Akap12 (NM_031185) Mouse Tagged ORF Clone

CAT#: MR216222

Akap12 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein (gravin) 12 (Akap12)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Akap12
• Synonyms: AI317366; Srcs5; SSeCKS; Tsga12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216222 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGCAGGCAGTTCCACCGAGCAGCGGAGCCCCGAGCAGCCGGCGGAGAGCGACACGCCGAGCGAGC
  TGGAGCTCAGTGGCCATGGGCCCGCAGCGGAAGCGTCGGGAGCAGCTGGAGATCCCGCTGACGCGGACCC
  CGCCACCAAGCTCCCACAGAAGAATGGTCAGCTGTCTGCCGTCAATGGTGTAGCTGAACAAGAAGATGTC
  CACGTCCAAGAGGAAAGCCAGGATGGGCAAGAGGAAGAAGTCACTGTTGAAGATGTTGGACAGAGAGAGT
  CAGAAGATGTGAAAGAAAAAGACCGAGCTAAAGAAATGGCAGCCAGTTCCACAGTTGTTGAAGATATCAC
  AAAGGACGAGCAGGAGGAAACACCGGAAATAATCGAACAGATCCCTGCTTCAGAGAGCAATGTGGAAGAA
  ATGGCGCAGGCTGCTGAGTCCCAAGCTAATGACGTCGGCTTCAAGAAGGTATTTAAATTTGTTGGTTTTA
  AATTCACGGTGAAGAAGGATAAAAACGAAAAGTCAGATACCGTCCAGCTACTCACTGTCAAGAAGGATGA
  AGGCGAAGGGGCAGAAGCCTCCGTCGGAGCAGGAGACCACCAAGAGCCCGGAGTGGAGACCGTCGGCGAA
  TCAGCATCCAAAGAAAGTGAGCTGAAGCAATCCACAGAGAAGCAAGAAGGCACCCTGAAGCAAGCACAGA
  GCAGCACAGAAATTCCCCTTCAAGCCGAATCTGGTCAAGGGACCGAGGAAGAAGCAGCCAAAGATGGAGA
  AGAAAACCGAGAGAAAGAACCTACCAAGCCCCTAGAATCTCCGACCAGCCCTGTCAGCAATGAGACAACA
  TCTTCCTTCAAGAAATTCTTCACTCACGGCTGGGCCGGCTGGCGCAAGAAGACCAGCTTCAAGAAACCAA
  AGGAAGATGATCTGGAAACTTCCGAGAAGAGAAAGGAGCAAGAGGCTGAAAAAGTAGACGAGGAAGAAGG
  GGAAAAGACAGAGCCAGCCCCAGCCGAGGAGCAGGAGCCTGCAGAAGGCACAGACCAGGCCAGGTTGTCA
  GCCGACTATGAGAAGGTGGAGTTGCCTTTGGAAGACCAGGTCGGTGACCTGGAGGCATTGTCGGAGAAGT
  GTGCTCCTTTGGCAACGGAAGTGTTTGATGAGAAGACGGAAGCCCACCAAGAAGTTGTTGCAGAGGTCCA
  CGTGAGCACCGTGGAGAAGATGACGAAAGGGCAAGGAGGAGCAGAGGTGGAAGGGGATGTGGTGGTGGAA
  GGATCGGGAGAATCCTTGCCCCCTGAGAAACTGGCTGAGACCCAGGAGGTCCCCCAGGAAGCTGAGCCTG
  TGGAGGAGCTGATGAAGACCAAAGAAGTATGCGTCTCTGGGGGTGACCATACTCAGCTGACAGATCTAAG
  TCCTGAAGAGAAGATGCTACCCAAACACCCCGAAGGCATTGTCAGTGAGGTGGAGATGCTGTCCTCTCAG
  GAGAGAATCAAGGTACAGGGAAGTCCCCTGAAGAAGCTCTTCAGCAGTTCGGGCTTAAAGAAGCTCTCCG
