Aebp1 (NM_009636) Mouse Tagged ORF Clone

CAT#: MR211674

Aebp1 (Myc-DDK-tagged) - Mouse AE binding protein 1 (Aebp1)

Product Images

Circular map for MR211674

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aebp1
• Synonyms: ACLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211674 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCAGTGCGCACCGCATCCCTGCTCTGCGGCCTCCTGGCACTGCTGACGCTGTGCCCTGAGGGGA
  ACCCACAGACGGTGCTGACGGACGACGAGATCGAGGAGTTCCTCGAAGGCTTCCTTTCGGAGTTGGAGAC
  CCAGTCCCCGCCCCGGGAAGACGACGTGGAAGTCCAGCCGCTTCCCGAACCCACCCAGCGTCCCCGCAAA
  TCCAAGGCAGGGGGCAAGCAGCGGGCAGATGTAGAAGTCCCTCCAGAAAAAAACAAAGACAAAGAGAAGA
  AAGGAAAGAAGGACAAAGGCCCCAAAGCCACAAAACCCCTGGAGGGCTCTACCAGGCCCACCAAGAAACC
  AAAGGAGAAGCCACCCAAGGCCACCAAGAAGCCCAAGGAGAAACCACCCAAGGCCACCAAGAAGCCCAAG
  GAGAAGCCACCCAAGGCCACCAAGAAGCCTAAGGAGAAGCCACCCAAGGCCACTAAGAGGCCCTCGGCAG
  GAAAGAAGTTCTCAACTGTGGCCCCCTTGGAAACGCTGGATCGGTTACTCCCCTCACCCTCCAACCCCAG
  CGCCCAGGAGCTACCGCAGAAGAGAGACACACCCTTCCCAAATGCCTGGCAAGGTCAAGGAGAAGAGACC
  CAGGTGGAGGCCAAGCAGCCCCGGCCAGAGCCAGAGGAGGAGACTGAGATGCCCACACTGGACTACAATG
  ACCAGATAGAGAAGGAGGATTACGAGGATTTTGAGTACATCCGTCGCCAGAAGCAGCCCAGGCCAACACC
  CAGCAGGAGGAGGCTCTGGCCAGAGCGCCCTGAGGAGAAGACTGAAGAGCCAGAGGAAAGGAAGGAAGTC
  GAGCCACCTCTGAAGCCCCTGCTGCCTCCGGACTATGGGGATAGCTACGTGATCCCCAACTATGATGACT
  TGGACTATTATTTCCCCCACCCTCCACCGCAGAAGCCTGATGTTGGACAAGAGGTGGATGAGGAAAAGGA
  AGAGATGAAGAAGCCCAAAAAGGAGGGTAGTAGCCCCAAGGAGGACACAGAGGACAAGTGGACCGTGGAG
  AAAAACAAGGACCACAAAGGGCCCCGGAAGGGTGAGGAGCTGGAGGAGGAGTGGGCGCCAGTGGAGAAAA
  TCAAGTGCCCACCTATTGGGATGGAGTCACACCGCATTGAGGACAACCAGATCCGTGCCTCCTCCATGCT
  GCGCCACGGCCTCGGAGCCCAGCGGGGCCGGCTCAACATGCAGGCTGGTGCCAATGAAGATGACTACTAT
  GACGGGGCATGGTGTGCTGAGGACGAGTCGCAGACCCAGTGGATCGAGGTGGACACCCGAAGGACAACTC
  GGTTCACGGGCGTCATCACTCAGGGCCGTGACTCCAGCATCCATGACGACTTCGTGACTACCTTCTTTGT
  GGGCTTCAGCAATGACAGCCAGACCTGGGTGATGTACACCAATGGCTACGAGGAAATGACCTTCTATGGA
  AATGTGGACAAGGACACACCTGTGCTGAGCGAGCTCCCTGAGCCAGTTGTGGCCCGTTTCATCCGCATCT
  ATCCACTCACCTGGAACGGTAGCCTGTGCATGCGCCTGGAGGTGCTAGGCTGCCCCGTGACCCCTGTCTA
  CAGCTACTACGCACAGAATGAGGTGGTAACTACTGACAGCCTGGACTTCCGGCACCACAGCTACAAGGAC
  ATGCGCCAGCTGATGAAGGCTGTCAATGAGGAGTGCCCCACAATCACTCGCACATACAGCCTGGGCAAGA
  GTTCACGAGGGCTCAAGATCTACGCAATGGAAATCTCAGACAACCCTGGGGATCATGAACTGGGGGAGCC
  CGAGTTCCGCTACACAGCCGGGATCCACGGCAATGAGGTGCTAGGCCGAGAGCTCCTGCTCCTGCTCATG
  CAATACCTATGCCAGGAGTACCGCGATGGGAACCCGAGAGTGCGCAACCTGGTGCAGGACACACGCATCC
  ACCTGGTGCCCTCGCTGAACCCTGATGGCTATGAGGTGGCAGCGCAGATGGGCTCAGAGTTTGGGAACTG
  GGCACTGGGGCTGTGGACTGAGGAGGGCTTTGACATCTTCGAGGACTTCCCAGATCTCAACTCTGTGCTC
  TGGGCAGCTGAGGAGAAGAAATGGGTCCCCTACAGGGTCCCAAACAATAACTTGCCAATCCCTGAACGTT
  ACCTGTCCCCAGATGCCACGGTCTCCACAGAAGTCCGGGCCATTATTTCCTGGATGGAGAAGAACCCCTT
  TGTGCTGGGTGCAAATCTGAACGGTGGTGAGCGGCTTGTGTCTTATCCCTATGACATGGCCCGGACACCT
  AGCCAGGAGCAGCTGTTGGCCGAGGCACTGGCAGCTGCCCGCGGAGAAGATGATGACGGGGTGTCTGAGG
