Mybbp1a (NM_016776) Mouse Tagged ORF Clone

CAT#: MR211937

Mybbp1a (Myc-DDK-tagged) - Mouse MYB binding protein (P160) 1a (Mybbp1a)

Product Images

Circular map for MR211937

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mybbp1a
• Synonyms: AL024407; AU019902; p67MBP; P160; p160MBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211937 representing NM_016776
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGATGAAGAGCCCCACGAAAGCTGAGCCTGCGACTCCCGCAGAAGCGGCGCAAAGCGACCGCC
  ACAGCCTGCTGGAGCACAGCCGCGAGTTCTTGGACTTCTTCTGGGACATTGCGAAACCGGATCAGGAAAC
  GCGGCTCCGGGCCACGGAGAAGTTGTTGGAGTACTTGCGCACAAGGCCCAATGATTCGGAGATGAAATAT
  GCCCTGAAGCGCCTAATCACTGGGCTTGGGGTGGGCCGAGAAGCCGCTAGGCCCTGCTACAGCCTGGCGC
  TGGCACAGCTGTTGCAGTCTTTTGAAGACATCCCATTGTGTGACATCCTGGATCAGATACAAGAAAAATA
  CAGCCTACAAGCCATGAACAAGGCAATGATGAGACCTAGTCTCTTTGCAAACCTTTTTGGAGTGCTAGCC
  CTCTTTCAGTCAGGCCGCCTAGTGAAGGACAAAGAGGCCCTGATGAAGTCCGTGCAATTGCTGAAGATCC
  TGTCCCAACACCCCAACCACTTACAGGGACAGCCAATAAAGGCTCTGGTGGACATCCTCTCTGAGGTCCC
  AGAGTCCATGTTCCAGGAGATCCTGCCAAAGGTCCTCAAGGGTAACATGAAAGTGATCCTCCGCTCTCCC
  AAGTACTTGGAGCTCTTCCTCCTGGCTAAGCAGAGGGTGCCGACAAAGCTCGAGTCACTCATGGGCTCGG
  TTGACCTGTTCTCAGAAGACAATATTCCCAGTCTGGTGAACATCCTGAAGGTGGCCGCCAACTCTGTCAA
  GAAGGAGCACAAGCTGCCTAATGTGGCTCTGGACCTGCTCCGCCTGGCGCTCAAGGAGAGCAGATTCGAA
  CTGTTCTGGAAGAAGGTTTTGGAGGAGGGGCTGCTGAAGAATCCGTCCTGGACATCCAGCTACATGTGCT
  TCCGCCTACTGGGTGCGTCTCTGCCGCTTCTGTCAGAGGAGCAGTTGCAGTTGGTGATGCGAGGAGACTT
  GATCCGCCATTTTGGGGAGAACATGGTTATTTCTAAGCCCCAAAACCTATTTAAGATCATCCCAGAGATA
  AGTACATACGTGGGTACCTTCCTAGAGGGGTGCCAGGATGACCCTAAGCGGCAGTTGACTATGATGGTGG
  CCTTTACAACCATCACCAATCAAGGTCTCCCTGTCATGCCTACCTTCTGGCGTGTCACGCGGTTTTTGAA
  TGCTGAAGCCCTGCAGAGCTATGTGGCCTGGTTGCGGGACATGTTCCTGCAGCCTGACCTGAACTCCTTG
  GTTGACTTCAGCACTGCCAACCAGAAGAGAGCTCAGGACGCCTCGTTGAATGTGCCTGAGCGAGCTGTAT
  TCCGGCTCCGGAAGTGGATCATCCACCGCCTGGTCAGCCTTGTGGATCATTTGCATCTGGAGAAGGATGA
  AGCTGTGGTTGAGCAAATAGCCAGGTTTTGCTTGTTCCATGCCTTCTTTAAGACGAAGAAGGCTACGCCC
  CAGATCCCAGAGACGAAGCAGCACTTCTCCTTCCCTTTGGACGACCGCAACCGTGGGGTCTTTGTCAGTG
  CCTTCTTCAGCCTACTGCAGACGCTCAGTGTGAAGTTCAGGCAGACACCAGACCTGGCTGAAAATGGGAA
  GCCTTGGACTTACCGCCTGGTTCAGTTGGCAGACATGCTGTTGAACCATAACCGCAATGTAACCAGTGTG
  ACATCCTTGACAACACAGCAGCGTCAGGCCTGGGACCAGATGATGAGTACTCTGAAGGAATTAGAGGCCC
  GCTCCTCTGAGACCAGGGCCATTGCCTTCCAGCACCTGCTGCTTCTGGTGGGCCTCCACATCTTCAAGTC
  CCCTGCAGAAAGCTGTGATGTCCTAGGAGACATTCAGACTTGCATCAAGAAAAGCATGGAGCAGAATCCC
