Itga3 (NM_013565) Mouse Tagged ORF Clone

CAT#: MR211548

Itga3 (Myc-DDK-tagged) - Mouse integrin alpha 3 (Itga3)

Product Images

Circular map for MR211548

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itga3
• Synonyms: AA407068; CD49C; GAPB3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211548 representing NM_013565
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCCGGCCCCTGCCGCGTGCCCCGCGCCCCTGGCTGGCTGCTCCGCGCTCTGGCCTTGATGGTGG
  CGGCTTGCGGCCGGGTCGCCTTCGCCTTCAACCTGGACACCCGATTCCTGGTGGTGAAGGAGGCGGTGAA
  CCCCGGTAGCCTCTTCGGCTACTCGGTCGCCCTCCATCGGCAGACTGAGCGACAACAGCGCTACCTCCTT
  CTGGCCGGGGCTCCCCGGGACCTCGCTGTGGGTGATGACTATACCAACCGGACTGGTGCTGTGTACCTGT
  GTCCCCTCACGGCCCACAAGGACGACTGTGAACGGATGGACATTTCAGAGAAAAGTGACCCTGACCATCA
  CATTATTGAGGATATGTGGCTTGGAGTGACTGTGGCCAGCCAGGGCCCTGCAGGTAGAGTCCTGGTCTGT
  GCCCATCGGTACACCAAGGTGCTGTGGTCTGGGCTAGAAGACCAGCGGCGCATGGTGGGCAAGTGCTATG
  TGCGTGGCAATGACCTACAGCTCGACCCCGGCGATGACTGGCAGACATACCACAACGAGATGTGTAACAG
  CAACACTGACTACTTGCAGACCGGCATGTGCCAGCTGGGCACCAGCGGCGGCTTCACCCAGAACACCGTA
  TACTTCGGTGCCCCTGGTGCCTACAACTGGAAAGGAAACAGCTACATGATTCAGCGGAAGGACTGGGATT
  TATCTGAATATAGCTACAGGGGCTCAGAGGAGCAAGGAAACCTTTATATTGGGTACACGGTGCAGGTAGG
  CAACGCCATCCTACATCCCACGGACATCATCACTGTTGTGACGGGTGCCCCACGGCACCAACATATGGGC
  GCTGTCTTCTTGCTGAAGCAAGAGTCAGGTGGAGACCTGCAGAGGAAGCAGGTGCTGAAGGGCACGCAGG
  TGGGCGCTTATTTTGGCAGTGCCATTGCCCTGGCAGACCTGAACAATGATGGGTGGCAGGACCTCCTGGT
  GGGTGCTCCCTACTACTTCGAACGGAAAGAGGAGGTAGGGGGTGCCGTCTATGTCTTCATGAACCAGGCG
  GGCGCATCCTTCCCTGATCAACCTTCCCTCCTGCTTCACGGCCCCAGCCGCTCTGCCTTTGGCATCTCTA
  TAGCCAGCATCGGTGACATCAACCAGGATGGATTCCAGGACATCGCTGTGGGAGCCCCATTTGAGGGCTT
  GGGCAAAGTCTACATCTACCACAGCAGCTCCGGGGGGCTCCTCAGGCAGCCCCAGCAGATAATCCATGGA
  GAGAAACTCGGACTGCCAGGCTTGGCCACCTTCGGCTACTCCCTGAGCGGGAAGATGGATGTGGATGAAA
  ACCTTTACCCAGACCTGCTAGTAGGGAGCCTGTCGGACCACATCGTGCTGCTGCGGGCCCGGCCTGTCAT
  CAATATCCTCCATAGGACCTTGGTGGCCAGGCCAGCTGTGTTGGACCCTGCGCTTTGTACAGCTACCTCC
  TGTGTTCAGGTGGAGCTGTGTTTTGCCTACAACCAGAGCGCTGGGAACCCCAACTACAGGCGGAACATCA
  CCCTGGCTTACACACTGGAGGCTGACAGGGACCGACGCCCACCCAGGCTCCGATTTGCCCGCAGCCAGTC
  ATCTGTCTTCCACGGCTTCTTCTCCATGCCAGAGACACATTGCCAGACACTGGAGTTACTGCTGATGGAC
  AATGTTCGCGATAAACTCCGTCCTATCGTCATTGCCATGAACTACTCCTTACCTCTGCGCATGCCTGATC
  GCCTCAAGCTCGGCTTGCGGTCTCTGGATGCCTACCCAGTCCTCAACCAGGCACAGGCTATGGAGAATCA
  CACTGAGGTCCACTTCCAGAAAGAGTGCGGGCCAGACAACAAGTGCGACAGCAACCTGCAGATGCGAGCA
  GCCTTCCTGTCTGAGCAGCTGCAGCCTCTAAGCAGGCTCCAGTACAGCAGAGACACTAAGAAACTGTTTT
  TGAGCATCAATGTGACCAATTCACCGAGCAGCCAACGGGCTGGGGAAGATGCGCATGAGGCATTGCTCAC
  CCTAGAGGTGCCATCCGCCCTGCTACTGTCCTCCGTGCGCCCGTCTGGGACCTGCCAAGCTAATAACGAG
  ACCATCCTGTGTGAGCTAGGAAACCCCTTCAAACGGAACCAGAGGATGGAGCTGCTCATTGCCTTTGAGG
  TCATCGGGGTAACGTTGCACACGAGGGACCTCCCAGTACTTCTTCAGCTGTCCACGTCGAGTCACCAGGA
  CAACTTGCAGCCCGTACTCCTGACTTTGCAGGTGGACTACACCCTCCAGGCCTCGCTCAGCTTAATGAAT
  CATCGGTTGCAGAGCTTCTTTGGTGGCACAGTGATGGGTGAGGCTGCCATGAAAACTGCGGAGGATGTGG
  GAAGTCCCCTGAAATATGAATTCCAGGTGAGCCCAGTGGGAGATGGGCTGGCAGCCTTGGGCACACTGGT
  TCTAGGTCTGGAGTGGCCCTATGAAGTTACCAATGGCAAGTGGCTGCTGTACCCCACGGAGATCACCATC
  CATAGCAATGGTTCCTGGCCCTGCCAGCCGTCTGGAAACCTTGTCAACCCTCTCAACCTTACTCTCTCTG
  ACCCTGGAGTCACACCACTGTCGCCACAGCGCCGCCGGCGGCAACTGGATCCAGGGGGAGACCAGAGTTC
  CCCGCCTGTCACACTAGCTGCTGCCAAAAAAGCCAAGTCTGAGACTGTGTTGACCTGCTCCAATGGCCGT
  GCCCGCTGTGTGTGGCTGGAGTGCCCCCTTCCAGACACCTCCAACATTACCAATGTGACCGTGAAAGCAC
  GGGTGTGGAACAGCACCTTCATTGAGGACTACAAAGACTTTGACAGAGTCAGGGTAGATGGCTGGGCTAC
  CCTGTTCCTGAGAACCAGCATCCCTACCATCAACATGGAGAACAAGACCACATGGTTCTCTGTGGACATT
  GACTCAGAGCTGGTGGAGGAGCTGCCGGCTGAGATTGAGCTGTGGTTGGTGCTTGTGGCCGTGGGTGCTG
  GGTTGCTGCTGCTGGGGCTCATCATCCTCCTCTTGTGGAAGTGCGGCTTCTTCAAGCGAGCCCGCACTCG
  TGCCCTGTATGAAGCTAAGAGGCAGAAGGCTGAGATGAAGAGCCAGCCGTCAGAGACAGAAAGGCTGACC
  GACGACTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211548 representing NM_013565
  Red=Cloning site Green=Tags(s)
  
