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Emc1 (NM_001039200) Mouse Tagged ORF Clone

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SKU
MR215902
Emc1 (Myc-DDK-tagged) - Mouse RIKEN cDNA C230096C10 gene (C230096C10Rik), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $910.00
5 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Emc1 or NM_001039200"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Emc1
Synonyms 2700016F22Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR215902 representing NM_001039200
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTCGCGGTGGCGTCTGGGTTCTGGATCTGGGCTGCTGTGCTGCTCGTCCCTGCGGCCGCGGTCT
ACGAAGACCAAGTGGGCAAGTTTGACTGGAGACAGCAGTATGTTGGGAAGATCAAGTTTGCCTCCTTGGA
GTTTTCCCCTGGATCCAAGAAGTTGGTTGTGGCCACAGAGAAGAATGTGATTGCGGCGTTAAATTCTCGG
ACTGGAGAGATCTTGTGGCGCCATGTTGACAAAGGCACAGCAGAAGGAGCTGTGGATGCCATGCTGGTCC
ATGGACAAGATGCAATCACTGTATCCAACGGAGGGCGCCTCATGCGTTCCTGGGAGACCAACATCGGGGG
CCTGAACTGGGAGATAACTCTGGACACTGGCAGTTTCCAGGCACTTGGGCTAGTGGGCCTTCAGGAGTCA
GTGAGGTACATTGCAGTTCTGAAGAAGACGACCCTCACCCTCCACCACCTCTCCAGTGGGCACCTGAAGT
GGGTGGAGCATCTGCCAGAAAGTGACAGCATCCTCTACCAGATGGTGTATTCCTACGGCTCCGGGGTGGT
GTGGGCCCTTGGCATCGTTCCCTTCAGCCACGTGAACATCGTCAAGTTTAACGTGGAGGATGGAGAGATT
GTTCAGCAGGTCAGGGTTTGGACTCCATGGCTGCAGCACCTCACTGGGGCCTGTGGTGTGGTAGATGAGG
CTGTCCTGGTATGCCCCGACCCCAGCTCACACTCACTCCACACTTTGGCCCTGGAGACGGAGTGGGAACT
GCGACAGATCCCCCTGCAGTCTCCTGACTTAGAATTTGGAAGTGGATTCCAGCCCCAAGTGCTGCCCACA
CAACCCAGCCCAGTGGCTCCTTCTCGAGCCCAGTTCTTCCTGCAGTTGTCCCCAAGCCACTACGCTCTAC
TGCACTACCACCATGGTGCCGTGACTCTGCTTAAGAACTTCCCCCAGGCCACGCTCGTCAGCTTCGCCAC
CACAGGGGAGAAGACAGTGGCTGCAGTCATGACCTGTCGCACTGAAGTGCAAAAACCTGTCAGTGCTGGA
GATGGGTCTGTGGCAAGCTTTCCTGAGACATCTGGTGCACAGGACTCTCTGGCTTGCTTCAACCAGACCT
ACACCATTAACCTGTACTTAGTGGAGACAGGTCGGCGGCTCCTAGACACTTCCATTTCCTTCAGCCTGGA
GCAGAAGGGCACGCGACCTGAGCAGCTGTACATCCAGGTGTTCTTGAAGAAGGACGACTCGGTGGGCTAC
CGGGCCTTGGTGCAGACACAGGACCATCTGCAGCTGTTCCTGCAGCAGCTTGCTGGGAAGGTGGTGTTGT
GGAGCCGAGAGGAGTCCCTGGCAGAAGTCGTGTGCCTGGAGATGGTGGATCTCCCCCTGACTGGAGCGCA
GGCTGAGTTGGAAGGAGAATTTGGCAAGAAGGCAGATGGCTTGCTGGGGATGTTCCTGAAACGCCTGTCG
TCCCAGCTCATCCTGCTCCAGGCCTGGACTTCCCACCTCTGGAAAATGTTCTACGATGCTCGGAAGCCCC
GAAGCCAGATTAAGAATGAGATCAACATCGACACACTGGCTAGAGATGAGTTCAACCTGCAGAAAATGAT
GGTGACGGTAACAGCCTCAGGCAAGCTCTTTGGCATCGAGAGCAGCTCTGGAACCATTCTGTGGAAGCAG
TATCTGCCCAATGTTAAGCCAGACTCCTCCTTTAAGCTGATGGTGCAGAGGACTACTGCCCATTTCCCCC
ATCCACCCCAGTGCACGCTGCTGGTGAAGGACAAGGAGACAGGAATGAGCTCTCTGTTTGTCTTCAACCC
CATTTTTGGCAAGTGGAGCCAGGTAGCTCCCCCAGTGCTGAAGCGCCCCATCTTGCAGTCACTGCTTCTC
CCAGTCATGGATCAAGACTATGCCAAGGTGTTGCTGTTGGTGGATGACGAATACAAGGTCACAGCTTTCC
CAGCTACGCGGAATGTCTTGCGGCAGCTGCATGAACTTGCCCCCTCCATCTTCTTCTACTTGGTGGATGC
AGAGCAGGGGCGGCTCAGTGGGTATCAGCTTCGAAAGGATCTCACTACAGAACTGAGTTGGGAGCTGACC
ATCCCCCCAGAAGTGCAGCGCGTCGTCAAGGTCAAGGGGAAGCGCAGCAGCGAGCACGTACACTCCCAGG
GCCGGGTGATGGGGGATCGAAGTGTGCTCTACAAGAGTCTGAACCCCAACTTGCTGGCCGTGGTGACAGA
GAGCACGGATGTCCACCACGAGCGCACCTTTATTGGCATCTTCCTCATTGATGGTGTCACTGGCCGCATC
ATCCACTCCTCCGTGCAGAAGAAGGCCAGGGGCCCTGTCCACCTTGTGCATTCAGAGAACTGGGTGGTGT
ACCAGTACTGGAATTCTAAGGCTCGGCGCAATGAGCTGACGGCCCTGGAGCTCTACGAGGGCACTGAACA
GTACAATGCCACTGCCTTCAGCTCTCTAGACCGACCCCAGCTGCCCCAGGTCCTCCAGCAGTCCTACATC
TTCCCCTCCTCCATCAGTGCCATGGAAGCTACCATCACCGAGAGGGGCATCACCAGCCGGCACTTGCTCA
TTGGGCTGCCTTCTGGAGCAATCCTTTCCCTCCCCAAGGCCTTGCTGGATCCCCGGCGCCCAGAGATCCC
AACAGAGCAAAGCAGAGAAGAGAACCTGATCCCATATTCTCCAGATGTTCAGGTCCATGCAGAGCGATTC
ATCAACTATAACCAGACAGTCTCTCGAATGCGAGGCATCTATACAGCGCCCTCAGGCCTGGAGTCCACTT
GTTTGGTTGTGGCCTACGGTTTGGATATTTACCAAACTCGAGTTTACCCGTCCAAGCAGTTTGATGTCCT
GAAAGATGACTACGACTATGTGCTCATCAGCAGTGTCCTTTTTGGCCTGGTTTTTGCCACCATGATCACA
AAGAGGCTGGCACAGGTGAAACTCCTCAATCGAGCCTGGCGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR215902 representing NM_001039200
Red=Cloning site Green=Tags(s)

