Sec14l1 (NM_001166506) Mouse Tagged ORF Clone

CAT#: MR215313

Sec14l1 (Myc-DDK-tagged) - Mouse SEC14-like 1 (S. cerevisiae) (Sec14l1), transcript variant 2

Product Images

Circular map for MR215313

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sec14l1
• Synonyms: 1200017E04Rik; 2810012L19Rik; Naa-35
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215313 representing NM_001166506
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAAAAATACCAGTCCCCAGTGCGGGTGTATAAACATCCCTTTGAGTTAATTATGGCTGCCTATG
  AGAGGAGGTTTCCTACATGTCCTCTGATTCCAATGTTCGTGGACAGTGACACTGTGAGCGAGTTCAAGAG
  TGAGGATGGGGCTCTTCATGTCATCGAGAGGCGCTGCAAGCTGGATATAGATGCGCCCAGGCTGTTGAAG
  AAGATCGCAGGAGTCGATTACGTTTATTTTGTCCAGAAAAACTCCCTGAATTCTCGAGATCGCACTTTGC
  ACATCGAGGCCCACAATGAGACATTTTCTAACCGGGTCATCATCCACGAACACTGCTGTTACACGGTTCA
  CCCTGAGAATGAAGACTGGACCTGTTTCGAACAGTCTGCAAGTTTAGATATCAAATCCTTCTTTGGTTTT
  GAAAGTACAGTGGAAAAAATTGCCATGAAACATTATACAAGCAACATTAAAAAAGGGAAGGAGATCATTG
  AATACTACCTACGGCAGCTGGAGGAGGAAGGCATCACCTTCGTGCCCCGCTGGACCCCACCCCCCGTGGG
  ACCCTCAGAAACATGTTCATCAAGCAAGAATCAAGTCACATCTGCAGCTGTCCTTGTCCCAGATGCTGCT
  GCCGTCATGGAGGGGCTGAGTGGGGAAAACCTCAGTAGCCCAGGAACAGCGTCTGAGCCCGTGGTGGGAA
  CCCCTGACGATAAATTAGATGCCGACTACATCAAGAGATACTTGGGTGACCTGACTCCCCTCCAAGAGAG
  CTGCCTCATTCGACTGCGCCAGTGGCTCCAGGAGACACACAAGGGCAAGATCCCAAAGGACGAGCATATC
  CTTCGGTTCCTGCGTGCCCGGGATTTTAATATTGACAAGGCCAGAGAGATCATGTGCCAGTCTCTAACCT
  GGCGGAAGCAGCACCAGGTGGATTACATCCTGGACACTTGGACTCCGCCCCAGGTCCTTCTGGACTACTA
  CGCTGGGGGCTGGCATCACCATGACAAAGATGGACGGCCGCTGTATGTGCTTAGGCTGGGCCAGATGGAC
  ACCAAAGGCTTGGTGCGAGCTCTCGGAGAGGAGGCCCTACTGCGATACGTTCTTTCCATAAACGAAGAAG
  GCCTGAGACGATGCGAGGAAAACACCAAGGTCTTCGGCCGGCCAATCAGCTCGTGGACCTGCTTGGTGGA
  CCTTGAAGGACTGAACATGCGCCATCTGTGGAGACCAGGGGTCAAAGCCTTGCTGCGCATCATCGAGGTG
  GTGGAGGCCAACTACCCGGAGACGCTGGGCCGCCTCCTCATCCTCCGAGCCCCCAGGGTCTTCCCTGTCC
  TCTGGACGCTGGTTAGTCCATTTATTGATGACAACACCAGAAGGAAGTTCCTCATTTATGCAGGAAATGA
  CTACCAGGGCCCAGGAGGCCTGCTGGATTACATCGATAAAGAGATTATTCCCGATTTCCTGAGTGGGGAG
  TGCATGTGTGACGTGCCAGAGGGTGGATTGGTCCCCAAATCCTTGTACAGGACTGCAGAGGAGCTGGAAA
  ATGAAGACCTGAAGCTCTGGACGGAGACCATCTACCAGTCTGCCAGCGTGTTCAAAGGAGCTCCACATGA
  GATTCTCATTCAGATTGTGGACGCCTCCTCAGTGATCACCTGGGATTTTGATGTGTGCAAAGGGGACATC
  GTCTTTAACATTTATCACTCCAAGAGGTCTCCCCAGCCACCCAAGAAGGACTCGCTAGGGGCTCACAGCA
  TCACTTCCCCAGGAGGCAACAACGTGCAGCTGATAGACAAAGTCTGGCAGCTGGGCCGGGACTATAGCAT
  GGTGGAGTCTCCTCTAATCTGCAAAGAAGGAGAGAGCGTGCAGGGGTCCCATGTGACCAGGTGGCCAGGC
  TTCTACATCCTACAGTGGAAATTCCACACCATGCCAGCATGTGCTGCCACCAACCTACCCCGAGTGGACG
  ACGTGCTGGCCTCCCTGCAGGTCTCCTCCCACAAGTGCAAAGTCATGTACTACACAGAAGTGATCGGCTC
  TGAAGATTTCAGAGGCTCCATGACCAGCCTGGAATCTAGCCACAGCGGGTTCTCACAGCTCAGTGCCGCC
  ACCACCTCCTCCAGCCAGTCGCAATCCAGCTCCATGATTTCCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215313 representing NM_001166506
  Red=Cloning site Green=Tags(s)
  
