Anpep (NM_008486) Mouse Tagged ORF Clone

CAT#: MR211336

Anpep (Myc-DDK-tagged) - Mouse alanyl (membrane) aminopeptidase (Anpep)

Product Images

Circular map for MR211336

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Anpep
• Synonyms: AP-M; AP-N; Apn; Cd13; P150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211336 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGGGGTTCTACATTTCCAAGACCCTGGGCATCTTGGGCATCCTGTTGGGTGTGGCAGCTGTGT
  GTACCATCATAGCTCTGTCGGTGGTCTACGCTCAGGAGAAGAATAGGAATGCAGAGAACTCTGCCACAGC
  CCCCACGCTCCCGGGCAGCACCTCAGCCACCACCGCAACCACCACCCCTGCTGTAGATGAAAGCAAGCCT
  TGGAACCAGTATCGCTTGCCTAAGACTCTTATACCTGACTCCTACCGGGTGATCCTGAGACCCTACCTCA
  CCCCCAACAATCAGGGCCTGTACATCTTCCAAGGCAACAGTACTGTTCGCTTTACCTGCAACCAGACCAC
  GGATGTCATTATCATCCACAGCAAAAAGCTCAACTACACCCTCAAAGGAAACCACAGGGTGGTGTTGCGA
  ACCCTGGACGGCACTCCGGCACCTAACATTGACAAAACGGAACTGGTAGAGCGTACTGAGTACCTGGTGG
  TGCACCTGCAGGGGTCCCTGGTAGAGGGCCGTCAGTACGAGATGGACAGCCAGTTCCAGGGGGAACTGGC
  TGATGACCTGGCTGGCTTCTACCGCAGCGAGTACATGGAAGGAGACGTCAAGAAAGTGGTGGCTACAACG
  CAGATGCAGGCTGCTGATGCTCGGAAATCCTTTCCATGTTTTGATGAGCCAGCCATGAAGGCCATGTTCA
  ACATCACACTCATCTACCCCAACAACCTCATAGCTCTGTCTAATATGCTTCCCAAAGAGTCCAAGCCCTA
  TCCGGAAGACCCTTCCTGCACCATGACTGAGTTCCACTCCACCCCTAAGATGTCCACATACCTGCTGGCC
  TACATCGTGAGCGAGTTCAAAAATATAAGCTCCGTCTCAGCCAATGGTGTCCAGATTGGAATCTGGGCTC
  GGCCCAGTGCCATTGATGAGGGCCAGGGTGATTACGCACTGAACGTTACAGGCCCCATCCTAAATTTCTT
  TGCCCAACATTATAATACATCCTACCCTCTACCAAAGTCTGACCAGATTGCCCTGCCTGACTTCAACGCT
  GGAGCCATGGAGAACTGGGGTCTGGTGACCTACCGTGAGAGCTCCCTGGTCTTTGACTCTCAGTCCTCCT
  CCATTAGCAACAAGGAGCGGGTGGTCACTGTGATTGCTCACGAGCTGGCCCATCAGTGGTTTGGCAACCT
  GGTGACTGTGGCTTGGTGGAATGATCTGTGGCTGAACGAGGGCTTTGCCTCCTACGTGGAATATCTGGGT
  GCTGACTATGCAGAGCCTACCTGGAATCTGAAAGACCTCATGGTACTGAATGATGTGTACCGTGTGATGG
  CCGTGGATGCCCTTGCCTCCTCCCACCCACTGTCCAGTCCTGCTGACGAGATCAAAACACCAGACCAGAT
  CATGGAGCTGTTTGACAGCATCACCTACAGCAAGGGAGCCTCAGTCATCAGGATGCTGTCCAGTTTCCTG
  ACAGAGGACCTGTTTAAGAAGGGCCTTTCATCTTATCTCCACACCTACCAGTACTCGAACACCGTTTATC
  TGGACCTGTGGGAACACCTGCAAAAGGCCGTGAACCAACAGACAGCTGTCCAACCCCCGGCCACGGTGCG
  CACTATCATGGACCGCTGGATTCTACAGATGGGCTTTCCCGTTATCACTGTGAACACCAATACAGGAGAA
  ATCTCCCAGAAACACTTCCTCCTGGATTCCAAGTCCAACGTTACCCGCCCCTCCGAGTTTAATTACATCT
  GGATCGCGCCCATTCCATTTCTCAAAAGTGGACAGGAGGATCACTACTGGCTGGATGTCGAGAAAAACCA
  GAGTGCAAAGTTCCAGACATCCTCCAATGAATGGATCTTACTGAACATTAACGTAACCGGCTACTACCTG
  GTTAACTATGATGAGAACAACTGGAAGAAGCTTCAGAATCAGCTGCAAACAGACCTTTCTGTTATCCCTG
  TCATCAACCGAGCACAGATTATCCACGACTCCTTCAACCTGGCCAGTGCTAAAATGATACCCATCACCCT
  GGCGCTGGACAACACCCTCTTCCTGGTCAAAGAGGCGGAGTACATGCCCTGGCAGGCTGCCCTGAGCAGC
