Puf60 (NM_001164600) Mouse Tagged ORF Clone

CAT#: MR216560

Puf60 (Myc-DDK-tagged) - Mouse poly-U binding splicing factor 60 (Puf60), transcript variant 3

Product Images

Circular map for MR216560

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Puf60
• Synonyms: 2410104I19Rik; 2810454F19Rik; SIAHBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216560 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAATGGACAAAGCACAGGCACCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAGGCCC
  TCCAGAAGGCCAAGAAATATGCAATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAACAGACCATCGCGCA
  CCAGCAGCAGCAGCTCACCAACCTGCAGATGGCAGCTCAACGGCAGCGGGCACTGGCTATCATGTGCCGG
  GTCTATGTGGGCTCCATCTACTATGAGCTGGGAGAAGACACTATTCGCCAGGCCTTTGCTCCCTTTGGCC
  CCATCAAGAGCATTGATATGTCCTGGGACTCCGTTACCATGAAGCATAAGGGCTTTGCCTTCGTGGAGTA
  CGAGGTCCCAGAAGCTGCGCAGCTCGCTTTGGAGCAGATGAACTCTGTGATGCTTGGGGGCAGGAACATC
  AAGGTGGGAAGACCCAGCAACATTGGACAAGCCCAACCCATCATAGATCAGCTGGCTGAGGAAGCTAGGG
  CTTTCAACCGAATATATGTGGCCTCTGTACATCAAGACCTGTCTGATGATGACATCAAGAGTGTATTTGA
  AGCCTTTGGCAAGATCAAGTCTTGTACGCTGGCCCGGGACCCCACAACTGGCAAGCACAAGGGCTATGGT
  TTTATCGAATATGAGAAGGCCCAGTCGTCCCAGGATGCTGTGTCCTCCATGAACCTCTTTGATCTGGGTG
  GCCAGTACTTGAGGGTGGGCAAGGCCGTCACACCCCCCATGCCCCTGCTAACACCTGCCACGCCTGGAGG
  TCTCCCGCCTGCTGCTGCTGTGGCCGCAGCTGCAGCCACGGCCAAGATTACAGCTCAGGAAGCAGTGGCT
  GGAGCAGCAGTGCTGGGTACTTTAGCCACACCAGGACTAGTGTCCCCAGCACTGACACTGGCCCAGCCCT
  TAGGGGCCCTACCCCAGGCTGTCATGGCTGCCCAGGCCCCTGGAGTCATCACAGGTGTGACACCAGCCCG
  CCCTCCTATTCCGGTCACCATCCCCTCTGTGGGAGTAGTGAACCCCATTCTCGCCAGCCCGCCAACGTTG
  GGTCTGTTGGAGCCCAAGAAGGAGAAGGAAGAGGAGGAGCTGTTTCCTGAGTCAGAACGGCCAGAGATGT
  TGAGTGAGCAGGAGCACATGAGCATCTCGGGCAGCAGTGCTCGCCACATGGTCATGCAAAAGCTACTCAG
  AAAACAGGAGTCTACAGTGATGGTTCTCCGAAACATGGTGGACCCCAAGGACATCGATGACGACCTGGAG
  GGAGAGGTGACAGAGGAGTGTGGCAAATTTGGTGCTGTGAACCGGGTCATTATCTATCAAGAAAAACAGG
  GCGAAGAGGAAGATGCAGAAATCATCGTCAAGATTTTTGTGGAATTCTCCATGGCCTCAGAGACTCACAA
  GGCCATCCAGGCCCTCAATGGGCGCTGGTTTGGTGGTCGCAAGGTGGTGGCTGAAGTGTATGACCAGGAG
  CGTTTTGATAACAGCGACCTCTCCGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216560 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MENGQSTGTKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAQRQRALAIMCR
  VYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNI
  KVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYG
  FIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVA
  GAAVLGTLATPGLVSPALTLAQPLGALPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTL
  GLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLE
  GEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSMASETHKAIQALNGRWFGGRKVVAEVYDQE
  RFDNSDLSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001164600
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001164600.1, NP_001158072.1
• RefSeq Size: 1848 bp
• RefSeq ORF: 1500 bp
• Locus ID: 67959
• UniProt ID: Q3UEB3
• Cytogenetics: 15 D3
• MW: 54 kDa
• Gene Summary: DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA (By similarity).[UniProtKB/Swiss-Prot Function]