Akap12 (NM_031185) Mouse Tagged ORF Clone

CAT#: MR216222

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Akap12 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein (gravin) 12 (Akap12)



  "NM_031185" in other vectors (2)

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USD 1,490.00


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    • 10 ug


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Specifications

Product Data
Product Name Akap12 (NM_031185) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Akap12
Synonyms AI317366; Srcs5; SSeCKS; Tsga12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR216222 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGCAGGCAGTTCCACCGAGCAGCGGAGCCCCGAGCAGCCGGCGGAGAGCGACACGCCGAGCGAGC
TGGAGCTCAGTGGCCATGGGCCCGCAGCGGAAGCGTCGGGAGCAGCTGGAGATCCCGCTGACGCGGACCC
CGCCACCAAGCTCCCACAGAAGAATGGTCAGCTGTCTGCCGTCAATGGTGTAGCTGAACAAGAAGATGTC
CACGTCCAAGAGGAAAGCCAGGATGGGCAAGAGGAAGAAGTCACTGTTGAAGATGTTGGACAGAGAGAGT
CAGAAGATGTGAAAGAAAAAGACCGAGCTAAAGAAATGGCAGCCAGTTCCACAGTTGTTGAAGATATCAC
AAAGGACGAGCAGGAGGAAACACCGGAAATAATCGAACAGATCCCTGCTTCAGAGAGCAATGTGGAAGAA
ATGGCGCAGGCTGCTGAGTCCCAAGCTAATGACGTCGGCTTCAAGAAGGTATTTAAATTTGTTGGTTTTA
AATTCACGGTGAAGAAGGATAAAAACGAAAAGTCAGATACCGTCCAGCTACTCACTGTCAAGAAGGATGA
AGGCGAAGGGGCAGAAGCCTCCGTCGGAGCAGGAGACCACCAAGAGCCCGGAGTGGAGACCGTCGGCGAA
TCAGCATCCAAAGAAAGTGAGCTGAAGCAATCCACAGAGAAGCAAGAAGGCACCCTGAAGCAAGCACAGA
GCAGCACAGAAATTCCCCTTCAAGCCGAATCTGGTCAAGGGACCGAGGAAGAAGCAGCCAAAGATGGAGA
AGAAAACCGAGAGAAAGAACCTACCAAGCCCCTAGAATCTCCGACCAGCCCTGTCAGCAATGAGACAACA
TCTTCCTTCAAGAAATTCTTCACTCACGGCTGGGCCGGCTGGCGCAAGAAGACCAGCTTCAAGAAACCAA
AGGAAGATGATCTGGAAACTTCCGAGAAGAGAAAGGAGCAAGAGGCTGAAAAAGTAGACGAGGAAGAAGG
GGAAAAGACAGAGCCAGCCCCAGCCGAGGAGCAGGAGCCTGCAGAAGGCACAGACCAGGCCAGGTTGTCA
GCCGACTATGAGAAGGTGGAGTTGCCTTTGGAAGACCAGGTCGGTGACCTGGAGGCATTGTCGGAGAAGT
GTGCTCCTTTGGCAACGGAAGTGTTTGATGAGAAGACGGAAGCCCACCAAGAAGTTGTTGCAGAGGTCCA
CGTGAGCACCGTGGAGAAGATGACGAAAGGGCAAGGAGGAGCAGAGGTGGAAGGGGATGTGGTGGTGGAA
