Asap2 (NM_001004364) Mouse Tagged ORF Clone

CAT#: MR216157

Asap2 (Myc-DDK-tagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (Asap2), transcript variant 2

Product Images

Circular map for MR216157

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asap2
• Synonyms: 6530401G17Rik; Ddef2; Gm592; Gm1523; PAG3; PAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216157 representing NM_001004364
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAGGACTACAAGGCGCCCACGGCCT
  CTAGCTTCACCACGCGCACGGCCCAGTGCCGGAACACCGTGGCGGCCATCGAGGAGGCTTTGGACGTGGA
  CCGGATGGTTCTCTACAAAATGAAGAAATCTGTGAAGGCAATCAATATCTCTGGGCTGGCACACGTGGAG
  AACGAGGAACAGTACACCCAGGCTCTGGAGAAGTTCGGTGGCAACTGTGTGTGCAGAGATGATCCAGACT
  TAGGAAGCGCGTTTCTGAAGTTCTCTGTGTTCACCAAGGAGTTGACGGCACTCTTCAAAAACCTGATTCA
  GAACATGAACAACATCATCTCGTTCCCTTTGGACAGTTTGCTGAAAGGAGATCTGAAAGGAGTAAAAGGG
  GATCTGAAAAAGCCCTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAAATAGAAAAGGAGA
  AAAAGGAGCATGCCAAGCTCCACGGGATGATTCGTACTGAAATAAGCGGGGCTGAGATTGCAGAGGAGAT
  GGAGAAGGAAAGACGGTTCTTCCAGCTGCAGATGTGTGAGTACCTGCTGAAGGTCAATGAAATCAAGGTG
  AAAAAGGGAGTGGATTTGCTTCAGAATCTGATCAAGTACTTTCACGCCCAGTGCAATTTTTTCCAGGATG
  GATTGAAAGCAGTAGAAAGCCTCAAGCCTTCCATCGAGACGCTCTCCACGGACCTCCACACCATCAAACA
  GGCCCAGGATGAGGAACGGCGACAGCTGATACAACTTCGAGATATTTTGAAGTCAGCATTGCAGGTGGAA
  CAGAAAGAGGACTCACAACTTCGCCAAAGTACAGCCTACAGCTTACATCAACCTCAGGGAAACAAGGAGC
  ATGGAACAGAGAGGAACGGGAACCTCTACAAGAAGAGCGATGGGATCCGGAAAGTGTGGCAGAAGAGGAA
  GTGTTCCGTTAAAAATGGCTTCCTCACCATCTCCCACGGCACTGCCAACCGGCCACCTGCCAAGCTCAAC
  CTGCTAACCTGCCAGGTGAAGACCAATCCTGAGGAGAAGAAGTGTTTCGACCTCATATCACATGACAGGA
  CGTACCACTTCCAAGCGGAAGACGAACAAGAATGTCAGATATGGATGTCTGTACTGCAGAACAGCAAGGA
  AGAAGCTCTGAACAACGCCTTTAAGGGTGATGACAACACTGGAGAAAATAACATCGTCCAAGAGCTGACC
  AAGGAGATCATCTCGGAGGTGCAGAGGATGACGGGCAATGACGTGTGCTGCGACTGTGGGGCACCAGATC
  CGACGTGGCTCTCTACCAACCTGGGCATCCTGACTTGCATCGAGTGCTCTGGGATCCACCGGGAGCTGGG
  GGTTCATTACTCCAGGATGCAGTCCCTGACATTAGATGTATTGGGAACGTCTGAGCTTCTGCTTGCCAAG
  AATATCGGGAATGCAGGCTTTAATGAAATTATGGAGTGTTGCCTACCGTCTGAGGACCCAGTCAAGCCCA
  ACCCAGGCAGTGACATGATTGCAAGGAAGGACTACATCACAGCCAAGTACATGGAGAGGAGATATGCACG
  GAAAAAGCATGCCGACACCGCGGCGAAGCTCCACAGCCTTTGCGAGGCCGTCAAGACCAGAGACATTTTT
  GGGTTACTCCAAGCTTATGCTGATGGTGTGGACCTGACAGAGAAAATCCCACTGGCCAATGGGCATGAGC
  CAGATGAGACAGCCCTCCATCTTGCAGTCAGATCTGTGGACCGGACTTCCCTTCACATTGTAGACTTCCT
  GGTCCAGAACAGTGGGAACCTGGATAAACAGACAGGCAAGGGCAGCACAGCCCTGCACTACTGCTGCCTG
  ACAGACAATGCTGAGTGCCTCAAGCTCCTCCTGCGGGGAAAAGCCTCCATTGAGATAGCCAATGAGTCAG
  GAGAGACCCCGTTGGACATTGCTAAGCGGCTCAAGCACGAACACTGTGAGGAACTGCTCACTCAGGCCTT
  GTCGGGGCGGTTTAACTCCCATGTTCACGTCGAGTATGAATGGCGGCTGCTGCACGAGGACCTGGATGAG
  AGCGACGACGATGTGGACGAGAAGCTACAGCCTAGTCCCAACCGACGGGAGGACCGGCCTGTTAGCTTCT
  ACCAGCTGGGGTCCAGCCAGTTTCAGTCCAATGCTGTGTCTTTGGCCAGAGATACTGCAAACCTCACCAA
  GGACAAGCAGAGGGGCTTTGGGCCCAGCATCTTGCAGAATGAGACCTATGGAGCCATCCTGAGTGGCAGC
  CCGCCCTCCTCCCAGTCAATACCCCCCAGCACCACCAGCGCACCCCCGCTCCCACCTCGGAATGTTGGCA
  AAGATCCCCTGACCACCACACCACCCCCTCCTGTGGCTAAGACATCAGGAACTCTAGAAGCCATGAACCA
  GCCGAGCAAGTCCTCCCAACCTGGGACCTCACAGAGCAAGCCCCCGCCCCTGCCACCTCAGCCACCCAGC
  CGCCTGCCTCAGAAGAAACCAGCTTCCGGGACTGACAAGCCAACCCCGCTGACCAACAAAGGACAGCCGA
  GAGGACCTGAAGCTTCGGGTCCTCTGTCCAACGCCATGGCCCTGCAGCCCCCAGCACCCATGCCTAGGAA
  GTCACAGGCGACCAAGTCAAAGCCCAAGCGAGTGAAAGCACTCTACAACTGCGTGGCTGACAACCCAGAC
  GAGCTGACTTTCTCTGAGGGCGACGTGATCATTGTGGATGGGGAAGAGGATCAGGAGTGGTGGATCGGCC
  ACATCGATGGAGAGCCCAGCCGCAAAGGAGCGTTTCCCGTATCGTTTGTGCACTTTATTGCTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216157 representing NM_001004364
  Red=Cloning site Green=Tags(s)
  
  MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEALDVDRMVLYKMKKSVKAINISGLAHVE
  NEEQYTQALEKFGGNCVCRDDPDLGSAFLKFSVFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKG
  DLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKV
  KKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSALQVE
  QKEDSQLRQSTAYSLHQPQGNKEHGTERNGNLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPAKLN
  LLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQELT
  KEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELLLAK
  NIGNAGFNEIMECCLPSEDPVKPNPGSDMIARKDYITAKYMERRYARKKHADTAAKLHSLCEAVKTRDIF
  GLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHYCCL
  TDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHEDLDE
  SDDDVDEKLQPSPNRREDRPVSFYQLGSSQFQSNAVSLARDTANLTKDKQRGFGPSILQNETYGAILSGS
  PPSSQSIPPSTTSAPPLPPRNVGKDPLTTTPPPPVAKTSGTLEAMNQPSKSSQPGTSQSKPPPLPPQPPS
  RLPQKKPASGTDKPTPLTNKGQPRGPEASGPLSNAMALQPPAPMPRKSQATKSKPKRVKALYNCVADNPD
  ELTFSEGDVIIVDGEEDQEWWIGHIDGEPSRKGAFPVSFVHFIAD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004364
• ORF Size: 2865 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004364.2, NP_001004364.2
• RefSeq Size: 5557 bp
• RefSeq ORF: 2868 bp
• Locus ID: 211914
• UniProt ID: Q7SIG6
• Cytogenetics: 12 A1.2-A1.3
• MW: 106.9 kDa
• Gene Summary: Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration (By similarity).[UniProtKB/Swiss-Prot Function]