Reps1 (NM_009048) Mouse Tagged ORF Clone

CAT#: MR216050

Reps1 (Myc-DDK-tagged) - Mouse RalBP1 associated Eps domain containing protein (Reps1), transcript variant 1

Product Images

Circular map for MR216050

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Reps1
• Synonyms: BB161292
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR216050 representing NM_009048
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGCTTGACGCTGAGCGACGCCGAGCAGAAATACTACTCGGATCTCTTCTCCTACTGCGACATCG
  AGAGCACCAAGAAGGTGGTGGTCAACGGGCGAGTGCTCGAGCTGTTCCGGGCCGCGCAGCTGCCCAACGA
  CGTGGTCCTCCAGATCATGGAGCTTTGTGGTGCAACAAGACTTGGTTATTTTGGAAGAAGTCAGTTCTAC
  ATTGCTTTGAAGCTTGTAGCTGTTGCCCAGTCTGGTTTTCCATTAAGAGTGGAAAGTATAAACACTGTAA
  AGGATCTCCCTCTCCCACGATTTGTTGCTTCAAAGAATGAACAGGAATCTCGCCTTGCAGCCTCCTATTC
  TTCAGATTCTGAAAACCAGGGCTCTTACTCGGGTGTGATCCCCCCTCCTCCCGGCAGAGGCCAAGTCAAG
  AAGGGGCCTGGAAGCCATGACGCGGTTCAGCCTCGTCCGTCTGCAGAGCAGCAGGAACCTGTGTCTCCAG
  TAGTTTCACCCCAGCAGTCCCCACCAACTTCTCCCCACACATGGAGGAAGCACAGCCGCCATCCCAGTGG
  GGGGAATAGTGAGAGACCTCTTACAGGACCGGGGCCATTCTGGTCTCCTTTTGGTGATGCACAAGCAGGC
  TCCTCTGCTGGTGATGCGGTGTGGTCTGGACAGTCCCCGCCTCCACCTCAAGACAACTGGGTCAGTTTTG
  CAGATACTCCACCAACCAGTGCTCTTTTAACCATGCATCCTGCTTCTGTCCAGGACCAGACGACAGTACG
  GACTGTAGCCTCAGCTGCAACTGCCAATGAGATCCGTAGGCAATCCAGTAGTTATGAGGATCCCTGGAAG
  ATAACAGATGAGCAGAGACAGTATTATGTAAATCAGTTTAAGACCATTCAGCCTGACCTAAACGGATTTA
  TCCCAGGATCTGCAGCTAAAGAGTTTTTTACAAAATCAAAACTTCCTATTCTTGAACTTTCTCATATCTG
  GGAACTCTCAGACTTTGATAAAGATGGCGCATTGACACTGGATGAGTTTTGTGCTGCTTTTCATCTGGTA
  GTTGCTAGAAAAAATGGCTATGACCTACCAGAGAAACTCCCTGAAAGCCTGATGCCCAAGCTGATAGACT
  TGGAAGATTCGGCAGATGTGGGGGAGCAGCCAGGTGAGGTAGGTTACTCAGGCTCTCCTGCAGAAGCACC
  TCCAAGCAAGTCACCGTCGATGCCGTCCCTGAACCAGACATGGCCAGAGCTGAATCAGAGCAGTGAGCAG
  TGGGAGACATTTAGTGAACGCTCTTCAAGCTCACAAACTCTGACCCAATTTGATTCTAACATTGCACCAG
  CTGATCCTGATACCGCTATTGTGCATCCAGTCCCCATCCGCATGACTCCAAGCAAAATCCACATGCAGGA
  AATGGAGCTGAAGAGAACAAGCAGCGATCACACTAATCCCACCAGCCCGTTGCTTGTGAAACCATCTGAC
  CTTTCAGAAGAAAATAAAATCAATTCATCAGTAAAATTTCCTTCTGGAAATACCGTAGATGGGTACAGTA
  GCTCAGACTCTTTCCCCTCTGACCCGGAGCAGATTGGGAGCAGTGTAACTCGTCAAAGGTCTCATTCAGG
  AACGTCACCTGATAACACTGCGCCACCACCTCCCCCTCCCAGGCCACAGCCTTCTCATTCTAGATCATCA
  TCTTTAGATATGAATCGGACCTTTGCAGTTACCACAGGACAACAACAGGCTGGAGTTGTTGCCCATCCTC
  CTGCAGTGCCTCCAAGACCTCAGCCCTCACAGGCACCCGGTCCTTCTGTCCATCGCCCAGTGGATGCAGA
  TGGTCTAATAACTCACACTAGTACCTCACCTCAGCAGATACCAGAACAACCAAATTTTGCAGATTTCAGC
  CAATTTGAAGTATTTGCTGCATCAAATGTCTCCGAAGAACAAGACAGTGAAGCTGAGAAACATCCTGAGG
  TCTTGCCAGCTGAGAAAGCTTCTGACCCCTCAAGCTCTCTTCGGGCTGCCCAAGCGGACAGTAAAGCTGA
  AGAAAAGACAGCTACTAATGTTCCTGCCAATGTGAGCAAAGGCACAACACCTCTGGCTCCACCACCAAAA
  CCTGTTAGAAGAAGATTAAAGTCAGAAGATGAGTTACGGCCAGATGTGGATGAGCATACGCAGAAGACAG
  GCGTCCTAGCTGCTGTTCTAACATCACAGCCTTCTATTCCCAGATCTGTGGGGAAAGATAAAAAGGCTAT
  CCAGGCATCCATTAGACGAAATAAGGAAACCAACACGGTTCTGGCCAGGCTGAACAGTGAATTGCAACAG
  CAATTAAAGGATGTTCTGGAGGAGAGAATCTCCCTGGAAGTTCAACTGGAGCAGCTCCGACCGTTTTCTC
  ACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR216050 representing NM_009048
  Red=Cloning site Green=Tags(s)
  
