Bub1b (NM_009773) Mouse Tagged ORF Clone

CAT#: MR211540

Bub1b (Myc-DDK-tagged) - Mouse budding uninhibited by benzimidazoles 1 homolog, beta (S. cerevisiae) (Bub1b)

Product Images

Circular map for MR211540

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bub1b
• Synonyms: AU045529; BUBR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211540 representing NM_009773
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCGAGTGAAGCCATGTGCCTGGAGGGAGCAGAGTGGGAGCTGAGTAAAGAAAACATACAGC
  CCTTACGGCACGGGCGGGTCATGTCCACACTTCAGGGAGCTTTGGCAAAGCAAGAGTCAGCCGGCCACAC
  TGCTCTGCAGCAGCAGAAACGGGCATTTGAATCTGAAATCCGCTTTTACTCTGGAGATGACCCTCTGGAT
  GTGTGGGACAGATATATTAATTGGACAGAACAGAACTACCCTCAAGGGGGGAAAGAGAGTAACATGTCAG
  CGTTAGTGGAGAGAGCGATAGAAGCACTCCAAGGAGAGACGCGCTATTATAATGACCCCCGCTTTCTCAG
  TCTCTGGATCAAATTGGGACATTTGTGCAATGAACCTTTGGATATGTACAGCTATTTACAAAGCCAAGGA
  ATTGGCGTTTCCCTTGCCCAGTTCTATATTTCATGGGCTGAAGAATACGAAGCTAGAGAAAATTTCAAGA
  AAGCGGACATAATATTCCAGGAAGGGATTGAACGCAAGGCTGAGCCCCTGGACAGACTGCAGTCCCAGCA
  CAGACAGTTCCAGTCTCGAGTGTCGCGACAAGCTTTCTTGGCCCTTGGGAATGAAGAGGAGGAGGCTTTG
  GAGCCTTCTGAACCACAGAGAAGCTCGCTAGCTGAGCTGAAGAGCAGAGGGAAGAAGATGGCCAGAGCGC
  CCATCAGCCGTGTCGGAGGTGCTCTGAAAGCTCCAGGTCAGAGCAGAGGGTTCCTAAATGCAGTTCCCCA
  GCCAGTACACGGTAATCGCAGGATCACCGTTTTTGATGAAAATGCCGATACCGCGTCTAGAACGGAGTTA
  TCTAAGCCTGTAGCCCAGCCATGGATGGCACCCCCTGTGCCCAGGGCCAAAGAGAACGAACTTCAGCCAG
  GCCCATGGAGCACAGACAGGCCGGCGGGACGCAGGCCTCATGACAATCCAGCCTCTGTGACGTCGATTCC
  CAGCGTGCTTCCCAGCTTTACGCCGTACGTGGAAGAGAGCGCCCAGCAGACAGTCATGACACCATGCAAG
  ATTGAGCCTAGTATCAACCATGTTCTCAGCACCAGGAAGCCAGGGAGAGAAGAAGGAGACCCCTTGCAGA
  GAGTTCAGAGTCATCAGCAAGGCTGTGAGGAGAAGAAGGAGAAGATGATGTACTGTAAGGAGAAGATCTA
  TGCCGGAGTTGGGGAGTTCTCCTTTGAGGAGATCCGAGCTGAAGTGTTCCGAAAGAAGCTGAAAGAACGA
  AGGGAAGCCGAGCTGTTGACCAGTGCAAAGAAGAGGGAGGAGATGCAGAAGCAGATCGAAGAGATGGAGA
  GGAGGCTGAAGGCAATGCAGGCTGTTCAGCAAGAAGGAGCTGGGGGCCAGCAAGAAGAGAAGATGCCTAC
  AGAGGACCCAGCCAGATTGCAGATTGCTTCGGGGCCTCAGGAAATGTCGGGAGTTCCTCTGTCCTGTTCC
  ATCTGTCCACTAAGCTCGAATCCTAGGGAAATTTCACCTGCTGAGAACATTTTGCAAGAACAGCCTGATT
  CTAAAGGTTCCAGTATGCCTTTCTCCATTTTTGATGAGTCTCTTTCAGACAAAAAGGACAAAAGTCCTGC
  TACAGGTGGTCCACAGGTTCTCAATGCCCAGAGAAGACCCCTTTCAGTTCTCAAAACTACAGAAGTGGGC
  ACCACAAATGAGGATGTGTCTCCCGATATTTGTGATGAACTCACAGAACTTGAGCCTCTGAGTGAAGACG
  CCATCATCACTGGTTTCAGGAACGTCACTCTCTGTCCCAACCCTGAGGACACTTGTGACTTTGCTAGAGC
  AGCTCGTTTGGCATCTACTCCTTTCCATGAGATACTGTCCTCGAAGGGCATCGCTGCTGATCCCGAGGGA
  CTGTTGCAGGAAGAGGATCTGGATGGGAAGGCCGCCGAGGCTCATCACACTGTTCATCACCAGGCCCTCA
  TCATAAAGAAACTGAGCCCAATTATTGAAGAGAGCCGTGAGGCCACCCACTCATCTGGCTTCTCCAGGTC
  TTCTTCCTCAGCTCCCAGTACATCCTCCATCAAAGGCTTTCAGCTTCTGGAAAAGCTGGAGCTGACTAAT
  GACGGGGCAGAAGATGCTATTCAGTCACCCTGGTGTTCACAGTATCGCCTACAACTGTTAAAATCCCTAC
  TAGAATTAAGTGCTTTTGCGGAGTTTTCTGTGGAAGACCGACCGATGCCTGTGCTGGAAATAGGGAAGGA
  GATTGAGTTAGGTCCTGAGGATTACGTCATCAAGCAAGAGCACCTAACATGTGACGATTACAGGTTATTC
  TGGGTGGCACCAAGAAGCTCTGCAGAGCTAACCATGATAAAGGCATCATCTCAGCCTATCCCGTGGGATT
  TTTATATCAACCTCAAGTTGAAGGAGCGTCTGAATGAGGACTATGACCAGCTTTGCAGCTGCTGTCAGTA
  CCAAGATGGCCATGTTGTTTGGTACCAGTATATAAACTGCTCCACCCTTCAGAATCTTCTCCAACACAGC
  GAATTTGTTACTCATGAAATAATAGTGTTGATTATTTACAACCTCTTGACAATCGTGGAGAAGCTACACA
  GAGCTGAAATAGTACACGGAGACTTGAGTCCACGGAGTCTGATCCTACGAAACAGAATCCACGACCCCTA
  TGACTATGTAAATAAGGACGATCACGCTGTGAGGATCATGGACTTCTCCTACAGTGTTGACCTGAGGGTG
  CAGCTGGATGCGTTTGCCTATAGTGGCTTTCGGACTGCACAGATCCTGGAAGGACAAAAGATCCTGGCTA
  ACTGTTCTTCTCCCTACCATGTAGATCTGTTGGGTATAGCAGACCTAGCGCACTTACTCCTGTTCAAGGA
  GCACCTCCATGTCTTCTGGGATGGACTCCTCTGGAAACTTAGCCAGAGCACCTCTGAGCTAAAAGACAGT
  GAATTGTGGAATAAATTCTTTGTGCGGATTCTGAATGCCAGTGACAAGTCCACAGTGTCTGTTCTGGGGG
  AGCTGGCAGCAGAAATGGGTGGGGCTTTTGATGCCACATTCCATAGCCACCTGAACAGAGCCCTGTGGAA
  GCTGGGGAAGACAATCAGCCCGGAAGCTTTGCTCACTCAGCAAGACAAGCAGCCAGGCGGCTCCCAGAGC
  CCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211540 representing NM_009773
  Red=Cloning site Green=Tags(s)
  