  CCCAGGAGACTCCAGATCACGCTATTTTCCGCTGGCTGGCCATCTCATTTGCCTCCGCCCATCTCACCAT
  GACGGAGCCCTACCGGGGAGGGTGCCAGGCCCAGGACTACACCAGCGGCATGGGCATTGTCAACGGGGCC
  AAGTGGAATCCTCGCTCTGGGACTTTCAATGACTTTAGCTACCTGCACACAAACTGTCTGGAGCTCTCCG
  TATACCTGGGCTGTGACAAGTTCCCCCACGAGAGTGAGCTACCCCGAGAATGGGAGAACAACAAAGAAGC
  GCTGCTCACCTTCATGGAGCAGGTGCACCGTGGCATTAAGGGTGTGGTGACAGATGAGCAAGGCATCCCC
  ATTGCCAATGCCACCATCTCTGTGAGTGGCATCAACCATGGTGTGAAGACAGCAAGTGGAGGTGACTACT
  GGCGCATTCTGAACCCGGGTGAGTACCGTGTGACAGCTCACGCAGAGGGCTACACCTCAAGTGCCAAGAT
  CTGCAATGTGGACTACGATATTGGGGCCACTCAGTGCAACTTCATCCTGGCTCGATCCAACTGGAAGCGC
  ATTCGGGAGATCTTGGCTATGAACGGGAACCGTCCCATTCTCCGAGTTGACCCCTCACGACCCATGACCC
  CCCAGCAGCGGCGCATGCAGCAGCGCCGTCTACAGTACCGGCTCCGCATGAGGGAACAGATGCGACTGCG
  TCGCCTCAATTCTACCGCAGGCCCTGCCACAAGCCCCACTCCTGCCCTTATGCCTCCCCCTTCCCCTACA
  CCAGCCATTACCTTGAGGCCCTGGGAAGTTCTACCCACTACCACTGCAGGCTGGGAGGAGTCAGAGACTG
  AGACCTATACAGAAGTAGTGACAGAGTTTGAGACAGAGTATGGGACTGACCTAGAGGTGGAAGAGATAGA
  GGAGGAGGAGGAGGAGGAGGAGGAAGAGATGGACACAGGCCTTACATTTCCACTCACAACAGTGGAGACC
  TACACAGTGAACTTTGGGGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211674 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAPVRTASLLCGLLALLTLCPEGNPQTVLTDDEIEEFLEGFLSELETQSPPREDDVEVQPLPEPTQRPRK
  SKAGGKQRADVEVPPEKNKDKEKKGKKDKGPKATKPLEGSTRPTKKPKEKPPKATKKPKEKPPKATKKPK
  EKPPKATKKPKEKPPKATKRPSAGKKFSTVAPLETLDRLLPSPSNPSAQELPQKRDTPFPNAWQGQGEET
  QVEAKQPRPEPEEETEMPTLDYNDQIEKEDYEDFEYIRRQKQPRPTPSRRRLWPERPEEKTEEPEERKEV
  EPPLKPLLPPDYGDSYVIPNYDDLDYYFPHPPPQKPDVGQEVDEEKEEMKKPKKEGSSPKEDTEDKWTVE
  KNKDHKGPRKGEELEEEWAPVEKIKCPPIGMESHRIEDNQIRASSMLRHGLGAQRGRLNMQAGANEDDYY
  DGAWCAEDESQTQWIEVDTRRTTRFTGVITQGRDSSIHDDFVTTFFVGFSNDSQTWVMYTNGYEEMTFYG
  NVDKDTPVLSELPEPVVARFIRIYPLTWNGSLCMRLEVLGCPVTPVYSYYAQNEVVTTDSLDFRHHSYKD
  MRQLMKAVNEECPTITRTYSLGKSSRGLKIYAMEISDNPGDHELGEPEFRYTAGIHGNEVLGRELLLLLM
  QYLCQEYRDGNPRVRNLVQDTRIHLVPSLNPDGYEVAAQMGSEFGNWALGLWTEEGFDIFEDFPDLNSVL
  WAAEEKKWVPYRVPNNNLPIPERYLSPDATVSTEVRAIISWMEKNPFVLGANLNGGERLVSYPYDMARTP
  SQEQLLAEALAAARGEDDDGVSEAQETPDHAIFRWLAISFASAHLTMTEPYRGGCQAQDYTSGMGIVNGA
  KWNPRSGTFNDFSYLHTNCLELSVYLGCDKFPHESELPREWENNKEALLTFMEQVHRGIKGVVTDEQGIP
  IANATISVSGINHGVKTASGGDYWRILNPGEYRVTAHAEGYTSSAKICNVDYDIGATQCNFILARSNWKR
  IREILAMNGNRPILRVDPSRPMTPQQRRMQQRRLQYRLRMREQMRLRRLNSTAGPATSPTPALMPPPSPT
  PAITLRPWEVLPTTTAGWEESETETYTEVVTEFETEYGTDLEVEEIEEEEEEEEEEMDTGLTFPLTTVET
  YTVNFGDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009636
• ORF Size: 3387 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009636.3
• RefSeq Size: 4049 bp
• RefSeq ORF: 3387 bp
• Locus ID: 11568
• UniProt ID: Q640N1
• Cytogenetics: 11 A1
• MW: 128.4 kDa
• Gene Summary: Isoform 1: As a positive regulator of collagen fibrillogenesis, it is probably involved in the organization and remodeling of the extracellualr matrix.[UniProtKB/Swiss-Prot Function]