Dcaf6 (NM_028759) Mouse Tagged ORF Clone

CAT#: MR215880

Dcaf6 (Myc-DDK-tagged) - Mouse DDB1 and CUL4 associated factor 6 (Dcaf6)

Product Images

Circular map for MR215880

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dcaf6
• Synonyms: 1200006M05Rik; Iqwd1; PC326
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215880 representing NM_028759
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGGAGTGGCTCCTGCCCGCACCTGTTGTGGGACGTGAGGAAAAGGTCCCTTGGGCTGGAGGACC
  CGTCCCGGCTGAGGAGCCGCTACCTGGGAAGAAGAGAATTTATCCAAAGATTAAAACTTGAAGCAACTTT
  AAATGTGCATGATGGCTGTGTTAATACAATCTGTTGGAATGACACTGGAGAATATATTTTATCTGGCTCT
  GATGACACTAAACTTGTAATTAGTAATCCATACAGCAGAAAGGTTTTGACAACCATCCGTTCAGGGCATC
  GAGCAAATATATTTAGTGCAAAGTTTTTGCCGTGCACAGATGATAAGCAGATTGTGTCTTGCTCTGGAGA
  TGGAGTCATATTTTATACTAACATTGAGCAAGATGCAGAAACTAACAGACAGTGCCAATTTACGTGCCAT
  TATGGAACTACTTATGAGATTATGACTGTACCAAACGACCCTTACACCTTTCTGTCCTGTGGTGAAGATG
  GAACTGTTAGGTGGTTTGACACACGCATCAAAACCAGTTGCACAAAAGAAGACTGTAAAGATGATATTTT
  AATCAACTGTAGGCGTGCTGCCACATCTGTGGCTATTTGTCCCCCAGTACCATATTACCTTGCTGTGGGT
  TGTTCTGACAGCTCAGTACGGATTTATGATCGGCGAATGCTGGGCACAAGAGCTACAGGGAATTATGCAG
  GCCGAGGAACTACTGGAATGGTTGCTCGATTTATACCTTCTCATCTTAGTAACAAATCATGCAGAGTGAC
  ATCACTGTGTTACAGTGAAGATGGTCAAGAGATTCTTGTCAGTTATTCTTCAGACTACATCTATCTTTTT
  GACCCCAAAGATGATACTGCACGAGAACTTAAAACTCCTTCTGCAGAGGAGAGGCGAGAAGAGTTACGAC
  AGCCTCCAGTTAAGCGCTTGAGACTTCGTGGTGATTGGTCAGATACTGGTCCCAGAGCACGGCCAGAAAG
  TGAACGAGAACGAGATGGAGAGCAAAGTCCCAATGTGTCACTGATGCAGAGAATGTCTGATATGTTATCA
  AGGTGGTTTGAAGAAGCAAGTGAAGTTGCACAAAGCAACAGAGGAAGAGGAAGACCTCGGCCCAGAGGTG
  GAACAAATCAGCCAGATGTTTCAACTCTTCCTACGGTTCCATCAAGTCCTAATTTGGAAGTGTGTGAAAC
  TGCAATGGATGTAGACATGCCAGCTGCACTTCTTCAGCCTTCTACATCCTCTACAGATCCAGTTCAGGCT
  CAGGCAGCCACAGCCGCCATAGAAAGCCCTCGTTCCAGCTCGTTGCTGTCTTGCCCAGACAGTGAACCGA
  GGCAGTCTGTTGAGGCGTCTGGACACCATGCACATCATCAGTCAGATAACAGTAATGAGAGGCTGAGCCC
  CAAACCAGGGACAGGTGAACCCGTTTTAAGTTTGCACTACAGCACAGAAGGAACAACTACAAGCACAATA
  AAACTGAACTTTACAGATGAATGGAGCAGTACAGCCTCAAGTTCCAGAGGAAATGGGAGCCATTGCAAAT
  CTGAGGGTCAGGAAGAATGCTTGGTCCCTCCGAGCTCTGTGCAGCCACCGGAAGGAGACAGTGAAACAAG
  AGCTCCTGAAGAACTATCAGAGAAAGGAACACTTCCAGAAAACCTCACTCAAAACCAGATAGATACAGCA
  CAACTTGATAACTTCCCAGCTGAGCCATTGGATTCTAACTCAGGAGAGAAGAATAACCCAAGTCAGGACA
  GCCCTTGTGGGCTTCCAGAAGAAGGCACTTTGTCTGAAACAGACAGGGAGACTTGTGAGCAGGCCAGCAC
  TGAGAGTGCTACCAGGCATGCTAGCACCAAGCCTGAACTCCCATCCCAGACAGAAGCCATTGAGCAGGCC
  AGCACTGAGAGTGCTACCAGGCATACCAGTGCCAATCCTGAACTCCCATCCCAGACAGAAGCCATAGCAC
  CTTTAGCTCATGAAGACCCATCTGCCAGGGACTCTGCTCTCCAGGACACAGATGACAGCGATGATGATCC
  GGTCTTGATCCCTGGTGCAAGATACCGAACAGGACCTGGTGATAGACGCTCCGCTGTTGCCCGCATTCAG
  GAGTTCTTCAGGAGGAGAAAAGAAAGGAAAGAAATGGAAGAGCTGGATACTTTGAACATTAGGAGGCCAC
  TAGTAAAGATGGTTTATAAGGGCCACCGCAACTCCCGGACAATGATAAAAGAAGCCAATTTCTGGGGTGC
  TAACTTTGTAATGAGCGGTTCCGATTGTGGCCATATCTTCATCTGGGACCGGCACACTGCTGAGCATTTG
  ATGCTTCTGGAAGCTGATAATCATGTGGTCAACTGCCTGCAGCCCCATCCGTTTGACCCAATTCTAGCCT
  CATCTGGCATAGATTATGACATAAAGATCTGGTCGCCACTAGAAGAGTCAAGAATTTTTAATCGAAAACT
  TGCTGATGAAGTTATAACTCGGAATGAACTCATGCTGGAAGAGACTCGGAACACCATCACCGTCCCAGCC
  TCTTTCATGTTGAGGATGTTGGCGTCACTGAATCATATCCGAGCTGACCGTCTGGAGGGTGACAGATCAG
  AAGGTTCAGGTCAGGAGAATGAAAATGAGGATGAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215880 representing NM_028759
  Red=Cloning site Green=Tags(s)
  