GGATCGGGAGAATCCTTGCCCCCTGAGAAACTGGCTGAGACCCAGGAGGTCCCCCAGGAAGCTGAGCCTG
TGGAGGAGCTGATGAAGACCAAAGAAGTATGCGTCTCTGGGGGTGACCATACTCAGCTGACAGATCTAAG
TCCTGAAGAGAAGATGCTACCCAAACACCCCGAAGGCATTGTCAGTGAGGTGGAGATGCTGTCCTCTCAG
GAGAGAATCAAGGTACAGGGAAGTCCCCTGAAGAAGCTCTTCAGCAGTTCGGGCTTAAAGAAGCTCTCCG
GGAAGAAGCAGAAGGGGAAGAGAGGAGGAGGCGGGGGAGATGAAGAGCCAGGAGAATACCAACACATTCA
AACCGAGTCCCCAGAGAGTGCTGACGAGCAGAAGGGAGAGAGCTCTGCCTCTTCCCCTGAAGAGCCCGAG
GAGATCGCGTGTCTGGAGAAGGGGCCATCGGAAGCACCCCAGGAAGCGGAAGCTGAGGAAGGAGCGACTT
CCGACGGAGAGAAGAAAAGGGAAGGGATCACCCCCTGGGCATCCTTCAAAAAGATGGTGACACCCAAGAA
ACGGGTCCGAAGACCTTCTGAGAGCGACAAGGAAGAAGAGCTGGATAAGGTCAAGAGTGCCACCTTGTCC
TCCACGGAGAGCACGGCGTCTGGAATGCAGGATGAGGTCAGAGCGGTTGGCGAGGAGCAAAGGTCAGAGG
AGCCAAAGCGCAGGGTGGATACTTCAGTGTCTTGGGAGGCGTTGATTTGTGTCGGATCGTCCAAGAAGAG
AGCGAGGAAGGCATCCTCTTCAGATGATGAAGGAGGGCCAAGAACACTGGGAGGGGATGGCCACAGAGCG
GAGGAGGCTAGCAAAGACAAAGAAGCAGATGCTCTTCCTGCCAGCACCCAGGAACAAGACCAAGCGCACG
GAAGTTCCTCACCCGAGCCAGCTGGAAGCCCTTCTGAAGGGGAGGGCGTCTCCACCTGGGAGTCATTTAA
GAGATTAGTCACTCCACGAAAAAAATCCAAGTCAAAACTGGAAGAGAGAGCCGAAGACTCCGGTGCAGAG
CAGTTGGCCTCCGAGATCGAACCAAGTAGAGAGGAATCTTGGGTTTCCATTAAGAAATTTATTCCTGGAC
GGCGGAAGAAAAGGGCAGATGGGAAGCAAGAACAGGCCGCCGTTGAAGACTCGGGGCCAGGAGAGATCAA
TGAGGACGACCCCGACGTCCCAGCTGTTGTGCCTCTGTCTGAGTACGATGCGGTAGAGAGAGAGAAGCTG
GAAGCGCAGCGAGCTCAGGAGAACGTGGAGCTGCCCCAGCTGAAGGGGGCTGTGTATGTGTCTGAGGAGC
TTAGTAAGACTCTGGTTCACACCGTGAGTGTCGCGGTCATTGATGGGACCAGGGCAGTCACCAGTGCCGA
AGAGCGGTCCCCTTCGTGGATATCTGCTTCCATGACAGAACCTCTTGAGCACGCAGAGGGAGTGGCCACA
CCGCCTGTTGGAGAGGTCACTGAAAAAGACATCACTGCAGAAGCAACTCCTGCACTCGCCCAGACTTTAC
CAGGGGGCAAAGATGCCCATGACGACATAGTCACCAGTGAAGTGGATTTTACCTCAGAAGCAGTGACAGC
CGCAGAAACCACAGAGGCGCTCCGCGCTGAAGAACTTACCGAAGCATCAGGGGCAGAAGAGACCACAGAC
ATGGTGTCTGCAGTTTCCCAGCTGTCCGACTCCCCGGACACCACAGAGGAAGCCACCCCAGTTCAGGAGG
TAGAGGGTGGCATGCTAGATACGGAAGAACAGGAGCGCCAGACGCAGGCCGTCCTCCAAGCCGTTGCAGA
CAAAGTGAAAGAGGACTCCCAGGTGCCTGCAACCCAGACTCTGCAGAGAGCAGGGCCGAAAGCACTGGAG
AAGGTGGAGGAGGTAGAGGAGGACTCCGAGGTGCTGGCTACCGAGAAAGAGAAGGATGTTGTGCCGGAAG
