Acot5 (NM_145444) Mouse Tagged ORF Clone

CAT#: MR216341

Acot5 (Myc-DDK-tagged) - Mouse acyl-CoA thioesterase 5 (Acot5)

Product Images

Circular map for MR216341

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acot5
• Synonyms: BC016076; PTE-Ic
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216341 representing NM_145444
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCCAACAGTAAGCCTGGAGCCCACGGGCCACAGCTGCTGGGACGAACCGCTGAGCATCGCTGTGC
  GCGGCTTGGCCCCGGAGCAGCCCGTCACACTGCGCACTGCCCTGCGCGACGAGAAGGGCGCGCTCTTCCG
  AGCCCACGCGCGCTACCGCGCGGACTCCCACGGCGAGCTGGACCTGGCGCGCACGCCCGCGCTGGGCGGC
  AGCTTCTCGGGGCTCGAGCCCATGGGGCTGCTCTGGGCCATGGAGCCCGATCGGCCTTTCTGGCGCCTGA
  TCAAGCGCGACGTGCAGACGCCCTTCGTGGTGGAGCTGGAGGTGCTGGACGGACACGAGCCCGACGGCGG
  GCGGCTGCTGGCGCGGGCGGTGCACGAGCGTCACTTCATGGCTCCCGGGGTGCGGCGCGTGCCCGTGCGC
  GAGGGCCGGGTGCGCGCCACGCTCTTTCTGCCCCCAGGAACTGGACCCTTTCCTGGGATCATAGACCTTT
  TTGGAGTTGGAGGTGGCCTTCTGGAGTATCGGGCGAGCCTGCTGGCTGGGAAGGGCTTTGCCGTCATGGC
  TCTGGCTTATTATAAATATGATGACCTCCCTAAGGTTATAGACATCCTTCATCTGGAGTACTTTGAGGAA
  GCTGTGACCTACCTGCTCAGTCACCCTCAGGTCAAGGGTCCAGGAGTTGGCCTGCTAGGGATTTCCAAAG
  GGGCTGAACTCTCCCTCTCCATGGCTTCTTTCCTGAAAGGCATCACGGCCGCTGTCGTAATCAATGGCGC
  CACGGTCAACGTTATTTCAACATTATACTACAAGGAAGAGAGTCTGCCTGGTTTGGGCATGCACCTAGAA
  AGGATCAAGGTGACCAAAGATGGGTTTAAAGATATTATAGATATCCTGAATGTCCCTCTAGAAGCTCCAG
  ACCAGAAGAGCCTCATCCCCTTGGAAAGGTCTGACACGGCCTTCCTGTTCCTCGTAGGTCAGGACGACCA
  CAACTGGAAGAGCGAGTTCTATGCCAGAGAGGCCTCCAAACGCCTGCAGGCGCACGGGAAGGAGAAGCCC
  CAGATTGTCTGCTACCCCAAAACAGGACACCACATTGAACCCCCTTACATCCCTTGGTCCATAGCTGCCC
  CCCACAGCTATTTTGATAAGCCTATCCTCCTTGGAGGGGAGCCTAGGGCTCATGCCATGGCCCAGGTGGA
  CGCCTGGCAGCGGCTCCAGACTTTTTTCCATAAACATTTGAGTGGGGACAAGAGGCCAAGCCCAGCAAAA
  CTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216341 representing NM_145444
  Red=Cloning site Green=Tags(s)
  
  MVPTVSLEPTGHSCWDEPLSIAVRGLAPEQPVTLRTALRDEKGALFRAHARYRADSHGELDLARTPALGG
  SFSGLEPMGLLWAMEPDRPFWRLIKRDVQTPFVVELEVLDGHEPDGGRLLARAVHERHFMAPGVRRVPVR
  EGRVRATLFLPPGTGPFPGIIDLFGVGGGLLEYRASLLAGKGFAVMALAYYKYDDLPKVIDILHLEYFEE
  AVTYLLSHPQVKGPGVGLLGISKGAELSLSMASFLKGITAAVVINGATVNVISTLYYKEESLPGLGMHLE
  RIKVTKDGFKDIIDILNVPLEAPDQKSLIPLERSDTAFLFLVGQDDHNWKSEFYAREASKRLQAHGKEKP
  QIVCYPKTGHHIEPPYIPWSIAAPHSYFDKPILLGGEPRAHAMAQVDAWQRLQTFFHKHLSGDKRPSPAK
  L
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145444
• ORF Size: 1263 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145444.3, NP_663419.3
• RefSeq Size: 1518 bp
• RefSeq ORF: 1266 bp
• Locus ID: 217698
• UniProt ID: Q6Q2Z6
• Cytogenetics: 12 D1
• MW: 47 kDa
• Gene Summary: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:16940157). Mainly active on medium-chain acyl-CoAs (PubMed:15007068). Seems to be involved in intraperoxisomal regulation of acyl-CoA levels, but not CoASH levels (PubMed:15007068). May have a function in termination of beta-oxidation of fatty acids (PubMed:15007068).[UniProtKB/Swiss-Prot Function]