  MARSGSCPHLLWDVRKRSLGLEDPSRLRSRYLGRREFIQRLKLEATLNVHDGCVNTICWNDTGEYILSGS
  DDTKLVISNPYSRKVLTTIRSGHRANIFSAKFLPCTDDKQIVSCSGDGVIFYTNIEQDAETNRQCQFTCH
  YGTTYEIMTVPNDPYTFLSCGEDGTVRWFDTRIKTSCTKEDCKDDILINCRRAATSVAICPPVPYYLAVG
  CSDSSVRIYDRRMLGTRATGNYAGRGTTGMVARFIPSHLSNKSCRVTSLCYSEDGQEILVSYSSDYIYLF
  DPKDDTARELKTPSAEERREELRQPPVKRLRLRGDWSDTGPRARPESERERDGEQSPNVSLMQRMSDMLS
  RWFEEASEVAQSNRGRGRPRPRGGTNQPDVSTLPTVPSSPNLEVCETAMDVDMPAALLQPSTSSTDPVQA
  QAATAAIESPRSSSLLSCPDSEPRQSVEASGHHAHHQSDNSNERLSPKPGTGEPVLSLHYSTEGTTTSTI
  KLNFTDEWSSTASSSRGNGSHCKSEGQEECLVPPSSVQPPEGDSETRAPEELSEKGTLPENLTQNQIDTA
  QLDNFPAEPLDSNSGEKNNPSQDSPCGLPEEGTLSETDRETCEQASTESATRHASTKPELPSQTEAIEQA
  STESATRHTSANPELPSQTEAIAPLAHEDPSARDSALQDTDDSDDDPVLIPGARYRTGPGDRRSAVARIQ
  EFFRRRKERKEMEELDTLNIRRPLVKMVYKGHRNSRTMIKEANFWGANFVMSGSDCGHIFIWDRHTAEHL
  MLLEADNHVVNCLQPHPFDPILASSGIDYDIKIWSPLEESRIFNRKLADEVITRNELMLEETRNTITVPA
  SFMLRMLASLNHIRADRLEGDRSEGSGQENENEDEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028759
• ORF Size: 2628 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028759.1, NP_083035.1
• RefSeq Size: 3306 bp
• RefSeq ORF: 2631 bp
• Locus ID: 74106
• UniProt ID: Q9DC22
• Cytogenetics: 1 H2.2- H2.3
• MW: 97.6 kDa
• Gene Summary: Ligand-dependent coactivator of nuclear receptors. Enhance transcriptional activity of the nuclear receptors NR3C1 and AR. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex (By similarity).[UniProtKB/Swiss-Prot Function]