  GGAAGAAGCAGAAGGGGAAGAGAGGAGGAGGCGGGGGAGATGAAGAGCCAGGAGAATACCAACACATTCA
  AACCGAGTCCCCAGAGAGTGCTGACGAGCAGAAGGGAGAGAGCTCTGCCTCTTCCCCTGAAGAGCCCGAG
  GAGATCGCGTGTCTGGAGAAGGGGCCATCGGAAGCACCCCAGGAAGCGGAAGCTGAGGAAGGAGCGACTT
  CCGACGGAGAGAAGAAAAGGGAAGGGATCACCCCCTGGGCATCCTTCAAAAAGATGGTGACACCCAAGAA
  ACGGGTCCGAAGACCTTCTGAGAGCGACAAGGAAGAAGAGCTGGATAAGGTCAAGAGTGCCACCTTGTCC
  TCCACGGAGAGCACGGCGTCTGGAATGCAGGATGAGGTCAGAGCGGTTGGCGAGGAGCAAAGGTCAGAGG
  AGCCAAAGCGCAGGGTGGATACTTCAGTGTCTTGGGAGGCGTTGATTTGTGTCGGATCGTCCAAGAAGAG
  AGCGAGGAAGGCATCCTCTTCAGATGATGAAGGAGGGCCAAGAACACTGGGAGGGGATGGCCACAGAGCG
  GAGGAGGCTAGCAAAGACAAAGAAGCAGATGCTCTTCCTGCCAGCACCCAGGAACAAGACCAAGCGCACG
  GAAGTTCCTCACCCGAGCCAGCTGGAAGCCCTTCTGAAGGGGAGGGCGTCTCCACCTGGGAGTCATTTAA
  GAGATTAGTCACTCCACGAAAAAAATCCAAGTCAAAACTGGAAGAGAGAGCCGAAGACTCCGGTGCAGAG
  CAGTTGGCCTCCGAGATCGAACCAAGTAGAGAGGAATCTTGGGTTTCCATTAAGAAATTTATTCCTGGAC
  GGCGGAAGAAAAGGGCAGATGGGAAGCAAGAACAGGCCGCCGTTGAAGACTCGGGGCCAGGAGAGATCAA
  TGAGGACGACCCCGACGTCCCAGCTGTTGTGCCTCTGTCTGAGTACGATGCGGTAGAGAGAGAGAAGCTG
  GAAGCGCAGCGAGCTCAGGAGAACGTGGAGCTGCCCCAGCTGAAGGGGGCTGTGTATGTGTCTGAGGAGC
  TTAGTAAGACTCTGGTTCACACCGTGAGTGTCGCGGTCATTGATGGGACCAGGGCAGTCACCAGTGCCGA
  AGAGCGGTCCCCTTCGTGGATATCTGCTTCCATGACAGAACCTCTTGAGCACGCAGAGGGAGTGGCCACA
  CCGCCTGTTGGAGAGGTCACTGAAAAAGACATCACTGCAGAAGCAACTCCTGCACTCGCCCAGACTTTAC
  CAGGGGGCAAAGATGCCCATGACGACATAGTCACCAGTGAAGTGGATTTTACCTCAGAAGCAGTGACAGC
  CGCAGAAACCACAGAGGCGCTCCGCGCTGAAGAACTTACCGAAGCATCAGGGGCAGAAGAGACCACAGAC
  ATGGTGTCTGCAGTTTCCCAGCTGTCCGACTCCCCGGACACCACAGAGGAAGCCACCCCAGTTCAGGAGG
  TAGAGGGTGGCATGCTAGATACGGAAGAACAGGAGCGCCAGACGCAGGCCGTCCTCCAAGCCGTTGCAGA
  CAAAGTGAAAGAGGACTCCCAGGTGCCTGCAACCCAGACTCTGCAGAGAGCAGGGCCGAAAGCACTGGAG
  AAGGTGGAGGAGGTAGAGGAGGACTCCGAGGTGCTGGCTACCGAGAAAGAGAAGGATGTTGTGCCGGAAG
  GACCCGTGCAGGAAGCTGAAACTGAGCATCTTGCACAGGGCTCCGAGACTGTACAGGCTACCCCAGAGAG
  CCTTGAAGTTCCTGAAGTCACAGAGGATGTAGACCGTGCCACCACATGCCAGGTTATCAAGCACCAGCAG
  CTGATGGAACAGGCTGTGGCCCCTGAGTCATCTGAAACCTTGACAGACAGTGAGACAAATGGAAGTACTC
