Arid5a (NM_001172206) Mouse Tagged ORF Clone

CAT#: MR216827

Arid5a (Myc-DDK-tagged) - Mouse AT rich interactive domain 5A (MRF1-like) (Arid5a), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arid5a
• Synonyms: D430024K22Rik; Mrf1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216827 representing NM_001172206
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCTCCGGCCAAAGGGAACACAGAGCAGTCAGAAGAAGGTGACCTCCCGCAGCTTCCTGTATCCC
  CCAAGCCAGATGATGAGCAGAGCAGGAGCCAGAGCCCCACCCAGCTCCAGGACTCCCCTGAGGCAGGTGG
  GGAGCAGGAGGAGGAACAGGCCTTCCTGGTCAGCCTCTACAAGTTCATGAAGGAGCGACACACGCCCATC
  GAGAGGGTGCCCCATCTTGGCTTCAAGCAGATTAACCTGTGGAAGATCTACAAGGCAGTGGAGAAGCTGG
  GGGCCTATGAGCTGGTGACAGGCCGCCGCCTCTGGAAGAACGTGTATGATGAACTTGGCGGTAGCCCAGG
  CAGCACCAGTGCGGCCACATGCACACGCCGCCACTATGAGAGGCTGGTCCTCCCATATGTGCGGCATCTG
  AAGGGGGAGGACGACAAGCCACTGCCTCCTACCAAGCCCAGGAAGCAATACAAGATGGCCAAGGAGCTGA
  GGGGAGACGATGGGACCACTGAGAAGCTGAAGAAGGCCAAGGACTCAGAGGAGAGGCGGGTGGAGCAGAC
  CACGCCAGGAAAGACCAAATCAGATGCCACTGGCCAGACACAGCTTCCCTGCCAGGGATCCTCGAGGGAC
  AGCACAGAACAGCTGGGCCCAGTATCTGGACCCTCTCCACCACTCACGGGTGCTAGTAGCTGCCCTGAGG
  CCTACAAGCGGCTCTTGTCAAGCTTTTACTGCAAAGGGGCGCATGGCATCATGTCACCACTGGCCAAAAA
  GAAACTCCTGGCCCAGGTCAGCAAGGCAGAGGCCTTGCAGTGCCAAGAAGAGGGCTGTCGCCATGGAGCA
  AGGAGCCCCAACAAGGACATTCAAGACAGTCCCCAGAACCTAAGAGGGCCGGCTGAGAACTCTGAACACC
  AGCTAACCCCCCGGGAAGGATTGCAGGCCCCTGGTGGGAGCACCAGGATGGAGGCCCAAGTGGGCCCCTG
  CCCTACAGCCCCCATGTTCTCAGGCTGTTTTCATGCGTACCCCACCGAGGTGCTGAAACCTGTCAGCCAG
  CACCCTAGGGACTTCTTCTCCGGCCTTAAAGACAGGGTGCTGTTGGGACCACCTGGTAAAGAAGAAGGTC
  CGACAACCAAAGAGTCCCATCTGGTGTGGGGTGGGGATGCCAACCACCCCTCTGCATTCCATAAAGGCAG
  CACAAGAAAAAGAAGTTTCTACCCCAAACCCAAAGCCTGCTGGGTGTCTCCCATGGCCAAGGTCCCTACT
  GAGAGGCCTGGAGCCCCATCCCCTCATCCCAGTAGCCCAGGTCTTGGCAGTAAGCGCGGCTTGGAAGAAG
  AGGGATTCGCTCATGGTGGCAAGAAACTGAGGGCAGTGTCTCCCTTTCTGAAGGAGGTGGATTCCAAGGA
  GACTGGGGGCAAGCCTGCAGCCCCTGGCTTGGCTGTATCCTGTCTACTGGGCCCAACCCCGGGGCCCACT
  CCTCCAGAGGCCTACAGGGGCACCATGCTGCGGTGTCCTCTAAACTTCACCGGTAGCGCAGACCCTCTGA
  AGGGCCAGGCCTCACTCCCCTTCAGCCCCCTGGTCATCCCTGCTTTCCCAGCCCACCTTCTGGCTACAAC
  AGGCTCCTCACCTATGGCTGCCAGCCTGATGCATTTCCCTCCCACGCCCTATGACGCTGTCCTACGCAAC
  AGACTGGGTCCAGCTTCGTCTGCCTGGCACATGCCACCCGTCACAACCTATGCGGCACCTCACTTCTTCC
  ACCTCAACACCAAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216827 representing NM_001172206
  Red=Cloning site Green=Tags(s)
  
