Synpo (NM_177340) Mouse Tagged ORF Clone

CAT#: MR215490

Synpo (Myc-DDK-tagged) - Mouse synaptopodin (Synpo), transcript variant A

Product Images

Circular map for MR215490

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Synpo
• Synonyms: 9030217H17Rik; 9130229N11; 9330140I15Rik; AW046661
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215490 representing NM_177340
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGTACTCAGAGGAAGCCAGTCTGCTGCGGCATCTGGAGAAGGTTGCCAGTGAGGAAGAGGAAG
  TGCCATTGGTAGTTTATTTAAAGGAGAATGCAGCCCTGCTGACAGCCAATGGGCTACACCTGTCGCAGAG
  CCGAGAGACCCAGCAGTCCTCACCAAACCCTCCCGACACTGAAGTACCCAGTCCAGCTGCAGATATCAAC
  CAGAACCCGTCCTCACCTAATGCCACACTCACCACACTAGCCTCTAGCAGTCACCACAGTCAGCCCACCG
  CTGATATCAACCAAAACCCTCCAGCCGCCATCACCCCTGTCCCACAGAATTCATCCCAGGCACAGTGCTC
  CCCGAATGGCACACTTGATTCCAAACCCGGCACTCTGTGTGCTGATGATGGGCAGTCCCCGGTGCCGGCA
  GAGGAGGTGAGATCCAGCATTCTCTTGATTGACAAGGTGTCAGCTCCACCTTCTGCCGCCAGCACCTTCT
  CTAGAGAAGCCACGCCCCTCTCCAGCTCTGGGCCGCCAGCGGCAGATCTCATGTCCAGCTCCCTGCTCAT
  TGACATGCAGCCTAGCACCCTAGTGGCACCAGCAGAACAAGAGGTGCCTGGCCATGTAGCTGTCACCACG
  CCCACTAAGGTGTATAGTGAAGTACATCTCACACTAGCCAAGCCTGCATCCGTGGTCAACAGGACCGCCA
  GGCCTTTTGGGATCCAGTCGCCAGGGACCAGCCAGATAGAGCAAAGCCCCATGATGGGAAGACGACAGTT
  TGGAGAGAAGGCCTGGGCTCCTCCTGCTAGCAGTATGGCGGATAGGAGTCCCCAGCCACAGAGGCACATA
  ATGTCCCGCAGTCCCATGGTGGAAAGGAGACTGCTTGGGCAGCGAAGCCCGGTCCTAGAGAGACGCCCCT
  TAGGAAATTTCACCCCACCCCCCACCTATGCGGAGACTTTGTCAACAGCCCCTGTGGCTTCTCGGGTTAG
  GTCTCCCCCCTCTTACTCCACTCTGTATCCCAGCTCTGACCCCAAGCCTTCCCATTTGAAAGGTCAGGTA
  GCCCCTGCCAACAAGACAGGAATTTTGGAAGAATCTATGGCACGCCGAGGCAGCCGGAAATCAATGTTCA
  CTTTCGTGGAGAAGCCCAAGGTGACCCCGAATCCAGATTTGCTGGACCTAGTGCAGACAGCTGATGAGAA
  GCGGAGGCAGAGAGACCACGGGGAGGTGGGCATGGAAGAAGAGCCCTTTGCCCTGGGAGCTGAGGCCTCC
  AACTTCCAGCAGGAGCCAATAGCTCGGGACAGGGCCAGCCCTGCGGCAGCTGAGGAAGCTGTCCCTGAGT
  GGGCCTCTTGCCTCAAGTCACCCCGTATCCAGGCCAAGCCGAAGCCCAAACCCAATCAGAACCTCTCGGA
  AGCCTCAGGGAAGGGGGCTGAGCTCTATGCCCGCCGCCAGTCACGGATGGAGAAATACGTCATAGAGTCT
  TCAGGCCATGCCGAATTGGCCCGCTGCCCTTCACCTACCATGTCCCTGCCTTCATCCTGGAAGTACACCA
  CTAACGCCCCTGGGGGCTTCCGAGTGGCATCCTTAAGTCCAGCGCGGACCCCGCCTGCCTCTCTCTACCA
  CGGCTATCTGCCAGAGAATGGAGTCCTGCGCCCTGAACCTACCAAGCAGCAGCCATACCAGATGCGGCCT
  TCCCTCTATGCTCTGTCGCCCGTCAAGGAACCTGCCAAGGCCTCATCGCGTGCCACCTCATCGCGCACTC
  CATCACGCACTGTCTCACCTCGAGCTGCCTCCCCGGCCAAGCCTAGCTCTCTGGACCTGGTGCCCAACCT
  GCCCAGAGCAGGCCTCCCACCATCTCCTGCTCTGCCTCGGCCTTCCCGCTCCTCCCCAGGCCTCTACACT
  GCCCCAGTCCAAGACAGCCTCCAGCCCACTGCCGTGAGTCCCACTTACAGCAGTGATATCTCCCCCGTGT
  CTCCCTCCAGGGCGTGGTCTCCTCGAGCCAAGCAGGCTCCCAGGCCTTCCTTCTCCACCCGGAATGCTGG
  GATCGAGGCCCAGGACCGCCCGGAGAGCCTGCCCACGTCCCCGCCCTGGACGCCGGCCGCTTCCCGGCCT
  CCTAGCAGCCTGGACGGCTGGGTGAGCCCGGGGCCGTGGGAGCCGGGCCGCGGGAGCAGCATGAGCAGCC
  CTCCGCCGCTGCCGCCGCCTCCACCAATGTCCCCCTCGTGGAGCGAGCGCTCCGTCTCCCCGCTGCGATC
  GGAGACCGAGGCGCGGCCCCCGAGCCGCCAGCTGCAGGCGCTTCTGGCGCGCAACATCATCAACGCGGCC
  CGGCGCAAGAGCGCCTCCCCGCGGCCAGCGCCGGCTGAGACCCTGCGGCCCTTTTCCCCGCCGCAGGGGC
  CGCCGCCGCCGCCGGCGCGCATGCGCTCTCCGCAGCCAGCCAGCCCTGCCCGTAACTTCCGTGGAGCTGC
  TTTCTCTCCCATCCCGCGGAGCCCGTTGCCCATCGGACCTTCTTCCTGTGCTAGTCCCCGGAGTCCTCAA
  GCCGCGCCGTCCAGGCCTTTTCCCTACCGCCGCTCACCCACAGACTCCGACGTGTCTCTCGACTCCGAGG
  ATTCTGGGCTTAAGTCGCCTGGCATCCTCGGCTACAACATCTGTCCTCGGGGCTGGAATGGCAGCCTGAG
  GCTCAAGCGTGGTAGTCTCCCCACCGAGGCCTCCTGCACCACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215490 representing NM_177340
  Red=Cloning site Green=Tags(s)
  
