Syce2 (NM_001168246) Mouse Tagged ORF Clone

CAT#: MR214964

Syce2 (Myc-DDK-tagged) - Mouse synaptonemal complex central element protein 2 (Syce2), transcript variant 1

Product Images

Circular map for MR214964

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syce2
• Synonyms: 1700013H19Rik; AA407907; Cesc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR214964 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGCCACGGAAGCTGGCTGCGCTCGCAGGTGGCCGCGCCTCCCGTGGAGTTAAAGGACCAGGAGC
  CGCCGGCGATTGTGGAGAGCGGAGAGCATCGGCAGAGTGAGAACCACGAGGAGACGCCCGGCTCAGTGGC
  CCCGAGTGCCAGTTGCCAGCTGCCAGGACCCTTCTCCTCTCTGGACTCTAGCATTGAAACCCTGAAGAAG
  AAAGCCCAGGAACTGATTGAAAACATCAATGAAAGCAGGCAGAAGGACCATGCACTTATGACCAACTTCA
  GGGACAGCCTCAAGATGAAGGTCTCAGATCTGACAGAAAAGTTGGAGGAGAGGATGTACCAGGTGTACAG
  CCACCACAGCAAAATCATTCAGGAAAGACTACAAGAATTTACCCAGAAGATGGCAAAGATCAACCATCTG
  GAAATGGAGCTTAAACAAGTCTGCCAAACTGTGGAAACGGTGTACAAGGACCTATGTGTCCAGTCTGAGG
  TTCCCACTTGCGAAGAACAGAATTACAAAGATGGTGAATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR214964 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERHGSWLRSQVAAPPVELKDQEPPAIVESGEHRQSENHEETPGSVAPSASCQLPGPFSSLDSSIETLKK
  KAQELIENINESRQKDHALMTNFRDSLKMKVSDLTEKLEERMYQVYSHHSKIIQERLQEFTQKMAKINHL
  EMELKQVCQTVETVYKDLCVQSEVPTCEEQNYKDGEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168246
• ORF Size: 531 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168246.1, NP_001161718.1
• RefSeq Size: 929 bp
• RefSeq ORF: 534 bp
• Locus ID: 71846
• Cytogenetics: 8 C3
• MW: 20.3 kDa
• Gene Summary: Major component of the transverse central element of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Requires SYCP1 in order to be incorporated into the central element. May have a role in the synaptonemal complex assembly, stabilization and recombination.[UniProtKB/Swiss-Prot Function]