Arhgef10 (BC059212) Mouse Tagged ORF Clone

CAT#: MR211634

Arhgef10 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 10 (cDNA clone MGC:66545 IMAGE:6400425)

Product Images

Circular map for MR211634

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arhgef10
• Synonyms: 6430549H08Rik; mKIAA0294
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211634 representing BC059212
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAATCCAGAGGAAGCAATTTACGACGACGTTCCGAGGGAAAACTCGGACTCTGAGCCAGATGAAA
  TGATTTATGATGATGTGGAGAACGGCGAAGAAGGTGGAAACAGCTCCCCAGAGTACGGGTGGAGTTCCAG
  CGAATTCGAAAGCTATGAGGAGCCAAGCGACTCGGAGGGCAAGAACGGAATTCCCAGGTCCTTCCTGCGC
  AGCAGTCATAAAAAGCAAATGCAGAAGCTTATGAAGGCAGCCAAGGAGGGCACCAAAGATGGGCTGGAGA
  AAACCAAAGCTGCTGTGAAGAGAGGCGGCTCCTTCATCAGGACCAGGTCCTTGGTCTCTCAAGATCACAG
  GTGTTATTTTGAAGAAGAGCAGAATTTGTTCATTGACGTGGACTGCAAGCACCCGGAGGCTGTCCTCACC
  CCAATGCCCGAGGGCTTGTCTCAGCAGCAGGTTGTGAGAAGATACATTCTGGGCTCCATTGTTGAGAGCG
  AGAAGAACTATGTAGATGCTCTCCGGAGGATTTTGGAGCAATATGAGAAGCCGCTGTCTGAGATGGAGCC
  CCGGCTCCTGAGTGACAGGAAGCTCAGGATGGTTTTCTACCGCGTCAAGGAGATCCTGCAGTGTCACTCC
  ATGTTCCAGATTGCGCTGGCCAGCCGCGTGTCCGAGTGGGATGTCGTGGAAACAATCGGCGACGTTTTTG
  TGGCCTCGTTCTCCAAGTCCATGGTGCTGGATGCTTACAGCGAGTACGTGAACAACTTCAGCACGGCCGT
  GGCTGTCCTCAAGAAGACGTGTGCCACAAAGCCTGCTTTCCTTGAGTTCTTAAAGCTGAGCCAGGATTCC
  AGTCCAGACCGAGTCACACTCCACAGCCTTATGATGCGGCCCATCCAGAGGTTCCCACAGTTCATCCTCC
  TACTGCAGGACATGCTGAAGAACACCGCCAAAGGACATCCTGACAGGCTGCCCCTTCAGATGGCGCTGAC
  GGAGCTCGAGACCCTGGCAGAGAAGCTAAACGAGAGAAAAAGAGATGCCGACCAGCGATGCGAAATCAAA
  CAAATAGCAAAAGCCATAAACGAGAGATACCTGAACAAGCTCCTCAGCAGTGGAAACCGGTACCTCGTCC
  GGTCCGATGACGTCATCGAGACGGTTTACAACGACCGGGGAGAAATCGTCAAGACCAAACAACGCCGGAT
  CTTCATGCTGAATGACGTGCTCATGTGTGCCACCGCCAGCTCACGGAACTCACATGAAAGCCATGCCGTG
  ATGAGCCAGAGGTACCTGCTCAAGTGGAGTGTCCCTCTAGGTCACGTGGATGTGATTCAGTACAATGGAG
  GCTCTGGCGCCGGGGAGCACTGTCGGCACCACGCTGCACACTCACCTGAGAGCCTGGCCGTGGTGGCCAA
  CGCGAAGCCACACAAAGTTTACATGGGGCCAGGGCAGCTGTACCAGGATCTGCAGAACCTACTGCACGAC
  CTGAACGTTGTTGGCCAGATCTCGCAGCTGATAGGAAACCTCCGAGGGAGCTATCAGAACTTAAACCAGT
  CTGTAGCCCATGATTGGACGTCGGGCTTACAGCGGCTGATACTGAGGAAAGAAGATGCGATCAGAGCTGC
  CGACCGCTGCCGGATCCAGTTACAGCTCCCGGGAAAGCAGGACAAGTCCGGGCGGCCCACGTTCTTCACT
  GCTGTGTTCAATACGCTTACACCAGCCATCAAGGAGTCCTGGGTCAGCAGCCTGCAGATGGCCAAGCTGG
  CCCTTGAGGAGGAGAACCATATGGGATGGTTCTGTGTGGATGACGATGGCAACCTAGCCAAGAAGGAGAC
  ACACCCTCTCCTGGTGGGACACATGCCTGTGATGGTGGCCAAGCAGCCAGAGTTCAAGATTGAATGTGCA
  GCTTATAACCCTGAACCTTACCTGAGCAATGAAAGTCAACCAGCCTCACCCTCCACAGCCCGTGGCTTTC
  TGTGGATTGGAAGCTGTAGCAACCAGATGGGTCAGGTCGCCATTGTCTCCTTCCAAGGTTCCAACCCCAA
  AGTCATCGAGTGCTTCAATGTGGAATCACGCATCCTGTGTATGGTATACATTCCCGCAGAGGAGTCAAAG
  CCCCAAGAAACCACCGAAACCAAGGATCCAGAAGCCACAGCCTCGAGAGCACCCCATGTGCCCACCATCT
  GCCTGGGCACAGAAGAGGGAAGCATTTCCATTTACAAAAGCAGTCAGGGCTGCAAGAAGGTGAGGCTTCA
  GCACTTCTATGCACCGGACAAGTCCACGGTCATGAGCTTGGCCTGCTCCCCACAAGGCCTGTATGCAGGC
  CTGGTCAATGGGTCCGTAGCCAGCTACACCAAGGCTCCAGATGGATCCTGGAACTCGGAACCCCAGCAAG
  TGATCAAGTTGGGTGTCCTGCCAGTCAGAAGCCTTCTCCTGGTGGAGGGTGCACTGTGGGCAGCCTCGGG
  AGGGCAGGTGTTCATGGCCAGCGTGGAGACTCACACTATAGAGAATCAGCTGGAGGCCCACCAAGATGAA
  GGGATGGTCATCTCCCACATGGCTGTGGCTGGCGTTGGCATCTGGATTGCCTTCACCTCGGGGTCCACCC
  TCCGCCTCTTCCACACAGAGACCTTGAAGCACCTGCAGGATGTCAACATTGATGCCCCTGTGCATAGCAT
  GCTGCCAGGGCACCAGCGTCTATCTGTGACCAGCCTGCTCGTCTGCCACGGCTTGCTCATGGTCGGTACC
  AGCCTGGGTGTCGTGGTGGCCCTGCCAGTCCCTCGGCTTCAGGGTATCCCCAAAGTGACAGGGCGAGGCA
  TGGTCTCCTACCATGCGCATAACGGGCCCGTTAAGTTCATCGTCTCAGCCACGGCGTTTCAAAACAAGGA
  CAGAGCCAGGGACAGCCCACGTTCCGGTTCTGAGCTTCAGGATGAAGACCCCAAGGACTTGCTCTGCAGC
  GAGGAAGGTCCTTCCTGCCCGGGCCAGCCTGACACCTACACATCCGTTTGGTTGGGAGACTCCCTAGGAT
  TGCCAACTCAAAAGAATGATCTGTCTTCGTCCTCGGGGTCACTGAACCTGTCCCATGGCTCCAGTTCCCT
  AGAGCACAGATCTGTGGACAGCAGTCTGTGTGACCTGCTGAGGGACCCCAGTGCCTCCCCAAGGAGCAGA
  CCACAGGGCTCTAGGCGAGCAAGAGCCAGTTCAGCGCTGGTGGTATGTGGGGGCCAGGGCCACCGGCGGG
  TGCACAGGAAGGCCAGGCAGCCTTCTCAGGAGGACCTGGTTTCTTCAGTGATGGTGTGGCAGATCCCCCT
  GCTGGGCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211634 representing BC059212
  Red=Cloning site Green=Tags(s)
  