  CCCTCGCAGATTCAGACACTCCAAACGGGACACAGCAAGACGAGACCGTTGACAGCCAGGACAGTAATGC
  CATTGCCGCCGTCAAGCAGTCACAGGTCACTGAAGAGGAGGCAGCTGCTGCTCAGACGGAGGGGCCTTCA
  ACACCATCTAGTTTTCCAGCCCAGGAAGAACACAGGGAAAAACCAGGAAGGGATGTTCTAGAACCCACAC
  AAGCGCTGGCTGCCGGGGCAGTGCCTATTCTGGCAAAGGCTGAGGTGGGTCAAGAGGGTGAGGCTGGCCA
  GTTTGATGGAGAAAAAGTCAAAGACGGACAGTGTGTTAAAGAACTGGAGGTGCCTGTGCACACTGGACCC
  AACAGTCAAAAGACTGCTGACTTGACACGTGACAGTGAAGTAATGGAAGTGGCCAGATGTCAGGAAACTG
  AGAGTAATGAAGAACAGAGTATTAGCCCGGAGAAAAGAGAGATGGGAACCGACGTTGAAAAGGAGGAAAC
  AGAGACCAAGACAGAGCAAGCCAGTGAAGAACATGAGCAGGAAACAGCTGCTCCTGAGCATGAAGGAACC
  CACCCTAAGCCAGTCCTGACAGCTGACATGCCTCACTCAGAGAGGGGAAAGGCACTGGGCAGCCTTGAAG
  GAAGCCCTTCTCTCCCAGACCAAGACAAAGCAGATTGCATAGAGGTTCAAGTTCAAAGCTCAGACACACC
  AGTCACTCAAACAACCGAAGCTGTGAAAAAGGTCGAAGAAACTGTGGCAACTTCAGAGATGGATGAAAGT
  TTGGAGTGTGCAGGTGCGCAATCATTACCAGCTGAGAAGCTCTCCGAAACCGGTGGCTACGGGACTCTTC
  AGCATGGAGAGGACACCGTGCCCCAGGGGCCTGAGTCTCAGGCAGAGTCCATCCCAATAATAGTAACTCC
  TGCTCCTGAAAGCATCCTACATTCTGACCTTCAAAGAGAAGTGAGCGCATCCCAGAAACAGAGATCAGAT
  GAAGATAACAAGCCAGATGCTGGTCCTGATGCTGCCGGCAAGGAGAGTGCAGCAAGAGAGAAAATCCTCA
  GGGCTGAACCTGAGATCTTGGAACTTGAGAGTAAGAGCAATAAGATTGTCCAGAGTGTCATCCAGACAGC
  CGTCGACCAGTTTGCACGTACAGAAACAGCCCCCGAAACCCACGCTTCTGATTTACAGAATCAGGTTCCT
  GTGATGCAGGCTGACAGCCAGGGAGCACAACAGATGCTGGACAAAGATGAAAGCGACCTTCAAGTCTCCC
  CCCAAGATGGAACACTCAGTGCCGTAGCCCAGGAAGGACTTGCGGTTTCTGATAGTTCTGAAGGCATGAG
  CAAGGCTTCAGAAATGATCACCACGCTTGCAGTTGAAAGTGCCAGTGTCAAAGAAAGTGTTGAGAAGCTG
  CCTCTTCAGTGCAAAGATGAAAAGGAACATGCTGCTGACGGCCCCCAGCACCAAAGCTTAGCCAAGGCAG
  AGGCGGATGCCTCTGGAAATCTAACCAAAGAGTCCCCAGACACCAACGGACCAAAGCTAACCGAGGAAGG
  AGATGCCCTGAAAGAAGAAATGAACAAGGCCCAGACAGAAGAGGACGACCTACAGGAGCCAAAGGGAGAC
  CTGACAGAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216222 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGAGSSTEQRSPEQPAESDTPSELELSGHGPAAEASGAAGDPADADPATKLPQKNGQLSAVNGVAEQEDV
  HVQEESQDGQEEEVTVEDVGQRESEDVKEKDRAKEMAASSTVVEDITKDEQEETPEIIEQIPASESNVEE
