Hnrnpf (NM_001166432) Mouse Tagged ORF Clone

CAT#: MR217122

Hnrnpf (Myc-DDK-tagged) - Mouse heterogeneous nuclear ribonucleoprotein F (Hnrnpf), transcript variant 7

Product Images

Circular map for MR217122

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hnrnpf
• Synonyms: 4833420I20Rik; AA407306; Hnrpf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR217122 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCTGGGCCCTGAGGGAGGTGAAGGCTATGTGGTCAAACTCCGTGGCCTACCCTGGTCCTGCTCAA
  TTGAGGACGTACAAAACTTCCTCTCCGACTGCACAATTCATGATGGGGTCGCAGGTGTTCATTTCATTTA
  TACTAGAGAAGGCAGGCAGAGTGGTGAGGCTTTTGTTGAACTTGAGTCAGAAGATGATGTAAAATTGGCT
  CTGAAAAAAGACAGGGAAAGCATGGGACACCGGTATATTGAGGTGTTCAAGTCACACAGAACCGAGATGG
  ATTGGGTGTTGAAGCACAGTGGTCCAAACAGCGCCGACAGTGCCAATGATGGCTTTGTGAGGCTTCGGGG
  ACTCCCATTTGGATGCACAAAGGAAGAAATCGTTCAGTTCTTCTCAGGGTTGGAAATTGTGCCAAACGGG
  ATCACACTACCTGTGGACCCGGAAGGCAAGATTACAGGGGAGGCCTTCGTTCAGTTTGCCTCACAAGAGT
  TAGCTGAGAAAGCTTTAGGGAAGCACAAGGAGAGAATAGGGCACAGGTATATTGAAGTGTTTAAGAGCAG
  TCAGGAGGAAGTTAGATCATACTCAGATCCACCTCTGAAGTTTATGTCTGTGCAAAGGCCTGGGCCTTAT
  GACAGGCCTGGCACAGCCCGGAGGTACATTGGCATTGTGAAACAGGCAGGTCTGGATAGGATGAGGTCTG
  GTGCCTATAGTGCAGGCTATGGGGGCTATGAAGAATACAGTGGCCTCAGTGATGGCTATGGCTTCACCAC
  TGACCTGTTTGGGAGAGACCTCAGCTATTGTCTCTCAGGAATGTATGACCACAGATATGGAGACAGCGAG
  TTCACAGTGCAGAGCACCACCGGCCACTGCGTCCACATGAGAGGGCTGCCCTACAAAGCAACGGAGAACG
  ACATTTACAACTTCTTCTCTCCACTCAACCCTGTGAGAGTTCATATTGAGATTGGTCCTGATGGAAGAGT
  GACGGGAGAAGCTGATGTTGAGTTTGCTACTCATGAAGAAGCAGTGGCAGCTATGTCCAAGGACAGGGCC
  AACATGCAGCACAGATACATAGAACTCTTCCTGAATTCAACAACAGGGGCTAGCAATGGGGCTTATAGCA
  GCCAGGTGATGCAGGGCATGGGCGTGTCAGCTGCCCAGGCAACTTACAGTGGCCTGGAGAGCCAGTCAGT
  GAGTGGCTGTTACGGGGCCGGCTACAGCGGTCAGAACAGCATGGGCGGATATGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR217122 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMLGPEGGEGYVVKLRGLPWSCSIEDVQNFLSDCTIHDGVAGVHFIYTREGRQSGEAFVELESEDDVKLA
  LKKDRESMGHRYIEVFKSHRTEMDWVLKHSGPNSADSANDGFVRLRGLPFGCTKEEIVQFFSGLEIVPNG
  ITLPVDPEGKITGEAFVQFASQELAEKALGKHKERIGHRYIEVFKSSQEEVRSYSDPPLKFMSVQRPGPY
  DRPGTARRYIGIVKQAGLDRMRSGAYSAGYGGYEEYSGLSDGYGFTTDLFGRDLSYCLSGMYDHRYGDSE
  FTVQSTTGHCVHMRGLPYKATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEEAVAAMSKDRA
  NMQHRYIELFLNSTTGASNGAYSSQVMQGMGVSAAQATYSGLESQSVSGCYGAGYSGQNSMGGYD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166432
• ORF Size: 1248 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166432.1, NP_001159904.1
• RefSeq Size: 2288 bp
• RefSeq ORF: 1248 bp
• Locus ID: 98758
• UniProt ID: Q9Z2X1
• Cytogenetics: 6 F1
• MW: 45.7 kDa
• Gene Summary: Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state (By similarity).[UniProtKB/Swiss-Prot Function]