Ptpn21 (NM_001146199) Mouse Tagged ORF Clone

CAT#: MR216090

Ptpn21 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, non-receptor type 21 (Ptpn21), transcript variant 2

Product Images

Circular map for MR216090

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ptpn21
• Synonyms: PTPD1; PTPRL10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216090 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACTGCCATTTGGATTGAAGTTGAAACGCACCCGACGCTACACTGTGTCCAGCAAGAGCTGCCTGG
  TTGCCCGGATCCAGCTGCTCAATAATGAGTTTGTGGAGTTCACTCTGTCTGTGGAGAGCACTGGCCAGGA
  GAGCCTAGAAGCTGTGGCCCAGCGCCTGGAGCTGAGAGAGGTCACTTACTTTAGCCTCTGGTACTACAAC
  AAGCAGAATCAGCGGCGCTGGGTGGATTTGGAGAAACCCCTGAAGAAGCAGCTGGACAAGCACGCACTGG
  AGCCTACCGTCTACTTCGGCGTGCTATTCTATGTGCCTTCTGTTTCCCAGCTGCAGCAGGAGATTACCAG
  GTATCAGTATTACCTGCAGCTGAAGAAAGATATTCTGGAAGGAAACCTTCCCTGTACATTAGAACACGCC
  ATTCAGCTGGCCGGCTTAGCTGTTCAAGCTGATTTTGGTGACTTTGATCAGTATGAATCCCAAGATTTTC
  TTCAGAAATTTGCCTTGCTCCCCGTGGCATGGCTACAGGATGAAAAAGTGCTGGAAGAAGCAGCCCAGAA
  AGTGGCCTTACTGCACCAGAAATACAGAGGGCTCACAGCTCCTGAGGCCGAGCTGCTGTACATGCAGGAG
  GTAGAGAGAATGGATGGCTACGGAGAGGAGAGCTACCCTGCCAAGGACAGCCAAGGAAGTGACATATCCA
  TTGGTGCCTGTCTCGATGGCATCTTTGTGAAACACAAGAACGGAAGGCCTCCTGTGGTGTTCAGGTGGCA
  CGACATTGCTAACATGTCGCATAACAAGTCCTTTTTTGCACTAGAGCTGGCAAATAAAGAGGAGACCATC
  CAGTTTCAAACTGAAGACATGGAGACAGCCAAGTACGTGTGGCGGCTGTGTGTGGCACGGCACAAATTCT
  ACAGACTGAACCAGTGTAGCCTGCAAACGCAGGCCGCCACGCTGAACTCAGTCAGGAGGGGCTCATCCTC
  CAGGATGTCTCTGCCTAAACCCCAGCCCTATGCGATGCCTCCCCCACCCCAGCTGCATTATAATGGACAT
  TATACAGAGCCATTTGCTTCTTCCCAAGACAACATCTTTGTGCCCAACAAGAATGGATTCTACTGTCACT
  CCCAGACAAGCCTGGATAGAACCCAGATTGACCTCAGTGGTCGCATCCGCAACGGCAGCGTCTACAGTGC
  ACACAGTACGAATTCCTTAAACACACTTCAGCCCTACCTGCAGCCCTCCCCCATGTCCTCCAACCCGAGT
  ATCACTGGCAGTGATGTCATGAGACCCGACTACATCCCGTCCCACCGGCACAGTGCGCTCATTCCCCCGT
  CTTACCGCCCGACCCCTGACTACGAGACCGTGATGAAGCAGCTCAACAGAGGCATGGTGCACGCAGACCG