  CGCCGATCACGCTCTAGAGCCAAAGCCTCCCAGGAGCCAGTATGGGTGGAGGTGATGGTGGAGATCTTGC
  TGTCCTTGCTGGCTCAACCCAGCAACTTGATGCGCCAGGTGGTCCGGAGTGTATTTGGTCATATCTGTCC
  CCACCTTACTCCACGTTGTCTGCAGCTAATCCTGGCTGTGCTCAGCCCTGTGACAAACGAGGATGAGGAT
  GACAACGTGGTGGTCACTGATGACGCTGATGAGAAGCAGCTGCAGCATGGAGAGGACGAAGACTCAGATA
  ATGAGGACAATAAGAACTCAGAGAGTGACATGGACAGTGAGGATGGGGAAGAAAGTGAAGAGGAGGACCG
  TGACAAAGATGTGGACCCAGGCTTCCGTCAACAGTTGATGGAAGTGTTAAAAGCTGGGAATGCATTGGGT
  GGAGTGGACAACGAGGAGGAGGAGGAGCTTGGGGATGAGGCCATGATGGCCCTGGACCAGAACCTGGCCA
  GCCTATTTAAAGAGCAGAAGATGCGCATCCAGGCCCGGAATGAGGAGAAAAACAAGCTACAGAAGGAGAA
  GAAGCTCCGACGGGACTTCCAAATCAGGGCACTAGACCTGATCGAGGTGCTGGTGACCAAGCAGCCTGAG
  CACCCCCTGATCCTGGAACTACTTGAGCCACTGCTGAACGTGATCCAGCACAGCATGCGCAGCAAAGGCT
  CCACCAAGCAGGAGCAGGACCTCCTGCACAAGACCGCCCGCATCTTCATGCACCACCTGTGTCGTGCCCG
  CCGCTACTGCCACGAGGTGGGGCCGTGTGCAGAGGCTCTGCATGCCCAGGTGGAGAGGCTTGTGCAGCAG
  GCTGGCAGCCAGGCTGATGCCTCTGTCGCCCTATACTATTTCAATGCCTCTCTGTACCTGCTGCGAGTCC
  TCAAGGGCAACACCAATAAGAGGCACCAAGATGGTCATAAGTTACACGGAGCTGACACAGAGGACTCAGA
  GGACCAGGCTGCTAACTGCTTAGACTTGGACTTTGTGACCCGGGTGTATTCAGCATCACTGGAATCTCTT
  CTGACCAAGCGTAACAGCTCACTTACGGTCCCCATGTTCCTCAGCCTCTTCTCCAGATACCCAGTGATCT
  GTAAGAACCTGCTTCCCGTCCTGGCTCAGCATGTGGCTGGCCCATCTCGGCCCCGCCATCAGGCCCAGGC
  ATGCCTGATGCTCCAGAAGACTCTGTCTGCACGAGAGCTGAGAGTCTGTTTTGAGGATCCTGAGTGGGAG
  CAGCTGATTACCCAACTCTTGGGAAAGGCCACCCAGACCCTGCAGACTCTTGGGGAGGCACAGAGCAAGG
  GGGAGCACCAGAAGGAGCTATCCATCTTGGAGCTGCTGAACACTCTGTTGAGGACAGTCAATCACGAGAA
  GCTGTCTGTGGACCTCACTGCTCCCTTGGGCGTGCTTCAGAGCAAGCAACAGAAGCTGCAGCAAAGCCTG
  CAGCAGGGGAATCACTCATCTGGCTCCAATCGCCTCTATGATCTCTACTGGCAGGCCATGAGGATGCTAG
  GAGTCCAACGTCCAAAGTCAGAAAAGAAGAATGCCAAGGATATTCCTAGTGACACCCAGAGCCCCGTCAG
  CACAAAGCGGAAGAAAAAGGGATTCTTGCCAGAGACCAAGAAGCGAAAGAAACTTAAATCTGAGGGCACC
  ACACCAGAAAAGAATGCTGCGTCACAGCAGGATGCAGTGACAGAGGGTGCCATGCCTGCTGCCACTGGTA
  AAGACCAGCCCCCCAGCACAGGCAAGAAGAAAAGGAAGAGGGTAAAGGCCAGCACCCCATCCCAGGTGAA
  TGGGATAACTGGGGCCAAGAGTCCAGCTCCCAGTAACCCCACCCTAAGCCCCAGCACCCCTGCCAAGACC
  CCAAAACTGCAGAAGAAAAAAGAGAAGCTGTCACAGGTGAATGGAGCCACTCCTGTGTCCCCCATAGAGC
  CTGAAAGCAAAAAGCATCATCAGGAGGCACTCAGCACAAAGGAGGTCATAAGAAAGTCCCCCCACCCCCA
  GTCTGCCCTGCCAAAGAAAAGAGCAAGGCTGTCTCTGGTGAGCAGGAGCCCCAGCCTGTTACAGAGTGGG
  GTCAAGAAAAGGAGAGTGGCCAGCAGGAGAGTGCAGACACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211937 representing NM_016776
  Red=Cloning site Green=Tags(s)
  