GACCCGTGCAGGAAGCTGAAACTGAGCATCTTGCACAGGGCTCCGAGACTGTACAGGCTACCCCAGAGAG
CCTTGAAGTTCCTGAAGTCACAGAGGATGTAGACCGTGCCACCACATGCCAGGTTATCAAGCACCAGCAG
CTGATGGAACAGGCTGTGGCCCCTGAGTCATCTGAAACCTTGACAGACAGTGAGACAAATGGAAGTACTC
CCCTCGCAGATTCAGACACTCCAAACGGGACACAGCAAGACGAGACCGTTGACAGCCAGGACAGTAATGC
CATTGCCGCCGTCAAGCAGTCACAGGTCACTGAAGAGGAGGCAGCTGCTGCTCAGACGGAGGGGCCTTCA
ACACCATCTAGTTTTCCAGCCCAGGAAGAACACAGGGAAAAACCAGGAAGGGATGTTCTAGAACCCACAC
AAGCGCTGGCTGCCGGGGCAGTGCCTATTCTGGCAAAGGCTGAGGTGGGTCAAGAGGGTGAGGCTGGCCA
GTTTGATGGAGAAAAAGTCAAAGACGGACAGTGTGTTAAAGAACTGGAGGTGCCTGTGCACACTGGACCC
AACAGTCAAAAGACTGCTGACTTGACACGTGACAGTGAAGTAATGGAAGTGGCCAGATGTCAGGAAACTG
AGAGTAATGAAGAACAGAGTATTAGCCCGGAGAAAAGAGAGATGGGAACCGACGTTGAAAAGGAGGAAAC
AGAGACCAAGACAGAGCAAGCCAGTGAAGAACATGAGCAGGAAACAGCTGCTCCTGAGCATGAAGGAACC
CACCCTAAGCCAGTCCTGACAGCTGACATGCCTCACTCAGAGAGGGGAAAGGCACTGGGCAGCCTTGAAG
GAAGCCCTTCTCTCCCAGACCAAGACAAAGCAGATTGCATAGAGGTTCAAGTTCAAAGCTCAGACACACC
AGTCACTCAAACAACCGAAGCTGTGAAAAAGGTCGAAGAAACTGTGGCAACTTCAGAGATGGATGAAAGT
TTGGAGTGTGCAGGTGCGCAATCATTACCAGCTGAGAAGCTCTCCGAAACCGGTGGCTACGGGACTCTTC
AGCATGGAGAGGACACCGTGCCCCAGGGGCCTGAGTCTCAGGCAGAGTCCATCCCAATAATAGTAACTCC
TGCTCCTGAAAGCATCCTACATTCTGACCTTCAAAGAGAAGTGAGCGCATCCCAGAAACAGAGATCAGAT
GAAGATAACAAGCCAGATGCTGGTCCTGATGCTGCCGGCAAGGAGAGTGCAGCAAGAGAGAAAATCCTCA
GGGCTGAACCTGAGATCTTGGAACTTGAGAGTAAGAGCAATAAGATTGTCCAGAGTGTCATCCAGACAGC
CGTCGACCAGTTTGCACGTACAGAAACAGCCCCCGAAACCCACGCTTCTGATTTACAGAATCAGGTTCCT
GTGATGCAGGCTGACAGCCAGGGAGCACAACAGATGCTGGACAAAGATGAAAGCGACCTTCAAGTCTCCC
CCCAAGATGGAACACTCAGTGCCGTAGCCCAGGAAGGACTTGCGGTTTCTGATAGTTCTGAAGGCATGAG
CAAGGCTTCAGAAATGATCACCACGCTTGCAGTTGAAAGTGCCAGTGTCAAAGAAAGTGTTGAGAAGCTG
CCTCTTCAGTGCAAAGATGAAAAGGAACATGCTGCTGACGGCCCCCAGCACCAAAGCTTAGCCAAGGCAG
AGGCGGATGCCTCTGGAAATCTAACCAAAGAGTCCCCAGACACCAACGGACCAAAGCTAACCGAGGAAGG
AGATGCCCTGAAAGAAGAAATGAACAAGGCCCAGACAGAAGAGGACGACCTACAGGAGCCAAAGGGAGAC
CTGACAGAATCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR216222 protein sequence
Red=Cloning site Green=Tags(s)

