCGCACATAGTGACTATGGACTCCACAATTCTCCTAGAGGGCCGCGAAGTGGTTTG
CCGAGGGGCCTTTGTGCTCCCAGATAGTCACCTGGACCAGCTTGACTTGGGTCTGGTAGAGCCAGGCACC
GGCTGTGGACCTAGAATGGTGTGCCAGGACAGGCACTGTCAGAATGCTACCTCCCAGGAGCTGGAACGTT
GCTTGACTGCCTGCCATAACGGTGGGGTTTGCAATAGCAATCGTAACTGTCACTGTGCTGCTGGCTGGGC
TCCACCCTTCTGTGACAAGCCTGGCTTGGGTGGTAGCGTGGATAGTGGCCCTGCACAGTCTGCAAACCGA
GATGCCTTCCCCTTGGCCATGCTCCTCAGCTTCCTGCTGCCTCTGCTCCCTGGGGCTGGCCTAGCCTGGT
GCTACTACCAGCTCCCAACATTCTGTCATCGAAGGGGACTGTGCTGCAGGAGGGACCCCCTATGGAATAG
AGACATACCCCTGGGCAGTGTGCATCCGGTGGAGTTTGGCTCCATCATCACTGGAGAGCCCTCGCCCCCT
CCCCCATGGACCTCTTGCCAACAGCGTTCGCACCCTCCATCTCTTGACTTGCTCTCAGACCCTGCGAACT
CTGAGCTTACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR217277 representing NM_033615
Red=Cloning site Green=Tags(s)

MGSRCGRPGGSPVLLLLPLLLPSCPLRSARMFPGNAHGELVTPHWILEGRLWLKVTLEEPILKPDSVLVA
LEAEGQDLLLELEKKHKLLAPGYTETHYRPDGHPVVLSPNHTDHCQYHGRVRGFRESWVVLSTCSGMSGL
IVLSSKVSYYLQPRTPGDTKDFPTHEIFRMEQLFTWRGVQRDKNSQYKAGMASLPHVPQSRVRREARRSP
RYLELYIVADHTLFLLQHQNLNHTRQRLLEVANCVDQILRTLDIQLVLTGLEVWTEQDLSRITQDANETL
WAFLQWRRGVWARRPHDSTQLLTGRTFQGTTVGLAPVEGICRAESSGGVSTDHSELPIGTAATMAHEIGH
SLGLHHDPEGCCVQADAEQGGCVMEAATGHPFPRVFSACSRRQLRTFFRKGGGPCLSNTSAPGLLVLPSR
CGNGFLEAGEECDCGSGQKCPDPCCFAHNCSLRAGAQCAHGDCCARCLLKSAGTPCRPAATDCDLPEFCT
GTSPYCPADVYLLDGSPCAEGRGYCLDGWCPTLEQQCQQLWGPGSKPAPEPCFQQMNSMGNSQGNCGQDH
KGSFLPCAQRDALCGKLLCQGGEPNPLVPHIVTMDSTILLEGREVVCRGAFVLPDSHLDQLDLGLVEPGT
GCGPRMVCQDRHCQNATSQELERCLTACHNGGVCNSNRNCHCAAGWAPPFCDKPGLGGSVDSGPAQSANR
DAFPLAMLLSFLLPLLPGAGLAWCYYQLPTFCHRRGLCCRRDPLWNRDIPLGSVHPVEFGSIITGEPSPP
PPWTSCQQRSHPPSLDLLSDPANSELT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_033615
ORF Size 2391 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033615.1, NM_033615.2, NM_033615.3, NP_291093.2
RefSeq Size 3165 bp
RefSeq ORF 2394 bp
Locus ID 110751
Cytogenetics 2 63.26 cM
MW 87.4 kDa
Gene Summary This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. This gene is widely expressed, most highly in the adult brain, heart, kidney, lung and testis. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional metalloprotease enzyme. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016]

Citations (2)

Other Versions

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