Itga5 (NM_010577) Mouse Tagged ORF Clone

CAT#: MR211547

Itga5 (Myc-DDK-tagged) - Mouse integrin alpha 5 (fibronectin receptor alpha) (Itga5)

Product Images

Circular map for MR211547

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itga5
• Synonyms: Cd49e; F; Fnra; VLA5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211547 representing NM_010577
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGCTGGACGCCACGGTCGCCTCGATCTCCTCTCCACGCGGTGCTGCTGCGCTGGGGGCCCCGAC
  GCCTACCGCCGCTGCTGCCTCTGCTGCTGCTACTGTGGCCGCCACCACTCCAGGTTGGGGGCTTCAACCT
  AGACGCGGAGGCCCCGGCGGTGCTCTCCGGGCCCCCCGGCTCCCTTTTTGGCTTCTCCGTGGAGTTTTAC
  CGGCCGGGAAGGGACGGAGTCAGTGTGCTGGTTGGGGCACCCAAGGCTAACACTAGCCAGCCAGGTGTAC
  TGCAAGGTGGTGCTGTCTATGTGTGTCCCTGGGGCACCAGTCCTATCCAGTGCACCACCATTCAATTTGA
  CAGCAAAGGCTCCCGGATTCTGGAGTCCTCACTGTACAGTGCCAAGGGAGAGGAGCCTGTGGAGTACAAG
  TCCTTGCAGTGGTTCGGAGCAACAGTTCGGGCCCATGGCTCCTCCATCTTGGCATGTGCTCCACTGTATA
  GCTGGCGCACAGAAAAGGACCCACAGAATGACCCAGTGGGCACCTGCTACCTCTCCACAGAAAACTTCAC
  CCGGATTCTGGAGTACGCACCTTGCCGCTCAGATTTTGGCAGTGCAGCAGGGCAGGGCTACTGCCAAGGG
  GGCTTCAGTGCTGAGTTCACCAAGACTGGCCGTGTGGTCCTGGGTGGACCTGGAAGCTACTTCTGGCAAG
  GCCAGATCCTGTCCGCCACTCAAGAGCAGATCTCGGAGTCCTATTACCCAGAGTATCTCATCAACCCTGT
  TCAGGGGCAGCTGCAGACCCGCCAGGCCAGCTCCGTCTATGATGACAGCTACTTGGGATACTCTGTGGCT
  GTGGGTGAATTCAGTGGTGATGACACAGAAGACTTTGTTGCTGGCGTGCCCAAGGGGAACCTCACCTATG
  GCTATGTCACCGTCCTTAATGGCTCAGACATCCACTCCCTCTACAACGTCTCAGGAGAACAGATGGCCTC
  CTACTTCGGCTATGCTGTGGCTGCCACTGATACCAATGGAGATGGGCTAGATGACCTACTGGTAGGGGCA
  CCCCTGCTCATGGAGCGGACAGCTGATGGGAGACCTCAGGAGGTGGGCAGGGTCTACATCTATCTGCAGC
  GCCCAGCGGGCATAGATCCCACACCCACCCTCACCCTCACTGGGCAAGATGAGTTCAGCCGATTCGGCAG
  CTCCTTGACACCCTTGGGGGACCTGGACCAAGACGGCTACAATGATGTCGCCATTGGGGCTCCATTTGGT
  GGGGAGGCCCAGCAGGGAGTCGTATTTATATTCCCGGGAGGCCCAGGAGGACTGAGCACTAAACCTTCCC
  AGGTTTTGCAGCCTCTTTGGGCAGCTGGCCGTACCCCAGACTTCTTTGGCTCTGCCCTTCGAGGAGGACG
  AGATCTGGATGGCAATGGATACCCTGATCTAATCGTTGGATCCTTTGGTGTGGACAAGGCTCTGGTGTAC
  AGAGGGCGGCCCATCATATCTGCCAGCGCATCTCTCACCATCTTCCCCTCCATGTTCAACCCAGAGGAGC
  GCAGTTGCAGCCTGGAAGGGAACCCTGTGTCCTGCATCAACCTTAGCTTCTGCCTCAATGCCTCTGGAAA
  ACATGTCCCCAACTCTATAGGCTTCGAGGTGGAACTCCAACTGGACTGGCAGAAGCAAAAGGGAGGGGTC
  CGGCGGGCACTGTTCCTGACTTCCAAGCAGGCCACCCTTACCCAGACCCTGCTTATCCAGAATGGGGCTC
  GGGAGGACTGCAGGGAGATGAAGATCTACCTCAGGAATGAATCAGAATTCAGAGACAAACTCTCCCCAAT
  TCACATTGCCCTCAACTTCTCCTTGGACCCCAAAGCTCCCATGGACAGCCATGGCCTCCGGCCAGTTCTA
  CACTACCAAAGCAAAAGCAGGATAGAGGACAAGGCCCAGATCTTGCTGGACTGTGGTGAAGACAATATCT
  GTGTGCCTGACCTGCAGCTGGATGTGTATGGGGAGAAGAAACATGTGTACCTGGGTGACAAGAACGCACT
  GAACCTGACATTCCATGCCCAAAATCTGGGTGAGGGCGGTGCCTATGAAGCCGAGCTTCGGGTCACAGCC
  CCTCTAGAGGCCGAGTACTCAGGACTTGTCAGACACCCAGGGAACTTCTCCAGCCTGAGCTGTGACTACT
  TTGCTGTGAACCAGAGCCGCCAGCTGGTGTGTGACCTGGGCAACCCCATGAAGGCAGGCACCAGTCTCTG
  GGGTGGCCTTCGGTTCACTGTTCCTCATCTTCAAGACACAAAGAAAACCATCCAGTTTGACTTTCAGATC
  CTCAGCAAGAACCTGAACAACTCACAAAGCAACGTGGTCTCCTTCCCACTCTCGGTGGAGGCTCAAGCCC
  AGGTCTCCCTTAATGGTGTCTCCAAGCCTGAAGCTGTGATTTTCCCAGTCAGCGACTGGAATCCTCAAGA
  CCAGCCTCAGAAGGAGGAAGACTTGGGCCCAGCTGTCCACCATGTCTACGAGCTCATCAACCAGGGGCCC
  AGCTCCATCAGCCAGGGTGTGCTGGAGCTCAGCTGTCCACAGGCTCTGGAAGGCCAACAGCTCCTCTATG
  TGACCAAGGTGACAGGACTCAGCAACTGCACCTCCAACTACACCCCCAACTCACAGGGCCTGGAGTTGGA
  TCCAGAGACCTCTCCACACCACCTGCAGAAACGAGAGGCTCCAGGGAGGAGTTCTACTGCCTCAGGAACA
  CAAGTTCTGAAATGCCCTGAAGCCAAGTGTTTCAGGCTGCGCTGTGAGTTTGGGCCACTGCACCGCCAAG
  AGAGCCGTAGTCTGCAGCTGCATTTCCGAGTCTGGGCCAAGACCTTCTTGCAGCGGGAATACCAGCCATT
  TAGCCTTCAGTGTGAGGCTGTATATGAAGCTCTGAAGATGCCCTACCAGATCCTGCCTCGGCAGCTTCCC
  CAAAAGAAACTTCAGGTGGCCACAGCCGTGCAGTGGACCAAGGCAGAAGGCAGCAATGGTGTCCCGTTGT
  GGATCATCATCCTAGCCATTCTTTTTGGCCTCCTGCTCCTAGGTCTGCTCATCTACGTCCTCTACAAGCT
  CGGCTTCTTCAAACGTTCCCTCCCCTACGGCACAGCCATGGAAAAAGCTCAGCTCAAGCCTCCAGCCACC
  TCAGATGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211547 representing NM_010577
  Red=Cloning site Green=Tags(s)
  
