Fip1l1 (NM_001159573) Mouse Tagged ORF Clone

CAT#: MR215977

Fip1l1 (Myc-DDK-tagged) - Mouse FIP1 like 1 (S. cerevisiae) (Fip1l1), transcript variant 1

Product Images

Circular map for MR215977

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fip1l1
• Synonyms: 1300019H17Rik; Rje
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215977 representing NM_001159573
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCGGCGAGGTGGAGCGCCTGGTGGAGCTGAGCGGCGGGACCGGAGGGGATGAGGAGGAAGAGT
  GGCTGTATGGCGGCCCGTGGGACGTGCATGTGCACAGTGACTTGGCAAAGGATCTAGATGAGAATGAAGT
  TGAAAGGCCAGAAGAAGAAAATGCCAGTGCTAATCCTCCATCTGGAATTGAAGAAGAAGCTGCTGAAAAT
  GGCGTGGCAAAACCGAAAGTGACAGAGACTGAAGACGACAGTGATAGTGACAGTGACGATGATGAAGATG
  ATGTTCACGTCACTATAGGAGACATCAAAACAGGAGCACCACAGTATGGGAGTTATGGAACAGCACCAGT
  AAATCTTAATATCAAGGCAGGGGGAAGAGTTTATGGAAATACAGGAACCAAAGTCAAAGGAGTGGACCTC
  GATGCACCTGGCAGCATTAATGGAGTTCCACTCTTGGAAGTAGATCTGGATTCTTTTGAAGATAAACCAT
  GGCGGAAACCTGGTGCTGATCTTTCTGATTATTTTAACTATGGCTTTAATGAAGATACTTGGAAAGCTTA
  CTGTGAAAAACAAAAGAGGATACGAATGGGACTGGAAGTTATACCTGTTACTTCAACCACAAACAAGATT
  ACGGTACAGCAAGGGAGAACTGGAAATTCAGAGAAAGAAGCAGCACTTCCATCTACAAAAGCTGAGTTTA
  CTTCTCCGCCATCATTGTTCAAGACTGGTCTGCCACCAAGCAGAAACAGCACCTCTTCTCAGTCTCAGAC
  AAGTACTGCCTCCAGAAAAGCCAGTTCAAGCGTTGGGAAGTGGCAGGATCGATATGGGAGGGCCGAGTCA
  CCTGATCTAAGGAGATTACCTGGAGCAATTGATGTCATTGGTCAAACAATAACCATCAGCCGAGTGGAAG
  GAAGACGGCGTGCAAATGAGAACAGCAATATACAGGTCCTTTCTGACAGATCTGCCACTGAAGTAGACAA
  CAATTTCAGCAAACCACCTCCATTTTTCCCTCCAGGGGCTCCTCCTACTCACCTTCCTCCTCCTCCATTT
  CTCCCACCACCTCCAACTGTCAGCACCGCCCCACCTCTCATTCCACCACCAGGTATTCCAATAACTGTAC
  CACCTCCAGGTTTCCCCCCTCCACCAGGCGCCCCACCTCCATCTCTTATACCAACAATAGAAAGTGGCCA
  TTCCTCCGGGTATGACAGCCGTTCTGCACGAGCGTTTCCCTATGGCAATGTCGCCTTTCCCCACCTTACC
  AGTTCTGCTCCCTCATGGCCGAGCCTTGTGGACACCACCAAGCAGTGGGACTATTATGCAAGGAGAGAAA
  AAGACCGAGACCGAGACCGGGAGAGAGACCGAGACAGAGAGCGAGAGCGGGACCGGGACAGAGAAAGGGA
  GCGCACCCGGGAGCGGGAGCGAGAGCGCGACCACAGCCCCACCCCAAGTGTTTTCAACAGTGACGAAGAG
  CGATACAGATACAGGGAGTATGCAGAGAGAGGCTATGAGCGCCATAGAGCTAGTCGGGAGAAAGAAGAGC
  GGCACAGAGAACGACGTCACAGAGAGAAGGAGGAAACAAGACACAAGTCCTCTCGCAGTAATAGTAGACG
  TCGCCATGAAAGTGAAGAAGGGGACAGTCACAGAAGACACAAGCACAAAAAGTCTAAAAGAAGCAAAGAA
  GGAAAAGAGGCTGGCAGCGAGCCGGTCCCAGAACAGGAGAGCACCGAAGCCGCCCCTGCCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215977 representing NM_001159573
  Red=Cloning site Green=Tags(s)
  
  MSAGEVERLVELSGGTGGDEEEEWLYGGPWDVHVHSDLAKDLDENEVERPEEENASANPPSGIEEEAAEN
  GVAKPKVTETEDDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAPVNLNIKAGGRVYGNTGTKVKGVDL
  DAPGSINGVPLLEVDLDSFEDKPWRKPGADLSDYFNYGFNEDTWKAYCEKQKRIRMGLEVIPVTSTTNKI
  TVQQGRTGNSEKEAALPSTKAEFTSPPSLFKTGLPPSRNSTSSQSQTSTASRKASSSVGKWQDRYGRAES
  PDLRRLPGAIDVIGQTITISRVEGRRRANENSNIQVLSDRSATEVDNNFSKPPPFFPPGAPPTHLPPPPF
  LPPPPTVSTAPPLIPPPGIPITVPPPGFPPPPGAPPPSLIPTIESGHSSGYDSRSARAFPYGNVAFPHLT
  SSAPSWPSLVDTTKQWDYYARREKDRDRDRERDRDRERERDRDRERERTRERERERDHSPTPSVFNSDEE
  RYRYREYAERGYERHRASREKEERHRERRHREKEETRHKSSRSNSRRRHESEEGDSHRRHKHKKSKRSKE
  GKEAGSEPVPEQESTEAAPAE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159573
• ORF Size: 1743 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159573.2
• RefSeq Size: 3082 bp
• RefSeq ORF: 1746 bp
• Locus ID: 66899
• UniProt ID: Q9D824
• Cytogenetics: 5 C3.3
• MW: 65.4 kDa
• Gene Summary: Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. FIP1L1 contributes to poly(A) site recognition and stimulates poly(A) addition. Binds to U-rich RNA sequence elements surrounding the poly(A) site. May act to tether poly(A) polymerase to the CPSF complex (By similarity).[UniProtKB/Swiss-Prot Function]