  MAEMKSPTKAEPATPAEAAQSDRHSLLEHSREFLDFFWDIAKPDQETRLRATEKLLEYLRTRPNDSEMKY
  ALKRLITGLGVGREAARPCYSLALAQLLQSFEDIPLCDILDQIQEKYSLQAMNKAMMRPSLFANLFGVLA
  LFQSGRLVKDKEALMKSVQLLKILSQHPNHLQGQPIKALVDILSEVPESMFQEILPKVLKGNMKVILRSP
  KYLELFLLAKQRVPTKLESLMGSVDLFSEDNIPSLVNILKVAANSVKKEHKLPNVALDLLRLALKESRFE
  LFWKKVLEEGLLKNPSWTSSYMCFRLLGASLPLLSEEQLQLVMRGDLIRHFGENMVISKPQNLFKIIPEI
  STYVGTFLEGCQDDPKRQLTMMVAFTTITNQGLPVMPTFWRVTRFLNAEALQSYVAWLRDMFLQPDLNSL
  VDFSTANQKRAQDASLNVPERAVFRLRKWIIHRLVSLVDHLHLEKDEAVVEQIARFCLFHAFFKTKKATP
  QIPETKQHFSFPLDDRNRGVFVSAFFSLLQTLSVKFRQTPDLAENGKPWTYRLVQLADMLLNHNRNVTSV
  TSLTTQQRQAWDQMMSTLKELEARSSETRAIAFQHLLLLVGLHIFKSPAESCDVLGDIQTCIKKSMEQNP
  RRSRSRAKASQEPVWVEVMVEILLSLLAQPSNLMRQVVRSVFGHICPHLTPRCLQLILAVLSPVTNEDED
  DNVVVTDDADEKQLQHGEDEDSDNEDNKNSESDMDSEDGEESEEEDRDKDVDPGFRQQLMEVLKAGNALG
  GVDNEEEEELGDEAMMALDQNLASLFKEQKMRIQARNEEKNKLQKEKKLRRDFQIRALDLIEVLVTKQPE
  HPLILELLEPLLNVIQHSMRSKGSTKQEQDLLHKTARIFMHHLCRARRYCHEVGPCAEALHAQVERLVQQ
  AGSQADASVALYYFNASLYLLRVLKGNTNKRHQDGHKLHGADTEDSEDQAANCLDLDFVTRVYSASLESL
  LTKRNSSLTVPMFLSLFSRYPVICKNLLPVLAQHVAGPSRPRHQAQACLMLQKTLSARELRVCFEDPEWE
  QLITQLLGKATQTLQTLGEAQSKGEHQKELSILELLNTLLRTVNHEKLSVDLTAPLGVLQSKQQKLQQSL
  QQGNHSSGSNRLYDLYWQAMRMLGVQRPKSEKKNAKDIPSDTQSPVSTKRKKKGFLPETKKRKKLKSEGT
  TPEKNAASQQDAVTEGAMPAATGKDQPPSTGKKKRKRVKASTPSQVNGITGAKSPAPSNPTLSPSTPAKT
  PKLQKKKEKLSQVNGATPVSPIEPESKKHHQEALSTKEVIRKSPHPQSALPKKRARLSLVSRSPSLLQSG
  VKKRRVASRRVQTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016776
• ORF Size: 4032 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016776.3
• RefSeq Size: 4215 bp
• RefSeq ORF: 4035 bp
• Locus ID: 18432
• UniProt ID: Q7TPV4
• Cytogenetics: 11 44.29 cM
• MW: 152 kDa
• Gene Summary: May activate or repress transcription via interactions with sequence specific DNA-binding proteins (PubMed:9447996, PubMed:11956195, PubMed:14744933). Repression may be mediated at least in part by histone deacetylase activity (HDAC activity) (PubMed:14744933). Acts as a corepressor and in concert with CRY1, represses the transcription of the core circadian clock component PER2 (PubMed:19129230). Preferentially binds to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter (PubMed:19129230). Has a role in rRNA biogenesis together with PWP1 (By similarity).[UniProtKB/Swiss-Prot Function]