Epha1 (NM_023580) Mouse Tagged ORF Clone

CAT#: MR211365

Epha1 (Myc-DDK-tagged) - Mouse Eph receptor A1 (Epha1)

Product Images

Circular map for MR211365

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Epha1
• Synonyms: 5730453L17Rik; AL033318; Eph; Esk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211365 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCGCTGGCCCCTGGGGCTTGCATTGCTGCTGCTGCTGCTCTGCGCCCCGCTGCCCCCGGGGG
  CGCGCGCCGAGGAAGTCACTCTAATGGACACAAGCACAGCACAAGCAGAGCTGGGCTGGCTTCTGGATCC
  CCCAGAGACTGGGTGGAGTGAGGTGCAACAAATGCTAAACGGGACACCCCTGTACATGTACCAAGACTGC
  CCAATACAGGAAGGTGGGGACACTGACCACTGGCTTCGCTCCAATTGGATCTACCGCGGAGAGGAAGCTT
  CACGCATCTACGTGGAGCTGCAGTTCACCGTGCGGGACTGTAAGAGTTTCCCAGGGGGAGCTGGGCCTCT
  GGGATGCAAAGAGACCTTCAACCTTTTCTACATGGAGAGTGACCAGGATGTGGGCATTCAGCTCCGACGA
  CCTTTGTTCCAAAAGGTAACAACTGTGGCAGCAGACCAGAGCTTCACCATCAGAGACCTGGCATCTGGCT
  CTGTAAAGCTGAATGTAGAACGCTGCTCGTTGGGCCACCTCACCCGCCGTGGCCTCTACTTAGCTTTCCA
  CAACCCGGGTTCCTGTGTGGCGCTAGTGTCTGTAAGGGTGTTCTACCAGCGCTGTGCCGAGACCGTGCAT
  GGCTTGGCCCACTTCCCTGACACTCTCCCTGGACCTGGAGGGTTGGTTGAAGTAGCTGGAACGTGCCTCT
  CCCATGCACAGATCAGCTTGGGGTCCTCAGGTACACCACGAATGCACTGCAGCCCTGATGGCGAGTGGCT
  GGTGCCTGTGGGTCAGTGCCAGTGCGAGCCTGGCTATGAAGAAAGCAGTGGAAATGTGGGATGCACTGCC
  TGTCCTACTGGTTTCTATCGAGTGGACATGAATACACTCCGTTGTCTCAAGTGCCCCCAACATAGCATAG
  CAGAGTCTGAGGGGTCTACCATCTGTACCTGTGAGAATGGACATTATCGAGCCCCTGGGGAGGGTCCCCA
  GGTAGCATGCACACGTCCCCCATCGGCTCCCCAAAATCTGAGCTTCTCCACATCAGGGACTCAACTCTCC
  CTGCGCTGGGAGCCCCCCAGAGATACAGGGGGACGCCATGATATCAGATACAGCGTGGAGTGCTTGCAGT
  GTCGGGGCATTGCACAGGATGGGGGTCCCTGCCAACCCTGTGGAAAAGGTGTGCACTTTTCCCCGGCTGC
  TTCCGGGCTCACCACATCTACCGTGCAAGTGCAAGGCCTCGAGCCTTACGCCAACTACACATTTACCGTC
  AAATCCCAAAACAGAGTGTCAGGACTGGACAGTTCCAGCCCTAGCAGCGCCTCCCTCAGTATCAACATGG
  GGCACGCAGAGTCACTCTCTGGCCTGTCACTGAAGCTGGTGAAGAAAGAACCGAGGCAGCTGGAGCTGAC
  TTGGGCAGGGTCCCGACCCCGAAATCCTGGAGGGAATCTGAGCTATGAGCTGCACGTGCTGAATCAGGAC
  GAAGAATGGCACCAGATGGTGTTGGAACCCAGGGTCTTGCTGACAAAACTTCAGCCAGATACCACATACA
  TTGTCAGAGTGCGAACACTGACCCCACTGGGGCCTGGCCCTTTCTCCCCTGACCATGAGTTTCGGACAAG
  CCCACCAGTTTCCAGAAGCCTGACCGGAGGAGAGATTGTGGCCGTCATCTTTGGATTGCTGCTTGGAATA
  GCTCTGCTGATCGGGATTTATGTCTTCCGTTCAAGGAGAGGCCAGAGACAGAGACAGCAGAGGCAGCGTG
  AACGCACCACCAATGTCGATCGAGAGGACAAGCTGTGGCTAAAACCCTATGTGGACCTCCAGGCCTATGA
  GGACCCTGCACAGGGAGCCTTAGACTTTGCCCAGGAACTGGACCCAGCCTGGCTGATTGTGGACACTGTC
  ATAGGAGAAGGGGAGTTTGGTGAAGTGTATCGGGGAGCCCTGAGACTCCCCAGCCAAGATTGCAAGACTG
  TGGCCATTAAGACCTTGAAAGATACATCCCCAGATGGCTACTGGTGGAATTTCCTTCGAGAGGCAACTAT
