Acot7 (NM_001146057) Mouse Tagged ORF Clone

CAT#: MR216800

Acot7 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 7 (Acot7), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acot7
• Synonyms: 2410041A17Rik; Ach1; Act; Bach; Cte-II; CTE-IIa; Lach1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216800 representing NM_001146057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCAATGAAGCTGCTGGTCGGGACTCTGCGCCTCTGGGAGGTGGGGAGGCAGGTAGCCTTTTCGA
  GCCTGACGCCTGGCCAGGAGTGTTCGGGGCTCAGGAAAACATTCTGGGCTGCCATGAGGGCTGTCAGAAC
  CAGAGCAGATCACCAAAAGCTTGGACATTGCGTCACCATGGGAAGGATCATGCGTCCAGATGATGCCAAT
  GTGGCTGGCAATGTTCATGGAGGGACCATTCTGAAGATGATTGAGGAGGCCGGGGCCATCATCAGCACGC
  GGCACTGTAACAGCCAGAATGGGGAGCGCTGTGTGGCTGCCCTGGCACGGGTGGAGCGCACTGACTTCCT
  GTCACCCATGTGCATCGGCGAGGTGGCTCATGTCAGTGCAGAGATCACCTACACTTCCAAGCACTCTGTG
  GAGGTCCAGGTCCACGTGATGTCGGAGAACATCCTCACAGGTACCAAAAAGCTGACCAATAAAGCCACCT
  TGTGGTATGTGCCCCTGTCATTGAAGAATGTGGACAAGGTCCTTGAGGTGCCTCCCATTGTGTATTTACG
  GCAGGAGCAGGAGGAGGAGGGTCGGAAACGCTATGAAGCCCAGAAGCTGGAACGCATGGAGACCAAGTGG
  AGGAACGGAGACATTGTCCAGCCTGTCCTGAACCCAGAGCCGAACACGGTGAGCTACAGCCAGTCCAGCC
  TGATCCACCTGGTGGGGCCCTCGGACTGCACCCTTCATGGCTTCGTGCACGGAGGTGTCACCATGAAGCT
  CATGGATGAGGTGGCTGGGATTGTGGCTGCACGCCACTGCAAGACCAACATAGTAACTGCCTCTGTGGAT
  GCCATCAATTTCCACGACAAGATCCGGAAAGGCTGTGTCATCACCATCTCCGGACGCATGACCTTCACAA
  GCAATAAGTCCATGGAGATTGAGGTCCTGGTGGACGCTGACCCTGTGGTGGACAACTCACAAAAGCGCTA
  CCGGGCCGCCAGTGCCTTCTTCACCTACGTGTCCCTGAACCAGGAGGGCAAGCCAATGCCTGTGCCTCAG
  CTTGTGCCAGAGACGGAGGATGAGAAGAAGCGCTTCGAAGAAGGCAAAGGCCGTTATCTGCAGATGAAGG
  CGAAGCGACAGGGCCACACAGAGCCTCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216800 representing NM_001146057
  Red=Cloning site Green=Tags(s)
  
  MSAMKLLVGTLRLWEVGRQVAFSSLTPGQECSGLRKTFWAAMRAVRTRADHQKLGHCVTMGRIMRPDDAN
  VAGNVHGGTILKMIEEAGAIISTRHCNSQNGERCVAALARVERTDFLSPMCIGEVAHVSAEITYTSKHSV
  EVQVHVMSENILTGTKKLTNKATLWYVPLSLKNVDKVLEVPPIVYLRQEQEEEGRKRYEAQKLERMETKW
  RNGDIVQPVLNPEPNTVSYSQSSLIHLVGPSDCTLHGFVHGGVTMKLMDEVAGIVAARHCKTNIVTASVD
  AINFHDKIRKGCVITISGRMTFTSNKSMEIEVLVDADPVVDNSQKRYRAASAFFTYVSLNQEGKPMPVPQ
  LVPETEDEKKRFEEGKGRYLQMKAKRQGHTEPQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146057
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146057.1, NP_001139529.1
• RefSeq Size: 1505 bp
• RefSeq ORF: 1155 bp
• Locus ID: 70025
• UniProt ID: Q91V12
• Cytogenetics: 4 E2
• MW: 43.3 kDa
• Gene Summary: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:15288813). Acyl-coenzyme A thioesterase 7/ACOT7 preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18 (Probable). May play an important physiological function in brain (PubMed:15288813).[UniProtKB/Swiss-Prot Function]