Ceacam5 (NM_028480) Mouse Tagged ORF Clone

CAT#: MR213593

Ceacam5 (Myc-DDK-tagged) - Mouse carcinoembryonic antigen-related cell adhesion molecule 5 (Ceacam5)

Product Images

Circular map for MR213593

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ceacam5
• Synonyms: 1600029H12Rik; Psg30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR213593 representing NM_028480
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCATCCTCTGTCCTTCCATGCAAGTGGTGTACCCACTTGCAGGGGCTTCTGCTCACAGCCTCCT
  TTTTAACCTGCTGTCACCTGCCTACCACTGCACAAATAACCATTGAATTAGAGCCACCCCAAGTGATTGA
  AGGAGAAAATGTTCTCATACGTGTCAACAATTTGACAGAGAATCTTATAACCTTAGCCTGGTTCAGAGGA
  ATGAGGATTAAGAGCCCTCAAATTGGACAATATACACCGGCCACTAAAGTTACTGTGCTGGGTCCTGGTC
  ACAGTGGTAGAGAAACTTTGTACAGCAATGGATCCCTGCAGATCTACAATGTCACCCAGGAGGACATAGG
  ATTCTACAGCCTACGAATCATAAATAAACATGCAGAAATTGTGTCAATAACATCCATATACCTCAACGTG
  TACTCATCTCTATGGACATGTGAGCACCCTTCTCCCCATGCCAAGCTCACCATTGAATCAGTGCCACCCG
  GCATTTCTGAAGGGGGAAGTGTTCTTCTACTGGTTAAAAATCTCCCCCAAAATCTTCTATCCCTTTTCTG
  GTACAAAGGAGTTATTGCAGTGAAGAAATTTGAGATTGCCAGACACATAAAAGCCACAAATTCAAGTGTT
  CCAGGGCCTGCACACACTGGTCGTGAGACAGTATTCAGCAATGGATCCCTTCTGCTACAGGAAGTTATGC
  AGAGTGATACTGGATTCTATACTCTACGAACTATGAGTACAGATCTGAAAGATGAAGTAGCACATGTGCA
  GCTGTACATGGACACCTATCTTTTGACTTGTTATCACCCTTTGCAAGTCAAGATCGAATCACTTCCTCAG
  AATGTTGCTGTAGGGAAAACTGTTCTTCTCTTAGTTCATAATTTGCCTGAAGACTTTCAAGCATTTTTCT
  GGTACAAATCAGCATATAGAAGAGATACATACAAAATTGCAGAATACAAGAGAGCCATGGATGCCACCAT
  TTTGGGGAGTGCATACAGTTCAAGAGAGTTTATATACAACAACGGATCCATGCTAATAATTGATGTCACT
  GAGGATGATGCAGGATATTTCCTGCTAGAAATTTTAAGAGAAGACTTAAAGATTGAAAAAGCCTACATTC
  AACTCCATGTAAATAGCTTTGTTTCAAACTCCAAGGACTCTGCCTCCACTGCAAGGCTCAGTATAGAATC
  AGTGCCACCGAGCATTGTTGAAGGGGGAAGTGTTCTTTTACTGGTTCACAATCTCCCAAAAAATCTTCAA
  TCCCTTTTCTGGTACAAAGGGATGATTGCAGAGAAGAAATCTGAGCTCATACAACACATAATAGCCACAA
  GTTCAAGCTTGCCAGGGCCTGCACACAGTGGTCGAGAAACAGTATACAACAATGGATCCCTGCTGCTCCA
  GAGGGTCATGCAAAATGATACTCAATTTTATACTCTACAAACTATGGATACAGATCTGAAATATGAAGTA
  GCACATGTTCAACTCCAGCTGGACACTTCCCTTTCTACTTGGTATCACCCTTTGCAAGTCAAGATCGAAT
  CACTTCCTCGGAATGTTGCTGTAGGAAAAAGTGTTCTTTTTCTGGTTCATAATTTTCCAGAAGTCTTTCG
  AGCATTTTCCTGGTATAAACCAGCGTATAAGTCACATACATCCAAAATTGTAGAATACCACAGATTCACG
  GATTCTGCCACCGTGGGGGCTGCATACAGAGGAATAGAGGTGATATTCACCAACGGATCCATGGTGATGA
  TTGATGTCACTGAGGATGATGCAGGGTTTTACATGCTAGAAATTTTAAGAGAAGACTTCAAAGTTGAAAA
  AGCCTATGTTCAACTCCATGTAAATAGCTTTGTTCCAAACTCAAAGGTCTCTGTCTCCACTGCAAGGCTC
  AGTATAGAATCAGTGCCCCCCAGCATTGTTGGAGGGGAAAGTGTTCTTCTACTGGTTCACAATCTTCCAA
  AAAATCTTCAATCTCTTTTCTGGTACAAAGGCGTGATTGCCGAGGAGAAATCTGAGCTCATCCAACACAT
  AATAGCCACAAGTTCAAGCTTGCCAGGGCCTGCACACAGTGGTCGAGAAACAGTGTACAGCAATGGATCC
  CTGCTGCTCCAGAGGGTCATGCAGAATGATACTGGATTCTATACTCTACTAACTATGAGTACAGATCTGA
  AAGATGAAATAGCACATGTGCAACTCCAGCTGGACACCTCTACTTGCTGTAGCCTTCTCACCTCTGACCA
  ACTCATAATTGTACCAGTGCCTCGGAATATTGCTGTAGGGAAAAGTGTTCTTCTCCTTGTTTGCAATGTG
  CCAAAGGATGTTCAGACAATTTTCTGGTACAAATCGGTGTATCGAACAGATATTTTTAAAATTGCAGAAT
  ATAGCAGATCCATGGAATCTACTATCTGGGGGCTTGCACACAGTGGAAAAGAGATGGTGTACACTAATGG
  ATCCCTTCTGATCCAGAATGTCACTGAGCATGATGCAGGATTGTACATGTTAGAAATTTTACATAAGGAC
  TACAAACTTGAGAGAGCACATGTGCAAGTCCATGTAAATAACCCTGTTTCATGGCCTTTTGTGCGAGTGA
  CTGACACCACAGTCAGAGTACAGAGCTCAGTAGTCTTCACTTGCTTCTCAGCTGACCCTGGTGTCTCCAT
  CCGTTGGCTCTTCAATAAGCAGAGTCTGCAGCTCACAGAGAGAATGACACTTTCCCCATCAAAGTGCCAA
  CTCAGCATAGATCCTGTCTGGAGGGAGGATGCTGGAAAGTATCAATGTGAGGTCTCCAACCCAGTCAGTT
  CAAAGTCCAGTCTCCCAGTCCGTCTGGCTGTGATAGAAGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR213593 representing NM_028480
  Red=Cloning site Green=Tags(s)
  