  MAEASEAMCLEGAEWELSKENIQPLRHGRVMSTLQGALAKQESAGHTALQQQKRAFESEIRFYSGDDPLD
  VWDRYINWTEQNYPQGGKESNMSALVERAIEALQGETRYYNDPRFLSLWIKLGHLCNEPLDMYSYLQSQG
  IGVSLAQFYISWAEEYEARENFKKADIIFQEGIERKAEPLDRLQSQHRQFQSRVSRQAFLALGNEEEEAL
  EPSEPQRSSLAELKSRGKKMARAPISRVGGALKAPGQSRGFLNAVPQPVHGNRRITVFDENADTASRTEL
  SKPVAQPWMAPPVPRAKENELQPGPWSTDRPAGRRPHDNPASVTSIPSVLPSFTPYVEESAQQTVMTPCK
  IEPSINHVLSTRKPGREEGDPLQRVQSHQQGCEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKER
  REAELLTSAKKREEMQKQIEEMERRLKAMQAVQQEGAGGQQEEKMPTEDPARLQIASGPQEMSGVPLSCS
  ICPLSSNPREISPAENILQEQPDSKGSSMPFSIFDESLSDKKDKSPATGGPQVLNAQRRPLSVLKTTEVG
  TTNEDVSPDICDELTELEPLSEDAIITGFRNVTLCPNPEDTCDFARAARLASTPFHEILSSKGIAADPEG
  LLQEEDLDGKAAEAHHTVHHQALIIKKLSPIIEESREATHSSGFSRSSSSAPSTSSIKGFQLLEKLELTN
  DGAEDAIQSPWCSQYRLQLLKSLLELSAFAEFSVEDRPMPVLEIGKEIELGPEDYVIKQEHLTCDDYRLF
  WVAPRSSAELTMIKASSQPIPWDFYINLKLKERLNEDYDQLCSCCQYQDGHVVWYQYINCSTLQNLLQHS
  EFVTHEIIVLIIYNLLTIVEKLHRAEIVHGDLSPRSLILRNRIHDPYDYVNKDDHAVRIMDFSYSVDLRV
  QLDAFAYSGFRTAQILEGQKILANCSSPYHVDLLGIADLAHLLLFKEHLHVFWDGLLWKLSQSTSELKDS
  ELWNKFFVRILNASDKSTVSVLGELAAEMGGAFDATFHSHLNRALWKLGKTISPEALLTQQDKQPGGSQS
  PA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009773
• ORF Size: 3156 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009773.3, NP_033903.2
• RefSeq Size: 3669 bp
• RefSeq ORF: 3159 bp
• Locus ID: 12236
• UniProt ID: Q9Z1S0
• Cytogenetics: 2 E5
• MW: 118.8 kDa
• Gene Summary: Essential component of the mitotic checkpoint. Required for normal mitosis progression and tumor suppression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. Essential for tumor suppression. May play a role in regulating aging and fertility (By similarity).[UniProtKB/Swiss-Prot Function]