MAVAVASGFWIWAAVLLVPAAAVYEDQVGKFDWRQQYVGKIKFASLEFSPGSKKLVVATEKNVIAALNSR
TGEILWRHVDKGTAEGAVDAMLVHGQDAITVSNGGRLMRSWETNIGGLNWEITLDTGSFQALGLVGLQES
VRYIAVLKKTTLTLHHLSSGHLKWVEHLPESDSILYQMVYSYGSGVVWALGIVPFSHVNIVKFNVEDGEI
VQQVRVWTPWLQHLTGACGVVDEAVLVCPDPSSHSLHTLALETEWELRQIPLQSPDLEFGSGFQPQVLPT
QPSPVAPSRAQFFLQLSPSHYALLHYHHGAVTLLKNFPQATLVSFATTGEKTVAAVMTCRTEVQKPVSAG
DGSVASFPETSGAQDSLACFNQTYTINLYLVETGRRLLDTSISFSLEQKGTRPEQLYIQVFLKKDDSVGY
RALVQTQDHLQLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLS
SQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVTVTASGKLFGIESSSGTILWKQ
YLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKETGMSSLFVFNPIFGKWSQVAPPVLKRPILQSLLL
PVMDQDYAKVLLLVDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLSGYQLRKDLTTELSWELT
IPPEVQRVVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGIFLIDGVTGRI
IHSSVQKKARGPVHLVHSENWVVYQYWNSKARRNELTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYI
FPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQVHAERF
INYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMIT
KRLAQVKLLNRAWR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001039200
ORF Size 2982 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001039200.2, NP_001034289.1
RefSeq Size 6217 bp
RefSeq ORF 2985 bp
Locus ID 230866
UniProt ID Q8C7X2
Cytogenetics 4 D3
MW 111.7 kDa
Summary Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001039200" in other vectors (4)
SKU Description Size Price
MC223009 Emc1 (untagged) - Mouse RIKEN cDNA C230096C10 gene (C230096C10Rik), transcript variant 2, (10ug) 10 ug
$1,002.00
MG215902 Emc1 (tGFP-tagged) - Mouse RIKEN cDNA C230096C10 gene (C230096C10Rik) transcript variant 2, (10ug) 10 ug
$789.00 $1,110.00
MR215902L3 Lenti ORF clone of Emc1 (Myc-DDK-tagged) - Mouse RIKEN cDNA C230096C10 gene (C230096C10Rik), transcript variant 2 10 ug
$1,210.00
MR215902L4 Lenti ORF clone of Emc1 (mGFP-tagged) - Mouse RIKEN cDNA C230096C10 gene (C230096C10Rik), transcript variant 2 10 ug
$1,210.00

file-alt Citations

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