  MEGLTLSDAEQKYYSDLFSYCDIESTKKVVVNGRVLELFRAAQLPNDVVLQIMELCGATRLGYFGRSQFY
  IALKLVAVAQSGFPLRVESINTVKDLPLPRFVASKNEQESRLAASYSSDSENQGSYSGVIPPPPGRGQVK
  KGPGSHDAVQPRPSAEQQEPVSPVVSPQQSPPTSPHTWRKHSRHPSGGNSERPLTGPGPFWSPFGDAQAG
  SSAGDAVWSGQSPPPPQDNWVSFADTPPTSALLTMHPASVQDQTTVRTVASAATANEIRRQSSSYEDPWK
  ITDEQRQYYVNQFKTIQPDLNGFIPGSAAKEFFTKSKLPILELSHIWELSDFDKDGALTLDEFCAAFHLV
  VARKNGYDLPEKLPESLMPKLIDLEDSADVGEQPGEVGYSGSPAEAPPSKSPSMPSLNQTWPELNQSSEQ
  WETFSERSSSSQTLTQFDSNIAPADPDTAIVHPVPIRMTPSKIHMQEMELKRTSSDHTNPTSPLLVKPSD
  LSEENKINSSVKFPSGNTVDGYSSSDSFPSDPEQIGSSVTRQRSHSGTSPDNTAPPPPPPRPQPSHSRSS
  SLDMNRTFAVTTGQQQAGVVAHPPAVPPRPQPSQAPGPSVHRPVDADGLITHTSTSPQQIPEQPNFADFS
  QFEVFAASNVSEEQDSEAEKHPEVLPAEKASDPSSSLRAAQADSKAEEKTATNVPANVSKGTTPLAPPPK
  PVRRRLKSEDELRPDVDEHTQKTGVLAAVLTSQPSIPRSVGKDKKAIQASIRRNKETNTVLARLNSELQQ
  QLKDVLEERISLEVQLEQLRPFSHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009048
• ORF Size: 2385 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009048.2, NP_033074.2
• RefSeq Size: 2618 bp
• RefSeq ORF: 2388 bp
• Locus ID: 19707
• UniProt ID: O54916
• Cytogenetics: 10 A3
• MW: 87 kDa
• Gene Summary: May coordinate the cellular actions of activated EGF receptors and Ral-GTPases.[UniProtKB/Swiss-Prot Function]