  MGSWTPRSPRSPLHAVLLRWGPRRLPPLLPLLLLLWPPPLQVGGFNLDAEAPAVLSGPPGSLFGFSVEFY
  RPGRDGVSVLVGAPKANTSQPGVLQGGAVYVCPWGTSPIQCTTIQFDSKGSRILESSLYSAKGEEPVEYK
  SLQWFGATVRAHGSSILACAPLYSWRTEKDPQNDPVGTCYLSTENFTRILEYAPCRSDFGSAAGQGYCQG
  GFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQISESYYPEYLINPVQGQLQTRQASSVYDDSYLGYSVA
  VGEFSGDDTEDFVAGVPKGNLTYGYVTVLNGSDIHSLYNVSGEQMASYFGYAVAATDTNGDGLDDLLVGA
  PLLMERTADGRPQEVGRVYIYLQRPAGIDPTPTLTLTGQDEFSRFGSSLTPLGDLDQDGYNDVAIGAPFG
  GEAQQGVVFIFPGGPGGLSTKPSQVLQPLWAAGRTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKALVY
  RGRPIISASASLTIFPSMFNPEERSCSLEGNPVSCINLSFCLNASGKHVPNSIGFEVELQLDWQKQKGGV
  RRALFLTSKQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPKAPMDSHGLRPVL
  HYQSKSRIEDKAQILLDCGEDNICVPDLQLDVYGEKKHVYLGDKNALNLTFHAQNLGEGGAYEAELRVTA
  PLEAEYSGLVRHPGNFSSLSCDYFAVNQSRQLVCDLGNPMKAGTSLWGGLRFTVPHLQDTKKTIQFDFQI
  LSKNLNNSQSNVVSFPLSVEAQAQVSLNGVSKPEAVIFPVSDWNPQDQPQKEEDLGPAVHHVYELINQGP
  SSISQGVLELSCPQALEGQQLLYVTKVTGLSNCTSNYTPNSQGLELDPETSPHHLQKREAPGRSSTASGT
  QVLKCPEAKCFRLRCEFGPLHRQESRSLQLHFRVWAKTFLQREYQPFSLQCEAVYEALKMPYQILPRQLP
  QKKLQVATAVQWTKAEGSNGVPLWIIILAILFGLLLLGLLIYVLYKLGFFKRSLPYGTAMEKAQLKPPAT
  SDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010577
• ORF Size: 3159 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010577.4, NP_034707.5
• RefSeq Size: 4397 bp
• RefSeq ORF: 3162 bp
• Locus ID: 16402
• UniProt ID: P11688
• Cytogenetics: 15 58.9 cM
• MW: 115.5 kDa
• Gene Summary: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain, which is proteolytically processed to generate light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signaling. Integrin alpha 5 and integrin alpha V chains are produced by distinct genes. Homozygous knockout mice for this gene exhibit embryonic lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]