  MEASSVLPCKWCTHLQGLLLTASFLTCCHLPTTAQITIELEPPQVIEGENVLIRVNNLTENLITLAWFRG
  MRIKSPQIGQYTPATKVTVLGPGHSGRETLYSNGSLQIYNVTQEDIGFYSLRIINKHAEIVSITSIYLNV
  YSSLWTCEHPSPHAKLTIESVPPGISEGGSVLLLVKNLPQNLLSLFWYKGVIAVKKFEIARHIKATNSSV
  PGPAHTGRETVFSNGSLLLQEVMQSDTGFYTLRTMSTDLKDEVAHVQLYMDTYLLTCYHPLQVKIESLPQ
  NVAVGKTVLLLVHNLPEDFQAFFWYKSAYRRDTYKIAEYKRAMDATILGSAYSSREFIYNNGSMLIIDVT
  EDDAGYFLLEILREDLKIEKAYIQLHVNSFVSNSKDSASTARLSIESVPPSIVEGGSVLLLVHNLPKNLQ
  SLFWYKGMIAEKKSELIQHIIATSSSLPGPAHSGRETVYNNGSLLLQRVMQNDTQFYTLQTMDTDLKYEV
  AHVQLQLDTSLSTWYHPLQVKIESLPRNVAVGKSVLFLVHNFPEVFRAFSWYKPAYKSHTSKIVEYHRFT
  DSATVGAAYRGIEVIFTNGSMVMIDVTEDDAGFYMLEILREDFKVEKAYVQLHVNSFVPNSKVSVSTARL
  SIESVPPSIVGGESVLLLVHNLPKNLQSLFWYKGVIAEEKSELIQHIIATSSSLPGPAHSGRETVYSNGS
  LLLQRVMQNDTGFYTLLTMSTDLKDEIAHVQLQLDTSTCCSLLTSDQLIIVPVPRNIAVGKSVLLLVCNV
  PKDVQTIFWYKSVYRTDIFKIAEYSRSMESTIWGLAHSGKEMVYTNGSLLIQNVTEHDAGLYMLEILHKD
  YKLERAHVQVHVNNPVSWPFVRVTDTTVRVQSSVVFTCFSADPGVSIRWLFNKQSLQLTERMTLSPSKCQ
  LSIDPVWREDAGKYQCEVSNPVSSKSSLPVRLAVIEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028480
• ORF Size: 2841 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028480.2, NP_082756.1
• RefSeq Size: 3248 bp
• RefSeq ORF: 2844 bp
• Locus ID: 73250
• UniProt ID: Q3UKK2
• Cytogenetics: 7 A2
• MW: 106.3 kDa
• Gene Summary: Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells.[UniProtKB/Swiss-Prot Function]