  MENPEEAIYDDVPRENSDSEPDEMIYDDVENGEEGGNSSPEYGWSSSEFESYEEPSDSEGKNGIPRSFLR
  SSHKKQMQKLMKAAKEGTKDGLEKTKAAVKRGGSFIRTRSLVSQDHRCYFEEEQNLFIDVDCKHPEAVLT
  PMPEGLSQQQVVRRYILGSIVESEKNYVDALRRILEQYEKPLSEMEPRLLSDRKLRMVFYRVKEILQCHS
  MFQIALASRVSEWDVVETIGDVFVASFSKSMVLDAYSEYVNNFSTAVAVLKKTCATKPAFLEFLKLSQDS
  SPDRVTLHSLMMRPIQRFPQFILLLQDMLKNTAKGHPDRLPLQMALTELETLAEKLNERKRDADQRCEIK
  QIAKAINERYLNKLLSSGNRYLVRSDDVIETVYNDRGEIVKTKQRRIFMLNDVLMCATASSRNSHESHAV
  MSQRYLLKWSVPLGHVDVIQYNGGSGAGEHCRHHAAHSPESLAVVANAKPHKVYMGPGQLYQDLQNLLHD
  LNVVGQISQLIGNLRGSYQNLNQSVAHDWTSGLQRLILRKEDAIRAADRCRIQLQLPGKQDKSGRPTFFT
  AVFNTLTPAIKESWVSSLQMAKLALEEENHMGWFCVDDDGNLAKKETHPLLVGHMPVMVAKQPEFKIECA
  AYNPEPYLSNESQPASPSTARGFLWIGSCSNQMGQVAIVSFQGSNPKVIECFNVESRILCMVYIPAEESK
  PQETTETKDPEATASRAPHVPTICLGTEEGSISIYKSSQGCKKVRLQHFYAPDKSTVMSLACSPQGLYAG
  LVNGSVASYTKAPDGSWNSEPQQVIKLGVLPVRSLLLVEGALWAASGGQVFMASVETHTIENQLEAHQDE
  GMVISHMAVAGVGIWIAFTSGSTLRLFHTETLKHLQDVNIDAPVHSMLPGHQRLSVTSLLVCHGLLMVGT
  SLGVVVALPVPRLQGIPKVTGRGMVSYHAHNGPVKFIVSATAFQNKDRARDSPRSGSELQDEDPKDLLCS
  EEGPSCPGQPDTYTSVWLGDSLGLPTQKNDLSSSSGSLNLSHGSSSLEHRSVDSSLCDLLRDPSASPRSR
  PQGSRRARASSALVVCGGQGHRRVHRKARQPSQEDLVSSVMVWQIPLLGM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC059212
• ORF Size: 3300 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC059212.1
• RefSeq Size: 4065 bp
• RefSeq ORF: 3302 bp
• Locus ID: 234094
• Cytogenetics: 8 A1.1
• MW: 149.1 kDa
• Gene Summary: May play a role in developmental myelination of peripheral nerves.[UniProtKB/Swiss-Prot Function]