  MAQAAESQANDVGFKKVFKFVGFKFTVKKDKNEKSDTVQLLTVKKDEGEGAEASVGAGDHQEPGVETVGE
  SASKESELKQSTEKQEGTLKQAQSSTEIPLQAESGQGTEEEAAKDGEENREKEPTKPLESPTSPVSNETT
  SSFKKFFTHGWAGWRKKTSFKKPKEDDLETSEKRKEQEAEKVDEEEGEKTEPAPAEEQEPAEGTDQARLS
  ADYEKVELPLEDQVGDLEALSEKCAPLATEVFDEKTEAHQEVVAEVHVSTVEKMTKGQGGAEVEGDVVVE
  GSGESLPPEKLAETQEVPQEAEPVEELMKTKEVCVSGGDHTQLTDLSPEEKMLPKHPEGIVSEVEMLSSQ
  ERIKVQGSPLKKLFSSSGLKKLSGKKQKGKRGGGGGDEEPGEYQHIQTESPESADEQKGESSASSPEEPE
  EIACLEKGPSEAPQEAEAEEGATSDGEKKREGITPWASFKKMVTPKKRVRRPSESDKEEELDKVKSATLS
  STESTASGMQDEVRAVGEEQRSEEPKRRVDTSVSWEALICVGSSKKRARKASSSDDEGGPRTLGGDGHRA
  EEASKDKEADALPASTQEQDQAHGSSSPEPAGSPSEGEGVSTWESFKRLVTPRKKSKSKLEERAEDSGAE
  QLASEIEPSREESWVSIKKFIPGRRKKRADGKQEQAAVEDSGPGEINEDDPDVPAVVPLSEYDAVEREKL
  EAQRAQENVELPQLKGAVYVSEELSKTLVHTVSVAVIDGTRAVTSAEERSPSWISASMTEPLEHAEGVAT
  PPVGEVTEKDITAEATPALAQTLPGGKDAHDDIVTSEVDFTSEAVTAAETTEALRAEELTEASGAEETTD
  MVSAVSQLSDSPDTTEEATPVQEVEGGMLDTEEQERQTQAVLQAVADKVKEDSQVPATQTLQRAGPKALE
  KVEEVEEDSEVLATEKEKDVVPEGPVQEAETEHLAQGSETVQATPESLEVPEVTEDVDRATTCQVIKHQQ
  LMEQAVAPESSETLTDSETNGSTPLADSDTPNGTQQDETVDSQDSNAIAAVKQSQVTEEEAAAAQTEGPS
  TPSSFPAQEEHREKPGRDVLEPTQALAAGAVPILAKAEVGQEGEAGQFDGEKVKDGQCVKELEVPVHTGP
  NSQKTADLTRDSEVMEVARCQETESNEEQSISPEKREMGTDVEKEETETKTEQASEEHEQETAAPEHEGT
  HPKPVLTADMPHSERGKALGSLEGSPSLPDQDKADCIEVQVQSSDTPVTQTTEAVKKVEETVATSEMDES
  LECAGAQSLPAEKLSETGGYGTLQHGEDTVPQGPESQAESIPIIVTPAPESILHSDLQREVSASQKQRSD
  EDNKPDAGPDAAGKESAAREKILRAEPEILELESKSNKIVQSVIQTAVDQFARTETAPETHASDLQNQVP
  VMQADSQGAQQMLDKDESDLQVSPQDGTLSAVAQEGLAVSDSSEGMSKASEMITTLAVESASVKESVEKL
  PLQCKDEKEHAADGPQHQSLAKAEADASGNLTKESPDTNGPKLTEEGDALKEEMNKAQTEEDDLQEPKGD
  LTES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031185
• ORF Size: 5055 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031185.3, NP_112462.1
• RefSeq Size: 6262 bp
• RefSeq ORF: 5055 bp
• Locus ID: 83397
• UniProt ID: Q9WTQ5
• Cytogenetics: 10 A1
• MW: 180.7 kDa
• Gene Summary: Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC).[UniProtKB/Swiss-Prot Function]