  MEGYSEEASLLRHLEKVASEEEEVPLVVYLKENAALLTANGLHLSQSRETQQSSPNPPDTEVPSPAADIN
  QNPSSPNATLTTLASSSHHSQPTADINQNPPAAITPVPQNSSQAQCSPNGTLDSKPGTLCADDGQSPVPA
  EEVRSSILLIDKVSAPPSAASTFSREATPLSSSGPPAADLMSSSLLIDMQPSTLVAPAEQEVPGHVAVTT
  PTKVYSEVHLTLAKPASVVNRTARPFGIQSPGTSQIEQSPMMGRRQFGEKAWAPPASSMADRSPQPQRHI
  MSRSPMVERRLLGQRSPVLERRPLGNFTPPPTYAETLSTAPVASRVRSPPSYSTLYPSSDPKPSHLKGQV
  APANKTGILEESMARRGSRKSMFTFVEKPKVTPNPDLLDLVQTADEKRRQRDHGEVGMEEEPFALGAEAS
  NFQQEPIARDRASPAAAEEAVPEWASCLKSPRIQAKPKPKPNQNLSEASGKGAELYARRQSRMEKYVIES
  SGHAELARCPSPTMSLPSSWKYTTNAPGGFRVASLSPARTPPASLYHGYLPENGVLRPEPTKQQPYQMRP
  SLYALSPVKEPAKASSRATSSRTPSRTVSPRAASPAKPSSLDLVPNLPRAGLPPSPALPRPSRSSPGLYT
  APVQDSLQPTAVSPTYSSDISPVSPSRAWSPRAKQAPRPSFSTRNAGIEAQDRPESLPTSPPWTPAASRP
  PSSLDGWVSPGPWEPGRGSSMSSPPPLPPPPPMSPSWSERSVSPLRSETEARPPSRQLQALLARNIINAA
  RRKSASPRPAPAETLRPFSPPQGPPPPPARMRSPQPASPARNFRGAAFSPIPRSPLPIGPSSCASPRSPQ
  AAPSRPFPYRRSPTDSDVSLDSEDSGLKSPGILGYNICPRGWNGSLRLKRGSLPTEASCTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177340
• ORF Size: 2706 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177340.2, NP_796314.2
• RefSeq Size: 5012 bp
• RefSeq ORF: 2706 bp
• Locus ID: 104027
• Cytogenetics: 18 D3
• MW: 96.3 kDa