Cul2 (NM_029402) Mouse Tagged ORF Clone

CAT#: MR215691

Cul2 (Myc-DDK-tagged) - Mouse cullin 2 (Cul2)

Product Images

Circular map for MR215691

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cul2
• Synonyms: 1300003D18Rik; 4932411N15Rik; AI327301; mKIAA4106
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215691 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTAAAACCAAGAGTAGTGGATTTTGATGAGACATGGAACAAACTCCTAACTACAATCAAAGCTG
  TTGTTATGTTGGAATATGTCGAGCGAGCAACATGGAATGACCGCTTCTCAGATATCTATGCTTTATGTGT
  GGCCTATCCTGAACCACTTGGAGAAAGACTTTATGCGGAAACAAAGATTTTTTTGGAAAGTCACGTTCGG
  CATTTGTATAAGAGAGTTCTGGAGTCTGAAGAACAAGTGCTTGTTATGTATCATAGGTACTGGGAAGAAT
  ACAGCAAAGGCGCCGACTATATGGACTGCTTATATAGGTATCTCAATACCCAGTATATTAAAAAGAATAA
  ATTGACAGAAGCTGATATACAGTATGGCTATGGTGGAGTAGATATGAATGAACCACTTATGGAAATAGGA
  GAGCTAGCATTGGATATGTGGAGGAAGTTAATGGTTGAACCCCTTCAGAACATCCTCATCCGGATGCTGC
  TTCGAGAAATTAAAAATGATCGTGGTGGTGAAGATCCAAACCAGAAAGTAATCCATGGGGTTATTAACTC
  CTTTGTTCATGTTGAACAGTATAAGAAAAAATTTCCCTTAAAGTTTTATCAGGGAATATTCGTGTCTCCC
  TTTCTGACTGAAACGGGAGAATATTACAAGCAAGAAGCTTCAAATTTATTACAAGAATCAAATTGTTCAC
  AGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATTAGATGTCGAAAATATTTGCATCCAAG
  TTCATATACTAAAGTGATTCATGAGTGTCAGCAGCGGATGGTAGCAGACCACTTACAGTTCTTACATTCA
  GAGTGCCACAGCATCATTCAGCAAGAGAGGAAGAACGACATGGCAAATATGTACGTCTTACTCCGTGCTG
  TGTCCAGTGGTTTACCTCATATGATTGAGGAGCTGCAGAAACATATCCATGACGAGGGCCTCAGAGCGAC
  CAGTAACCTTACTCAGGAACATATGCCAACACTATTTGTGGAATCAGTTTTGGAAGTACATGGTAAATTC
  GTTCAACTTATCAACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGCTTTAGATAAGGCCCTTACAT
  CTGTTGTAAATTACAGAGAACCCAAGTCTGTTTGCAAAGCGCCTGAACTGCTTGCTAAGTACTGTGACAA
  CTTGCTAAAGAAGTCAGCGAAGGGGATGACTGAAAATGAAGTGGAGGACAAGCTCACTAGCTTCATCACA
  GTTTTCAAGTACATTGATGACAAGGACGTCTTTCAGAAGTTCTATGCAAGAATGCTAGCAAAACGTCTAA
  TTCACGGGTTGTCTATGTCTATGGATTCTGAAGAAGCCATGATCAATAAATTAAAGCAAGCCTGTGGTTA
  TGAGTTTACCAGCAAGCTGCATCGAATGTATACAGACATGAGTGTCAGCGCTGATTTGAACAATAAGTTC
  AACAATTTCATCAGAAACCAAGACACCGTGATTGACTTGGGGATTAGTTTCCAAATATATGTTCTTCAGG
  CTGGTGCGTGGCCTCTCACTCAGGCTCCTTCATCTACATTTGCAATTCCCCAAGAGCTAGAGAAAAGTGT
  ACAGATGTTTGAATTGTTTTATAGCCAGCATTTCAGTGGACGGAAGCTGACATGGTTGCATTATCTCTGT
  ACAGGTGAAGTTAAAATGAACTATTTGGGAAAACCATATGTAGCTATGGTTACAACCTACCAAATGGCAG
  TTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGCTACAAAGAACTTCAAGACAGTACCCAGATGAATGA
  AAAGGAGCTGACCAAAACAATCAAATCATTACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAA
  GATATCGATGCAGAATCTTCATTTTCATTAAATATGAGCTTCAGCAGTAAGAGAACAAAATTTAAGATTA
  CTACGTCAATGCAGAAGGATACGCCACAAGAACTGGAACAGACTAGAAGTGCTGTAGATGAGGACCGGAA
  AATGTATCTTCAAGCTGCTATTGTCCGCATCATGAAAGCACGAAAAGTGCTTCGGCACAACGCCCTCATT
  CAAGAGGTAATCAGCCAGTCAAGAGCCAGGTTCAATCCCAGTATCAGCATGATTAAGAAGTGCATAGAAG
  TTCTGATAGACAAACAGTACATCGAGCGCAGCCAAGCATCTGCAGATGAATACAGTTACGTCGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215691 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYAETKIFLESHVR
  HLYKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQYIKKNKLTEADIQYGYGGVDMNEPLMEIG
  ELALDMWRKLMVEPLQNILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQGIFVSP
  FLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHS
  ECHSIIQQERKNDMANMYVLLRAVSSGLPHMIEELQKHIHDEGLRATSNLTQEHMPTLFVESVLEVHGKF
  VQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDKLTSFIT
  VFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKF
  NNFIRNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLC
  TGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKE
  DIDAESSFSLNMSFSSKRTKFKITTSMQKDTPQELEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALI
  QEVISQSRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029402
• ORF Size: 2235 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029402.3, NP_083678.2
• RefSeq Size: 4158 bp
• RefSeq ORF: 2238 bp
• Locus ID: 71745
• UniProt ID: Q9D4H8
• Cytogenetics: 18 A1
• MW: 86.9 kDa
• Gene Summary: Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (By similarity). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) (By similarity).[UniProtKB/Swiss-Prot Function]