  MVQKYQSPVRVYKHPFELIMAAYERRFPTCPLIPMFVDSDTVSEFKSEDGALHVIERRCKLDIDAPRLLK
  KIAGVDYVYFVQKNSLNSRDRTLHIEAHNETFSNRVIIHEHCCYTVHPENEDWTCFEQSASLDIKSFFGF
  ESTVEKIAMKHYTSNIKKGKEIIEYYLRQLEEEGITFVPRWTPPPVGPSETCSSSKNQVTSAAVLVPDAA
  AVMEGLSGENLSSPGTASEPVVGTPDDKLDADYIKRYLGDLTPLQESCLIRLRQWLQETHKGKIPKDEHI
  LRFLRARDFNIDKAREIMCQSLTWRKQHQVDYILDTWTPPQVLLDYYAGGWHHHDKDGRPLYVLRLGQMD
  TKGLVRALGEEALLRYVLSINEEGLRRCEENTKVFGRPISSWTCLVDLEGLNMRHLWRPGVKALLRIIEV
  VEANYPETLGRLLILRAPRVFPVLWTLVSPFIDDNTRRKFLIYAGNDYQGPGGLLDYIDKEIIPDFLSGE
  CMCDVPEGGLVPKSLYRTAEELENEDLKLWTETIYQSASVFKGAPHEILIQIVDASSVITWDFDVCKGDI
  VFNIYHSKRSPQPPKKDSLGAHSITSPGGNNVQLIDKVWQLGRDYSMVESPLICKEGESVQGSHVTRWPG
  FYILQWKFHTMPACAATNLPRVDDVLASLQVSSHKCKVMYYTEVIGSEDFRGSMTSLESSHSGFSQLSAA
  TTSSSQSQSSSMISR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001166506
• ORF Size: 2145 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166506.2
• RefSeq Size: 4736 bp
• RefSeq ORF: 2148 bp
• Locus ID: 74136
• UniProt ID: A8Y5H7
• Cytogenetics: 11 E2
• MW: 81.7 kDa
• Gene Summary: May play a role in innate immunity by inhibiting the antiviral RIG-I signaling pathway. In this pathway, functions as a negative regulator of DDX58/RIG-I, the cytoplasmic sensor of viral nucleic acids. Prevents the interaction of DDX58 with MAVS/IPS1, an important step in signal propagation. May also regulate the SLC18A3 and SLC5A7 cholinergic transporters.[UniProtKB/Swiss-Prot Function]