Tmem114 (NM_029070) Mouse Tagged ORF Clone

CAT#: MR213860

  • TrueORF®

Cldn26 (Myc-DDK-tagged) - Mouse claudin 26 (Cldn26)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_029070" in other vectors (4)


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Reconstitution Protocol

USD 300.00

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Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Tmem114
Synonyms 4930511J11Rik; Cldn26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR213860 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGTGCGCCTGGGCGCTCTGGCGGGAGCGGCGGCGCTGAGCGGGGCTCTGAGCTTCGTGCTGCTGG
CAGCAGCCATCGGCACCGACTTCTGGTACATCATCGACACCGAGAGGCTGGAAAGGAGCAGCCAGAGGAT
GAGGGACCAGGGACCGGCCAACCGCAGCCAGCAAGAGCCTCTGAGCTCCCACTCGGGACTCTGGAGAACT
TGCCGGGTCCAGAGCTCCTGCACGCCGTTGATGAACCCTTTCTGGCAGGAGAATGTGACGGTCAGCGACT
CCAGCAGACAGCTTCTCACTATGCACGGGACATTCGTGATCTTATTGCCACTCAGTCTGATCGTGATGGT
GTTTGGGGGGATGACCGGGTTCTTGAGCTTCCTCCTCCGAGCTCACCTCCTTCTGCTGCTCACCGGGATC
CTCTTTCTCTTTGGAGCCATGGTGACCCTCACTGGAATCAGCATCTACATTGCGTACTCAGCAGTTGCCT
TCCGAGAGGCAGTATGCCTCCTGGAGGAGAGGGCCCTGCTGGATCAGGTGGATATACGCTTTGGCTGGTC
TCTGGCCCTCGGCTGGATTAGCTTTGTATCTGAGCTGCTCACTGGGGTGGTCTTCCTGGCAGCGGCGCGT
GCGCTCAGCCTGAGCCAGAGGCAGGACCAAGCCATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR213860 protein sequence
Red=Cloning site Green=Tags(s)

MRVRLGALAGAAALSGALSFVLLAAAIGTDFWYIIDTERLERSSQRMRDQGPANRSQQEPLSSHSGLWRT
CRVQSSCTPLMNPFWQENVTVSDSSRQLLTMHGTFVILLPLSLIVMVFGGMTGFLSFLLRAHLLLLLTGI
LFLFGAMVTLTGISIYIAYSAVAFREAVCLLEERALLDQVDIRFGWSLALGWISFVSELLTGVVFLAAAR
ALSLSQRQDQAI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_029070
ORF Size 669 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_029070.2, NP_083346.1
RefSeq Size 1406 bp
RefSeq ORF 669 bp
Locus ID 74720
UniProt ID Q9D563
Cytogenetics 16 A1
MW 24.4 kDa

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