  CTCAACTACTTCACACTCATGTTCGACCGCTCGGAGGTCTACGGCCCCATGAAGAGGTATCTGAAGAAGC
  AAGTTACGCCCCTCTTCTTCTACTTCCAAAATAGAACCAACAACTGGGTCAACCGTCCTCCAACGCTGAT
  GGAGCAGTACAATGAAATTAACGCCATCAGCACCGCCTGTTCCAGTGGTCTCAAAGAGTGTAGGGACCTG
  GTCGTTGAGCTCTATAGTCAGTGGATGAAAAACCCTAATAATAACACGATCCACCCCAACCTTCGGTCTA
  CTGTCTACTGCAATGCCATTGCTTTCGGTGGCGAAGAAGAGTGGAACTTTGCTTGGGAACAGTTCCGGAA
  TGCAACTCTGGTGAACGAAGCGGACAAACTCCGGTCAGCCTTGGCCTGTAGCAAAGATGTGTGGATTTTG
  AACAGGTACCTGAGTTACACTCTGAACCCGGACTACATCCGGAAGCAGGACACCACCTCCACCATCATCA
  GCATTGCCAGCAACGTGGCTGGGCACCCTCTGGTTTGGGACTTTGTCCGAAGCAACTGGAAGAAACTGTT
  TGAGAATTACGGTGGAGGATCTTTCTCCTTTGCCAATCTCATCCAGGGAGTGACCCGGCGCTTCTCCTCT
  GAGTTCGAGCTGCAGCAGCTGGAGCAGTTTAAAGCGGATAACTCAGCCACAGGCTTTGGCACCGGCACTC
  GGGCTCTGGAGCAAGCCCTGGAGAAGACGAGAGCCAACATCGACTGGGTGAAGGAGAACAAAGATGCGGT
  ATTCAAGTGGTTCACAGAGAACAGCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211336 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKGFYISKTLGILGILLGVAAVCTIIALSVVYAQEKNRNAENSATAPTLPGSTSATTATTTPAVDESKP
  WNQYRLPKTLIPDSYRVILRPYLTPNNQGLYIFQGNSTVRFTCNQTTDVIIIHSKKLNYTLKGNHRVVLR
  TLDGTPAPNIDKTELVERTEYLVVHLQGSLVEGRQYEMDSQFQGELADDLAGFYRSEYMEGDVKKVVATT
  QMQAADARKSFPCFDEPAMKAMFNITLIYPNNLIALSNMLPKESKPYPEDPSCTMTEFHSTPKMSTYLLA
  YIVSEFKNISSVSANGVQIGIWARPSAIDEGQGDYALNVTGPILNFFAQHYNTSYPLPKSDQIALPDFNA
  GAMENWGLVTYRESSLVFDSQSSSISNKERVVTVIAHELAHQWFGNLVTVAWWNDLWLNEGFASYVEYLG
  ADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSSPADEIKTPDQIMELFDSITYSKGASVIRMLSSFL
  TEDLFKKGLSSYLHTYQYSNTVYLDLWEHLQKAVNQQTAVQPPATVRTIMDRWILQMGFPVITVNTNTGE
  ISQKHFLLDSKSNVTRPSEFNYIWIAPIPFLKSGQEDHYWLDVEKNQSAKFQTSSNEWILLNINVTGYYL
  VNYDENNWKKLQNQLQTDLSVIPVINRAQIIHDSFNLASAKMIPITLALDNTLFLVKEAEYMPWQAALSS
  LNYFTLMFDRSEVYGPMKRYLKKQVTPLFFYFQNRTNNWVNRPPTLMEQYNEINAISTACSSGLKECRDL
  VVELYSQWMKNPNNNTIHPNLRSTVYCNAIAFGGEEEWNFAWEQFRNATLVNEADKLRSALACSKDVWIL
  NRYLSYTLNPDYIRKQDTTSTIISIASNVAGHPLVWDFVRSNWKKLFENYGGGSFSFANLIQGVTRRFSS
  EFELQQLEQFKADNSATGFGTGTRALEQALEKTRANIDWVKENKDAVFKWFTENSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008486
• ORF Size: 2901 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008486.3
• RefSeq Size: 3439 bp
• RefSeq ORF: 2901 bp
• Locus ID: 16790
• UniProt ID: P97449
• Cytogenetics: 7 D2
• MW: 109.7 kDa
• Gene Summary: Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines (By similarity). May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells (PubMed:8691132). May have a role in angiogenesis and promote cholesterol crystallization (By similarity).[UniProtKB/Swiss-Prot Function]