  CATGGGCCAGTTCAACCACCCACACATTCTACGCCTAGAAGGTGTCATCACAAAAAGAAAGCCCATCATG
  ATCATCACAGAATTTATGGAAAATGGAGCCCTGGATGCCTTTCTGAAGGAACGGGAGGACCAACTAGCTC
  CTGGTCAGCTAGTGGCTATGCTACTGGGCATAGCATCAGGCATGAACTGCCTCAGTGGCCACAATTATGT
  CCATAGAGACCTGGCTGCCAGGAACATCTTGGTGAATCAGAACCTGTGCTGCAAGGTGTCTGACTTTGGC
  TTGACCCGCCTCCTGGATGACTTTGACGGCACCTATGAAACCCAGGGAGGAAAGATCCCCATCCGATGGA
  CAGCCCCAGAAGCTATTGCCCATCGGATCTTCACCACAGCCAGTGATGTGTGGAGCTTTGGGATTGTAAT
  GTGGGAGGTGTTGAGTTTTGGCGACAAACCCTATGGGGAGATGAGCAACCAAGAGGTAATGAAAAGCATT
  GAAGATGGGTACCGGTTGCCCCCTCCTGTGGACTGTCCTGCCCCTCTCTATGAACTCATGAAGAACTGCT
  GGGCTTACGATCGTGCCCGTCGACCCCACTTCCTCCAGCTGCAGGCACATCTGGAACAGTTGCTTACTGA
  CCCCCATTCCCTAAGGACAATTGCCAACTTTGACCCTAGGGTGACCTTACGCCTGCCCAGCCTGAGTGGC
  TCTGATGGGATCCCTTATCGAAGTGTCTCTGAGTGGCTTGAATCCATACGCATGAAGCGCTACATCCTGC
  ACTTCCGTTCGGCTGGGCTGGACACCATGGAGTGTGTGCTGGAGCTGACGGCTGAGGACCTGACGCAGAT
  GGGAATAACGTTGCCAGGGCACCAGAAACGAATTCTCTGCAGTATTCAAGGATTTAAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211365 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERRWPLGLALLLLLLCAPLPPGARAEEVTLMDTSTAQAELGWLLDPPETGWSEVQQMLNGTPLYMYQDC
  PIQEGGDTDHWLRSNWIYRGEEASRIYVELQFTVRDCKSFPGGAGPLGCKETFNLFYMESDQDVGIQLRR
  PLFQKVTTVAADQSFTIRDLASGSVKLNVERCSLGHLTRRGLYLAFHNPGSCVALVSVRVFYQRCAETVH
  GLAHFPDTLPGPGGLVEVAGTCLSHAQISLGSSGTPRMHCSPDGEWLVPVGQCQCEPGYEESSGNVGCTA
  CPTGFYRVDMNTLRCLKCPQHSIAESEGSTICTCENGHYRAPGEGPQVACTRPPSAPQNLSFSTSGTQLS
  LRWEPPRDTGGRHDIRYSVECLQCRGIAQDGGPCQPCGKGVHFSPAASGLTTSTVQVQGLEPYANYTFTV
  KSQNRVSGLDSSSPSSASLSINMGHAESLSGLSLKLVKKEPRQLELTWAGSRPRNPGGNLSYELHVLNQD
  EEWHQMVLEPRVLLTKLQPDTTYIVRVRTLTPLGPGPFSPDHEFRTSPPVSRSLTGGEIVAVIFGLLLGI
  ALLIGIYVFRSRRGQRQRQQRQRERTTNVDREDKLWLKPYVDLQAYEDPAQGALDFAQELDPAWLIVDTV
  IGEGEFGEVYRGALRLPSQDCKTVAIKTLKDTSPDGYWWNFLREATIMGQFNHPHILRLEGVITKRKPIM
  IITEFMENGALDAFLKEREDQLAPGQLVAMLLGIASGMNCLSGHNYVHRDLAARNILVNQNLCCKVSDFG
  LTRLLDDFDGTYETQGGKIPIRWTAPEAIAHRIFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSI
  EDGYRLPPPVDCPAPLYELMKNCWAYDRARRPHFLQLQAHLEQLLTDPHSLRTIANFDPRVTLRLPSLSG
  SDGIPYRSVSEWLESIRMKRYILHFRSAGLDTMECVLELTAEDLTQMGITLPGHQKRILCSIQGFKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023580
• ORF Size: 2934 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023580.1, NM_023580.2, NM_023580.3, NM_023580.4, NP_076069.2
• RefSeq Size: 3273 bp
• RefSeq ORF: 2934 bp
• Locus ID: 13835
• UniProt ID: Q60750
• Cytogenetics: 6 B2.1
• MW: 108.6 kDa
• Gene Summary: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.[UniProtKB/Swiss-Prot Function]