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Tmem215 (NM_001166009) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR214313
Tmem215 (Myc-DDK-tagged) - Mouse transmembrane protein 215 (Tmem215), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Tmem215 or NM_001166009"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem215
Synonyms A930001M12Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR214313 representing NM_001166009
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGCCTGATGACATAAATCCGAGGACTGGGCTGGTGGTGGCCCTGGTCAGTGTTTTCCTGGTCTTTG
GTTTCATGTTCACTGTCTCTGGCATGAAGGGGGAAACTCTGGGGAACATCCCTCTGTTGGCCATCGGGCC
AGCCATCTGCCTACCAGGCATCGCCGCCATTGCTCTGGCCAGGAAAACCGAAGGCTGCACCAAGTGGCCA
GAGAATGAGCTACTCTGGGTCCGCAAGTTACCCTGCTTCCGGAAGCCCAAGGACAAGGAAGTGGTGGAAC
TCCTGAGGACCCCATCAGACCTGGAGTCAGGCAAAGGGAGCTCGGATGAACTGGCTAAGAAGGCAGGCCT
CAGAGGCAAGCAGCTCCCACAGGGACCAGGTGAGGTGCCTATGGCCAGCTCCGTTACTACCCCCACTCCT
ACAGAGGAAGGAGAATGCCAGAGTCTAGTCCAGAGTGGGCGGCAGGAAGAGACATCCAGATACCTGGATG
GCTATTGCCCCTCGGCCAGTTCCCTCGCCTACAGTGCCTTGGATGCCAAGTGCTCAGCCTGGGACAGATC
TGACCGCCCTGAGCCCGAGGACAGCATCTTCTTTGTGCCCCAGGACAGTATCATCGTTTGCTCCTACAAG
CAAAACAGCCCCTATGACAGATACTGTTGCTACATCAACCAGAGCCAAGGCAGGTGGGACCACGAGACAA
TAGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR214313 representing NM_001166009
Red=Cloning site Green=Tags(s)

MRPDDINPRTGLVVALVSVFLVFGFMFTVSGMKGETLGNIPLLAIGPAICLPGIAAIALARKTEGCTKWP
ENELLWVRKLPCFRKPKDKEVVELLRTPSDLESGKGSSDELAKKAGLRGKQLPQGPGEVPMASSVTTPTP
TEEGECQSLVQSGRQEETSRYLDGYCPSASSLAYSALDAKCSAWDRSDRPEPEDSIFFVPQDSIIVCSYK
QNSPYDRYCCYINQSQGRWDHETIV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001166009
ORF Size 705 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001166009.1, NP_001159481.1
RefSeq Size 2276 bp
RefSeq ORF 708 bp
Locus ID 320500
UniProt ID A7E1Z1
Cytogenetics 4 A5
MW 26.3 kDa
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Documents
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"NM_001166009" in other vectors (4)
SKU Description Size Price
MC214470 Tmem215 (untagged) - Mouse transmembrane protein 215 (Tmem215), transcript variant 2, (10ug) 10 ug
$330.00
MG214313 Tmem215 (tGFP-tagged) - Mouse transmembrane protein 215 (Tmem215) transcript variant 2, (10ug) 10 ug
$500.00
MR214313L3 Lenti ORF clone of Tmem215 (Myc-DDK-tagged) - Mouse transmembrane protein 215 (Tmem215), transcript variant 2 10 ug
$600.00
MR214313L4 Lenti ORF clone of Tmem215 (mGFP-tagged) - Mouse transmembrane protein 215 (Tmem215), transcript variant 2 10 ug
$600.00

Plasmid Map for MR214313

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR214313
Circular map for MR214313

file-alt Citations

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