Asap2 (NM_001098168) Mouse Tagged ORF Clone

CAT#: MR211308

Asap2 (Myc-DDK-tagged) - Mouse ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (Asap2), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Asap2
• Synonyms: 6530401G17Rik; Ddef2; Gm592; Gm1523; PAG3; PAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211308 representing NM_001098168
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAGGACTACAAGGCGCCCACGGCCT
  CTAGCTTCACCACGCGCACGGCCCAGTGCCGGAACACCGTGGCGGCCATCGAGGAGGCTTTGGACGTGGA
  CCGGATGGTTCTCTACAAAATGAAGAAATCTGTGAAGGCAATCAATATCTCTGGGCTGGCACACGTGGAG
  AACGAGGAACAGTACACCCAGGCTCTGGAGAAGTTCGGTGGCAACTGTGTGTGCAGAGATGATCCAGACT
  TAGGAAGCGCGTTTCTGAAGTTCTCTGTGTTCACCAAGGAGTTGACGGCACTCTTCAAAAACCTGATTCA
  GAACATGAACAACATCATCTCGTTCCCTTTGGACAGTTTGCTGAAAGGAGATCTGAAAGGAGTAAAAGGG
  GATCTGAAAAAGCCCTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAAATAGAAAAGGAGA
  AAAAGGAGCATGCCAAGCTCCACGGGATGATTCGTACTGAAATAAGCGGGGCTGAGATTGCAGAGGAGAT
  GGAGAAGGAAAGACGGTTCTTCCAGCTGCAGATGTGTGAGTACCTGCTGAAGGTCAATGAAATCAAGGTG
  AAAAAGGGAGTGGATTTGCTTCAGAATCTGATCAAGTACTTTCACGCCCAGTGCAATTTTTTCCAGGATG
  GATTGAAAGCAGTAGAAAGCCTCAAGCCTTCCATCGAGACGCTCTCCACGGACCTCCACACCATCAAACA
  GGCCCAGGATGAGGAACGGCGACAGCTGATACAACTTCGAGATATTTTGAAGTCAGCATTGCAGGTGGAA
  CAGAAAGAGTCTAGGAGAGACTCACAACTTCGCCAAAGTACAGCCTACAGCTTACATCAACCTCAGGGAA
  ACAAGGAGCATGGAACAGAGAGGAACGGGAACCTCTACAAGAAGAGCGATGGGATCCGGAAAGTGTGGCA
  GAAGAGGAAGTGTTCCGTTAAAAATGGCTTCCTCACCATCTCCCACGGCACTGCCAACCGGCCACCTGCC
  AAGCTCAACCTGCTAACCTGCCAGGTGAAGACCAATCCTGAGGAGAAGAAGTGTTTCGACCTCATATCAC
  ATGACAGGACGTACCACTTCCAAGCGGAAGACGAACAAGAATGTCAGATATGGATGTCTGTACTGCAGAA
  CAGCAAGGAAGAAGCTCTGAACAACGCCTTTAAGGGTGATGACAACACTGGAGAAAATAACATCGTCCAA
  GAGCTGACCAAGGAGATCATCTCGGAGGTGCAGAGGATGACGGGCAATGACGTGTGCTGCGACTGTGGGG
  CACCAGATCCGACGTGGCTCTCTACCAACCTGGGCATCCTGACTTGCATCGAGTGCTCTGGGATCCACCG
  GGAGCTGGGGGTTCATTACTCCAGGATGCAGTCCCTGACATTAGATGTATTGGGAACGTCTGAGCTTCTG
  CTTGCCAAGAATATCGGGAATGCAGGCTTTAATGAAATTATGGAGTGTTGCCTACCGTCTGAGGACCCAG
  TCAAGCCCAACCCAGGCAGTGACATGATTGCAAGGAAGGACTACATCACAGCCAAGTACATGGAGAGGAG
  ATATGCACGGAAAAAGCATGCCGACACCGCGGCGAAGCTCCACAGCCTTTGCGAGGCCGTCAAGACCAGA
  GACATTTTTGGGTTACTCCAAGCTTATGCTGATGGTGTGGACCTGACAGAGAAAATCCCACTGGCCAATG
  GGCATGAGCCAGATGAGACAGCCCTCCATCTTGCAGTCAGATCTGTGGACCGGACTTCCCTTCACATTGT
  AGACTTCCTGGTCCAGAACAGTGGGAACCTGGATAAACAGACAGGCAAGGGCAGCACAGCCCTGCACTAC
  TGCTGCCTGACAGACAATGCTGAGTGCCTCAAGCTCCTCCTGCGGGGAAAAGCCTCCATTGAGATAGCCA
  ATGAGTCAGGAGAGACCCCGTTGGACATTGCTAAGCGGCTCAAGCACGAACACTGTGAGGAACTGCTCAC
  TCAGGCCTTGTCGGGGCGGTTTAACTCCCATGTTCACGTCGAGTATGAATGGCGGCTGCTGCACGAGGAC
  CTGGATGAGAGCGACGACGATGTGGACGAGAAGCTACAGCCTAGTCCCAACCGACGGGAGGACCGGCCTG
  TTAGCTTCTACCAGCTGGGGTCCAGCCAGTTTCAGTCCAATGCTGTGTCTTTGGCCAGAGATACTGCAAA
  CCTCACCAAGGACAAGCAGAGGGGCTTTGGGCCCAGCATCTTGCAGAATGAGACCTATGGAGCCATCCTG
  AGTGGCAGCCCGCCCTCCTCCCAGTCAATACCCCCCAGCACCACCAGCGCACCCCCGCTCCCACCTCGGA
  ATGTTGGCAAAGATCCCCTGACCACCACACCACCCCCTCCTGTGGCTAAGACATCAGGAACTCTAGAAGC
  CATGAACCAGCCGAGCAAGTCCTCCCAACCTGGGACCTCACAGAGCAAGCCCCCGCCCCTGCCACCTCAG
  CCACCCAGCCGCCTGCCTCAGAAGAAACCAGCTTCCGGGACTGACAAGCCAACCCCGCTGACCAACAAAG
  GACAGCCGAGAGGACCTGAAGCTTCGGGTCCTCTGTCCAACGCCATGGCCCTGCAGCCCCCAGCACCCAT
  GCCTAGGAAGTCACAGGCGACCAAGTCAAAGCCCAAGCGAGTGAAAGCACTCTACAACTGCGTGGCTGAC
  AACCCAGACGAGCTGACTTTCTCTGAGGGCGACGTGATCATTGTGGATGGGGAAGAGGATCAGGAGTGGT
  GGATCGGCCACATCGATGGAGAGCCCAGCCGCAAAGGAGCGTTTCCCGTATCGTTTGTGCACTTTATTGC
  TGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211308 representing NM_001098168
  Red=Cloning site Green=Tags(s)
  
  MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEALDVDRMVLYKMKKSVKAINISGLAHVE
  NEEQYTQALEKFGGNCVCRDDPDLGSAFLKFSVFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKG
  DLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKV
  KKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSALQVE
  QKESRRDSQLRQSTAYSLHQPQGNKEHGTERNGNLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPA
  KLNLLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQ
  ELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELL
  LAKNIGNAGFNEIMECCLPSEDPVKPNPGSDMIARKDYITAKYMERRYARKKHADTAAKLHSLCEAVKTR
  DIFGLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHY
  CCLTDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHED
  LDESDDDVDEKLQPSPNRREDRPVSFYQLGSSQFQSNAVSLARDTANLTKDKQRGFGPSILQNETYGAIL
  SGSPPSSQSIPPSTTSAPPLPPRNVGKDPLTTTPPPPVAKTSGTLEAMNQPSKSSQPGTSQSKPPPLPPQ
  PPSRLPQKKPASGTDKPTPLTNKGQPRGPEASGPLSNAMALQPPAPMPRKSQATKSKPKRVKALYNCVAD
  NPDELTFSEGDVIIVDGEEDQEWWIGHIDGEPSRKGAFPVSFVHFIAD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098168
• ORF Size: 2874 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098168.1, NP_001091637.1
• RefSeq Size: 5566 bp
• RefSeq ORF: 2877 bp
• Locus ID: 211914
• UniProt ID: Q7SIG6
• Cytogenetics: 12 A1.2-A1.3
• MW: 107.3 kDa
• Gene Summary: Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration (By similarity).[UniProtKB/Swiss-Prot Function]