Chchd6 (NM_001167736) Mouse Tagged ORF Clone

CAT#: MR215645

Chchd6 (Myc-DDK-tagged) - Mouse coiled-coil-helix-coiled-coil-helix domain containing 6 (Chchd6), transcript variant 2

Product Images

Circular map for MR215645

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Chchd6
• Synonyms: 0710001P09Rik; 1700021B03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215645 representing NM_001167736
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCGCCGAGAGCGCCGAGGCCCGAAGGGTGTCCTTCGAGATGGATGAGGAGGAGCGGGTCCGGG
  TGCTGCAGGGCATCCGGCTGTCTGAAAGTGTTGTGAACCGCATGAAGGATTGCAGCCAGCCTTCAGCAGG
  GGAACAGCTGGTTCCTGGTTTTGGTCCTTCGTCTTCTGCTCCTGTGCCCACTGTCCCTCTACCAGCCATC
  TCTGTGCCCACTGTCCCTGCACCTACCACTCCTGTGCCTACTGCTCCTTCATCCTCTGTACGTGGCCTGC
  CAGGAGGCACCTGTAAAGGCCCTCTGACAGACGTTAAAGTGCCCAGTGCAGAGAGTGGTGGTGGTCTGCA
  GTCCTCAGCAGTGAAGGAGGATCTTAAGAAGTTCCAACAGGAGCAGTTGGCAGTCCAGGATGAGATGGTC
  AGGGTGGCAAAAAAGGAAAAAGAGGCAGCTGAGAAGCACTTGAAGGCATCCCTGCCCAAGAAGAAGGCCA
  GCCTCACCCATGAGCAACAGCAGTCAGCTCGACTAAATGCTGAGCTGTATAAACTGTCTTCCCAACAATT
  CCATGAGGCGGCTTCAAAAGCAGAGAGCACAATAAAGCCCCGCAGAGTGGAGCCTGTGTGCTCTGGCCTA
  CAAGCCCAGATCCTCCGCTGTTACCGGGACCATCTGCATGAAGTGCTTCTGTGCTCGGACCTGGTCAAGG
  CATACCAGCACTGTGTGAGCACTGCCCGCAAGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215645 representing NM_001167736
  Red=Cloning site Green=Tags(s)
  
  MGSAESAEARRVSFEMDEEERVRVLQGIRLSESVVNRMKDCSQPSAGEQLVPGFGPSSSAPVPTVPLPAI
  SVPTVPAPTTPVPTAPSSSVRGLPGGTCKGPLTDVKVPSAESGGGLQSSAVKEDLKKFQQEQLAVQDEMV
  RVAKKEKEAAEKHLKASLPKKKASLTHEQQQSARLNAELYKLSSQQFHEAASKAESTIKPRRVEPVCSGL
  QAQILRCYRDHLHEVLLCSDLVKAYQHCVSTARKG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001167736
• ORF Size: 735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167736.1, NP_001161208.1
• RefSeq Size: 1042 bp
• RefSeq ORF: 738 bp
• Locus ID: 66098
• UniProt ID: Q91VN4
• Cytogenetics: 6 D1
• MW: 26.8 kDa
• Gene Summary: Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.[UniProtKB/Swiss-Prot Function]