  MGPGPCRVPRAPGWLLRALALMVAACGRVAFAFNLDTRFLVVKEAVNPGSLFGYSVALHRQTERQQRYLL
  LAGAPRDLAVGDDYTNRTGAVYLCPLTAHKDDCERMDISEKSDPDHHIIEDMWLGVTVASQGPAGRVLVC
  AHRYTKVLWSGLEDQRRMVGKCYVRGNDLQLDPGDDWQTYHNEMCNSNTDYLQTGMCQLGTSGGFTQNTV
  YFGAPGAYNWKGNSYMIQRKDWDLSEYSYRGSEEQGNLYIGYTVQVGNAILHPTDIITVVTGAPRHQHMG
  AVFLLKQESGGDLQRKQVLKGTQVGAYFGSAIALADLNNDGWQDLLVGAPYYFERKEEVGGAVYVFMNQA
  GASFPDQPSLLLHGPSRSAFGISIASIGDINQDGFQDIAVGAPFEGLGKVYIYHSSSGGLLRQPQQIIHG
  EKLGLPGLATFGYSLSGKMDVDENLYPDLLVGSLSDHIVLLRARPVINILHRTLVARPAVLDPALCTATS
  CVQVELCFAYNQSAGNPNYRRNITLAYTLEADRDRRPPRLRFARSQSSVFHGFFSMPETHCQTLELLLMD
  NVRDKLRPIVIAMNYSLPLRMPDRLKLGLRSLDAYPVLNQAQAMENHTEVHFQKECGPDNKCDSNLQMRA
  AFLSEQLQPLSRLQYSRDTKKLFLSINVTNSPSSQRAGEDAHEALLTLEVPSALLLSSVRPSGTCQANNE
  TILCELGNPFKRNQRMELLIAFEVIGVTLHTRDLPVLLQLSTSSHQDNLQPVLLTLQVDYTLQASLSLMN
  HRLQSFFGGTVMGEAAMKTAEDVGSPLKYEFQVSPVGDGLAALGTLVLGLEWPYEVTNGKWLLYPTEITI
  HSNGSWPCQPSGNLVNPLNLTLSDPGVTPLSPQRRRRQLDPGGDQSSPPVTLAAAKKAKSETVLTCSNGR
  ARCVWLECPLPDTSNITNVTVKARVWNSTFIEDYKDFDRVRVDGWATLFLRTSIPTINMENKTTWFSVDI
  DSELVEELPAEIELWLVLVAVGAGLLLLGLIILLLWKCGFFKRARTRALYEAKRQKAEMKSQPSETERLT
  DDY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013565
• ORF Size: 3159 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013565.3, NP_038593.1
• RefSeq Size: 4870 bp
• RefSeq ORF: 3162 bp
• Locus ID: 16400
• UniProt ID: Q62470
• Cytogenetics: 11 59.01 cM
• MW: 117.2 kDa
• Gene Summary: This gene encodes a subunit of integrin family of cell surface proteins. The encoded protein undergoes post-translational processing to form a disulfide bond-linked dimer comprised of heavy and light chains. At the cell surface, the encoded protein non-covalently associates with the integrin beta-1 subunit to form a heterodimer that interacts with many extracellular matrix proteins including fibronectin and laminin. Mice lacking the encoded protein die during the first day after birth due to severe abnormalities in kidneys. Mice lacking the encoded protein specifically in the basal layer of epidermis display several skin defects and accelerated wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]