  GCACAGCCACTCGCTGAGGAACCTCAACATTGGCAGTTCCTATGCGTACAGCCGGCCCGACGCCTTGGTC
  TACAGCCAGCCCGAGATTCGGGAGCACCCTCACCTCACCTCTCCCCAGTCAGCCCACTACCCCTTTAACC
  TGAACTACAGTTTCCACAGCCAGTCTCCGTATCCCTACCCTGCCGAGAGGCGGCCAGTGGTGGGCGCCGT
  GAGTGTGCCTGAACTGACAAATGTGCAGCTGCAGGCTCAGGACTACCCAGCTCCAAACATTATGAGAACC
  CAGGTGTACCGCCCACCCCCGCCGTATCCTTACCCAAGACCTGCCAACAGCACCCCAGACCTGTCCCGCC
  ACCTCTACATCAGCAGCAGCAATCCGGATCTCATCACCAGGCGCGTCCATCACTCAGTGCAGACCTTCCA
  GGAGGACAGCTTGCCCGTGGCTCACTCTCTGCAGGAGGTCAGCGAGCCCCTCACAGCAGCGCGCCATGCC
  CACCTACAGAAGAGGAACAGTATTGAAATCGCAGGGCTCACACATGGATTCGAAGGCCTGAGGCTCAAGG
  AGAGGACGGTGTCAGCCTCAGCAGCAGACGTGGCGCCCCGGACCTTCTCAGCAGGCTCCCAGTCCAGCGT
  CTTCTCTGACAAAATGAAGCAGGAGGGGACTGAAGAGCAGGAGGGTGGCAGATACAGCCATAAGAAGTCC
  CTTTCTGATGCCACCATGCTGATTCATAGCAGTGAGGAGGACGAGGACTTGGAAGAGGACAGCAGCAGAG
  AACAGGCCATCTCAGCTGTGTCTGAGCCCCGCCTTACGGCCGCTTTCTCTCAGGAACTAAACTACCCCTG
  TGCTTCAGCGACTCCGATCACTGGGCCTCTGCATATTTTTGAGCCCAAGCCCCATGTCACAGAGCCTGAG
  AAGAGGGCAAAGGACATCAGCCCTGCCCACCTGGTTGTGGAGACCCATCGGCCCCGAAGAGATGGACTGC
  TGACCCCCTCCATGTCCGAGTCAGACCTCACGACCTCAGGGAGGTACCGAGCTAGGAGGGACTCTGTCAA
  GAAAAGGCCAGTGTCAGACCTCCTCTCTGGGAAAAAAAGCGCTGTGGAAGGACTTCCGCCACTAGGGGGG
  ATGAAAAAAACTCGAGCAGATGCAAAAAAAATTGGTCCCCTCAAGCTGGCAGCCCTCAATGGGCTCTCCC
  TGTCCCGGCTGCCCCTGCCTGATGAAGGAAAAGAAGTGTCCACCAGAGCGACAAATGACGAGAGGTGCAA
  GGTTCTGGAGCAGCGGTTAGAGCAAGGCATGGTCTTCACCGAATATGAGAGGATCCTTAAGAAGCGGCTG
  GTTGATGGTGAGTGCTCGACTGCCCGGCTCCCTGAAAATGCAGAGAGAAATCGATTTCAAGATGTGCTCC
  CCTACGATGATGCGAGAGTCGAGTTGGTGCCAACCAAGGAGAACAACACTGGCTACATCAACGCGTCACA
  CATTAAGGTCTCTGTCAGCGGAATAGAATGGGATTATATTGCCACACAGGGACCATTACAAAATACCTGC
  CAGGACTTTTGGCAGATGGTGTGGGAACAGGGAGTCGCAATTATAGCAATGGTGACAGCAGAAGAGGAGG
  GAGGCCGGGAGAAGAGCTTTAGGTACTGGCCACGGCTTGGTTCCAGGCACAACACTGTCACCTATGGAAG
  GTTTAAGATCACAACGCGCTTCCGCACGGACTCGGGCTGCTATGCCACCACAGGTTTGAAGATGAAGCAC
  CTTCTCACAGGGCAGGAGAGAACCGTTTGGCATCTTCAGTACACAGACTGGCCTGAACACGGCTGTCCCG
