Rad21 (NM_009009) Mouse Tagged ORF Clone

CAT#: MR215893

Rad21 (Myc-DDK-tagged) - Mouse RAD21 homolog (S. pombe) (Rad21)

Product Images

Circular map for MR215893

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rad21
• Synonyms: mKIAA0078; SCC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215893 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTACGCACATTTTGTCCTCAGTAAACGAGGGCCTCTGGCCAAAATCTGGCTGGCGGCCCATTGGG
  ACAAGAAGCTAACCAAAGCCCATGTATTTGAGTGCAACTTAGAGAGCAGTGTGGAGAGCATCATCTCACC
  AAAGGTGAAGATGGCGCTGCGGACGTCAGGACACCTTCTCCTGGGAGTAGTCCGCATCTATCACAGGAAA
  GCCAAATACCTCCTCGCAGACTGTAATGAAGCATTTATTAAAATAAAGATGGCGTTTCGGCCAGGTGTTG
  TCGATCTACCTGAGGAAAATCGGGAAGCAGCTTATAATGCCATTACTTTACCTGAGGAATTCCACGATTT
  TGATCAGCCACTGCCAGATTTAGATGATATTGACGTCGCCCAGCAGTTCAGCCTGAACCAAAGCAGAGTA
  GAAGAGATAACCATGAGAGAAGAAGTCGGAAACATCAGTATCCTACAGGAAAATGACTTTGGTGACTTCG
  GAATGGATGACCGTGAAATAATGAGAGAAGGCAGTGCTTTCGAGGATGACGACATGTTAGTGAGCACCAG
  CGCTTCCAACCTTCTCCTCGAGCCAGAGCAGAGCACCAGCAACCTGAATGAAAAGATGAATCACTTAGAG
  TACGAAGACCAGTACAAAGATGACAATTTTGGAGAAGGAAATGATGGCGGTATATTAGATGACAAACTTA
  TAAGTAATAATGATGGTGGCATCTTTGACGATCCCCCTGCCTTGTCTGAGGCAGGGGTCATGTTGCCAGA
  GCAACCTGCACATGATGACATGGATGAAGATGACAATGGCTCACTGGGTGGGCCGGATAGTCCCGACTCT
  GTGGATCCTGTCGAACCGATGCCAACTATGACTGATCAGACAACTCTCGTCCCAAACGAGGAAGAAGCTT
  TTGCGTTGGAGCCCATTGATATAACTGTCAAAGAGACAAAAGCCAAGAGGAAGAGGAAGCTGATTGTTGA
  CAGTGTCAAAGAATTGGATAGTAAGACCATTAGAGCCCAGCTTAGCGATTATTCTGATATTGTTACGACT
  CTGGACCTGGCTCCGCCAACCAAGAAGCTTATGATGTGGAAAGAGACAGGAGGAGTGGAAAAGCTCTTCT
  TTTTACCAGCACAGCCCCTGTGGAATAACCGGCTACTGAAGCTCTTCACACGCTGCCTTACCCCACTTGT
  ACCAGAAGACCTTAGGAAGAGAAGGAAAGGGGGAGAGGCAGATAATCTGGATGAGTTCCTCAAAGAGTTT
  GAGAATCCAGAGGTTCCCAGAGAGGAGCAGCAGCCACAGCAGCAGCAGCCACAGCCGCAGCGAGATGTCA
  TCGATGAGCCCATTATAGAAGAGCCAAGCCGCCTCCAGGACTCAGTGATGGAGGCCAGCAGAACAACCAT
  AGAAGAATCAGCCATGCCCCCACCACCCCCTCAAGGAGTTAAGCGGAAAGCCGGGCAAATAGACCCAGAG
  CCTTCGATACCTCCTCAGCAGGTAGAGCAAATGGAAATACCACCAGTAGAACTTCCCCCAGAGGAGCCTC
  CAAATATCTGTCAGCTGATCCCGGAGTTAGAGCTCCTACCGGAGAAGGAGAAGGAAAAAGAGAAGGAGAA
  GGAAGAGGAGGAGGAGGAGGAGGATGAAGATGCTTCAGGGGGTGATCAGGATCAAGAGGAAAGGAGATGG
  AACAAACGCACTCAGCAGATGCTTCATGGTCTTCAGCGAGCTCTTGCTAAAACTGGAGCAGAGTCTATCA
  GTTTGCTTGAGCTGTGTCGAAACACAAACCGAAAGCAGGCAGCAGCAAAGTTCTACAGCTTTTTGGTTCT
  TAAGAAGCAGCAAGCCATCGAGCTCACACAGGAAGAGCCGTACAGTGACATCATTGCAACCCCTGGACCA
  CGGTTCCATATTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215893 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFYAHFVLSKRGPLAKIWLAAHWDKKLTKAHVFECNLESSVESIISPKVKMALRTSGHLLLGVVRIYHRK
  AKYLLADCNEAFIKIKMAFRPGVVDLPEENREAAYNAITLPEEFHDFDQPLPDLDDIDVAQQFSLNQSRV
  EEITMREEVGNISILQENDFGDFGMDDREIMREGSAFEDDDMLVSTSASNLLLEPEQSTSNLNEKMNHLE
  YEDQYKDDNFGEGNDGGILDDKLISNNDGGIFDDPPALSEAGVMLPEQPAHDDMDEDDNGSLGGPDSPDS
  VDPVEPMPTMTDQTTLVPNEEEAFALEPIDITVKETKAKRKRKLIVDSVKELDSKTIRAQLSDYSDIVTT
  LDLAPPTKKLMMWKETGGVEKLFFLPAQPLWNNRLLKLFTRCLTPLVPEDLRKRRKGGEADNLDEFLKEF
  ENPEVPREEQQPQQQQPQPQRDVIDEPIIEEPSRLQDSVMEASRTTIEESAMPPPPPQGVKRKAGQIDPE
  PSIPPQQVEQMEIPPVELPPEEPPNICQLIPELELLPEKEKEKEKEKEEEEEEEDEDASGGDQDQEERRW
  NKRTQQMLHGLQRALAKTGAESISLLELCRNTNRKQAAAKFYSFLVLKKQQAIELTQEEPYSDIIATPGP
  RFHII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009009
• ORF Size: 1908 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009009.4, NP_033035.3
• RefSeq Size: 3632 bp
• RefSeq ORF: 1908 bp
• Locus ID: 19357
• UniProt ID: Q61550
• Cytogenetics: 15 C
• MW: 72.1 kDa
• Gene Summary: Double-strand-break repair protein rad21 homolog: As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions. The cohesin complex may also play a role in spindle pole assembly during mitosis (By similarity). In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (PubMed:18237772). May control RUNX1 gene expression. Binds to and represses APOB gene promoter (By similarity). May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).[UniProtKB/Swiss-Prot Function]