MGAGSSTEQRSPEQPAESDTPSELELSGHGPAAEASGAAGDPADADPATKLPQKNGQLSAVNGVAEQEDV
HVQEESQDGQEEEVTVEDVGQRESEDVKEKDRAKEMAASSTVVEDITKDEQEETPEIIEQIPASESNVEE
MAQAAESQANDVGFKKVFKFVGFKFTVKKDKNEKSDTVQLLTVKKDEGEGAEASVGAGDHQEPGVETVGE
SASKESELKQSTEKQEGTLKQAQSSTEIPLQAESGQGTEEEAAKDGEENREKEPTKPLESPTSPVSNETT
SSFKKFFTHGWAGWRKKTSFKKPKEDDLETSEKRKEQEAEKVDEEEGEKTEPAPAEEQEPAEGTDQARLS
ADYEKVELPLEDQVGDLEALSEKCAPLATEVFDEKTEAHQEVVAEVHVSTVEKMTKGQGGAEVEGDVVVE
GSGESLPPEKLAETQEVPQEAEPVEELMKTKEVCVSGGDHTQLTDLSPEEKMLPKHPEGIVSEVEMLSSQ
ERIKVQGSPLKKLFSSSGLKKLSGKKQKGKRGGGGGDEEPGEYQHIQTESPESADEQKGESSASSPEEPE
EIACLEKGPSEAPQEAEAEEGATSDGEKKREGITPWASFKKMVTPKKRVRRPSESDKEEELDKVKSATLS
STESTASGMQDEVRAVGEEQRSEEPKRRVDTSVSWEALICVGSSKKRARKASSSDDEGGPRTLGGDGHRA
EEASKDKEADALPASTQEQDQAHGSSSPEPAGSPSEGEGVSTWESFKRLVTPRKKSKSKLEERAEDSGAE
QLASEIEPSREESWVSIKKFIPGRRKKRADGKQEQAAVEDSGPGEINEDDPDVPAVVPLSEYDAVEREKL
EAQRAQENVELPQLKGAVYVSEELSKTLVHTVSVAVIDGTRAVTSAEERSPSWISASMTEPLEHAEGVAT
PPVGEVTEKDITAEATPALAQTLPGGKDAHDDIVTSEVDFTSEAVTAAETTEALRAEELTEASGAEETTD
MVSAVSQLSDSPDTTEEATPVQEVEGGMLDTEEQERQTQAVLQAVADKVKEDSQVPATQTLQRAGPKALE
KVEEVEEDSEVLATEKEKDVVPEGPVQEAETEHLAQGSETVQATPESLEVPEVTEDVDRATTCQVIKHQQ
LMEQAVAPESSETLTDSETNGSTPLADSDTPNGTQQDETVDSQDSNAIAAVKQSQVTEEEAAAAQTEGPS
TPSSFPAQEEHREKPGRDVLEPTQALAAGAVPILAKAEVGQEGEAGQFDGEKVKDGQCVKELEVPVHTGP
NSQKTADLTRDSEVMEVARCQETESNEEQSISPEKREMGTDVEKEETETKTEQASEEHEQETAAPEHEGT
HPKPVLTADMPHSERGKALGSLEGSPSLPDQDKADCIEVQVQSSDTPVTQTTEAVKKVEETVATSEMDES
LECAGAQSLPAEKLSETGGYGTLQHGEDTVPQGPESQAESIPIIVTPAPESILHSDLQREVSASQKQRSD
EDNKPDAGPDAAGKESAAREKILRAEPEILELESKSNKIVQSVIQTAVDQFARTETAPETHASDLQNQVP
VMQADSQGAQQMLDKDESDLQVSPQDGTLSAVAQEGLAVSDSSEGMSKASEMITTLAVESASVKESVEKL
PLQCKDEKEHAADGPQHQSLAKAEADASGNLTKESPDTNGPKLTEEGDALKEEMNKAQTEEDDLQEPKGD
LTES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_031185
ORF Size 5055 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031185.1, NM_031185.2, NM_031185.3, NP_112462.1
RefSeq Size 6262 bp
RefSeq ORF 5055 bp
Locus ID 83397
Cytogenetics 10 A1
MW 180.7 kDa

Citations (1)

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