  AAGACCTCAAAGGATTTTTATCCTACCTTGAAGAGATCCAGTCAGTTCGACGTCATACAAACAGTACCAG
  TGAACCCAAAAGCCACAATCCCCCGCTGCTGGTGCACTGCAGTGCTGGCGTGGGAAGAACTGGGGTCGTC
  ATTCTGTCAGAGATCATGGTTGCCTGTCTGGAACACAATGAGGTGCTGGACATCCCCAGAGTGCTGGACA
  TGCTGAGGCAGCAGAGGATGATGCTGGTGCAGACACTTGGCCAGTACACCTTTGTGTACAGAGTGCTCAT
  TCAGTTCCTGAAAAGCTCCAGGCTCATT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216090 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPLPFGLKLKRTRRYTVSSKSCLVARIQLLNNEFVEFTLSVESTGQESLEAVAQRLELREVTYFSLWYYN
  KQNQRRWVDLEKPLKKQLDKHALEPTVYFGVLFYVPSVSQLQQEITRYQYYLQLKKDILEGNLPCTLEHA
  IQLAGLAVQADFGDFDQYESQDFLQKFALLPVAWLQDEKVLEEAAQKVALLHQKYRGLTAPEAELLYMQE
  VERMDGYGEESYPAKDSQGSDISIGACLDGIFVKHKNGRPPVVFRWHDIANMSHNKSFFALELANKEETI
  QFQTEDMETAKYVWRLCVARHKFYRLNQCSLQTQAATLNSVRRGSSSRMSLPKPQPYAMPPPPQLHYNGH
  YTEPFASSQDNIFVPNKNGFYCHSQTSLDRTQIDLSGRIRNGSVYSAHSTNSLNTLQPYLQPSPMSSNPS
  ITGSDVMRPDYIPSHRHSALIPPSYRPTPDYETVMKQLNRGMVHADRHSHSLRNLNIGSSYAYSRPDALV
  YSQPEIREHPHLTSPQSAHYPFNLNYSFHSQSPYPYPAERRPVVGAVSVPELTNVQLQAQDYPAPNIMRT
  QVYRPPPPYPYPRPANSTPDLSRHLYISSSNPDLITRRVHHSVQTFQEDSLPVAHSLQEVSEPLTAARHA
  HLQKRNSIEIAGLTHGFEGLRLKERTVSASAADVAPRTFSAGSQSSVFSDKMKQEGTEEQEGGRYSHKKS
  LSDATMLIHSSEEDEDLEEDSSREQAISAVSEPRLTAAFSQELNYPCASATPITGPLHIFEPKPHVTEPE
  KRAKDISPAHLVVETHRPRRDGLLTPSMSESDLTTSGRYRARRDSVKKRPVSDLLSGKKSAVEGLPPLGG
  MKKTRADAKKIGPLKLAALNGLSLSRLPLPDEGKEVSTRATNDERCKVLEQRLEQGMVFTEYERILKKRL
  VDGECSTARLPENAERNRFQDVLPYDDARVELVPTKENNTGYINASHIKVSVSGIEWDYIATQGPLQNTC
  QDFWQMVWEQGVAIIAMVTAEEEGGREKSFRYWPRLGSRHNTVTYGRFKITTRFRTDSGCYATTGLKMKH
  LLTGQERTVWHLQYTDWPEHGCPEDLKGFLSYLEEIQSVRRHTNSTSEPKSHNPPLLVHCSAGVGRTGVV
  ILSEIMVACLEHNEVLDIPRVLDMLRQQRMMLVQTLGQYTFVYRVLIQFLKSSRLI
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001146199
• ORF Size: 3531 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001146199.1, NP_001139671.1
• RefSeq Size: 5562 bp
• RefSeq ORF: 3531 bp
• Locus ID: 24000
• Cytogenetics: 12 E
• MW: 133.5 kDa