  MAPPAKGNTEQSEEGDLPQLPVSPKPDDEQSRSQSPTQLQDSPEAGGEQEEEQAFLVSLYKFMKERHTPI
  ERVPHLGFKQINLWKIYKAVEKLGAYELVTGRRLWKNVYDELGGSPGSTSAATCTRRHYERLVLPYVRHL
  KGEDDKPLPPTKPRKQYKMAKELRGDDGTTEKLKKAKDSEERRVEQTTPGKTKSDATGQTQLPCQGSSRD
  STEQLGPVSGPSPPLTGASSCPEAYKRLLSSFYCKGAHGIMSPLAKKKLLAQVSKAEALQCQEEGCRHGA
  RSPNKDIQDSPQNLRGPAENSEHQLTPREGLQAPGGSTRMEAQVGPCPTAPMFSGCFHAYPTEVLKPVSQ
  HPRDFFSGLKDRVLLGPPGKEEGPTTKESHLVWGGDANHPSAFHKGSTRKRSFYPKPKACWVSPMAKVPT
  ERPGAPSPHPSSPGLGSKRGLEEEGFAHGGKKLRAVSPFLKEVDSKETGGKPAAPGLAVSCLLGPTPGPT
  PPEAYRGTMLRCPLNFTGSADPLKGQASLPFSPLVIPAFPAHLLATTGSSPMAASLMHFPPTPYDAVLRN
  RLGPASSAWHMPPVTTYAAPHFFHLNTKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172206
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172206.1, NP_001165677.1
• RefSeq Size: 5696 bp
• RefSeq ORF: 1770 bp
• Locus ID: 214855
• UniProt ID: Q3U108
• Cytogenetics: 1 B
• MW: 64.3 kDa
• Gene Summary: DNA-binding protein that may regulate transcription and act as a repressor by binding to AT-rich stretches in the promoter region of target genes (By similarity). May positively regulate chondrocyte-specific transcription such as of COL2A1 in collaboration with SOX9 and positively regulate histone H3 acetylation at chondrocyte-specific genes. May stimulate early-stage chondrocyte differentiation and inhibit later stage differention (PubMed:21346191). Can repress ESR1-mediated transcriptional activation; proposed to act as corepressor for selective nuclear hormone receptors (By similarity). As RNA-binding protein involved in the regulation of inflammatory response by stabilizing selective inflammation-related mRNAs, such as IL6, STAT3 and TBX21. Binds to stem loop structures located in the 3' UTRs of IL6, STAT3 and TBX21 mRNAs; at least for STAT3 prevents binding of ZC3H12A to the mRNA stem loop structure thus inhibiting its degradation activity. Contributes to elevated IL6 levels possibly implicated in autoimmunity processes. IL6-dependent stabilization of STAT3 mRNA may promote differentiation of naive CD4+ T-cells into T-helper Th17 cells (PubMed:23676272, PubMed:27022145). In CD4+ T-cells may also inhibit RORC-induced Th17 cell differentiation independently of IL6 signaling (PubMed:24782182). Stabilization of TBX21 mRNA contributes to elevated interferon-gamma secretion in Th1 cells possibly implicated in the establishment of septic shock (PubMed:27671645).[UniProtKB/Swiss-Prot Function]