Adamts15 (NM_001024139) Mouse Tagged ORF Clone

CAT#: MR211283

Adamts15 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 15 (Adamts15)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adamts15
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211283 representing NM_001024139
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCTACTGGGCATCTCAATCCTGGCTCTCGCCTGGCGACCTGCTGGCAGCTCCGAGCCAGAGTGGG
  AGGTGGTCGTTCCAATCCGACGGGACCCGGACATCAATGGCCGCCACTACTACAGGAGGGGTACGGAGGA
  CTCCGGGGATCAGGGTCTCATTTTTCAGATCACAGCTTTTCAGCAGGACTTTTATCTACACCTGACGCCA
  GATGCCCAGTTCCTGGCTCCCGCCTTTGCTACTGAGTATCTAGGTGTCCCGCTGCAGAGGCTCACTGGCA
  GCTCTCTAGACCTGCGACGCTGCTTCTATTCCGGGTATGTGAACGCGGAGCCAGACTCCTTTGCTGCTGT
  GAGCCTATGCGGGGGTCTCCGCGGAGCCTTTGGCTACCGAGGTGCGGAGTATGTCATTAGCCCTCTGCCC
  AACACCAGCGCGCCAGAGGCGCAGCGTCACAGCCAGGGCGCACACCTTCTCCAGCGCCGGGGTGCTCCTG
  TAGGGCCTTCCGGAGACCCCACCTCTCGTTGCGGGGTGGCCTCGGGCTGGAACCCCGCCATCCTGAGGGC
  TCTGGACCCTTATAAGCCACGGCGGACGGGCGCGGGGGAGAGCCACAACCGCCGCAGGTCTGGGCGCGCC
  AAGCGCTTCGTGTCTATACCACGGTACGTGGAGACACTGGTGGTGGCGGACGAGTCAATGGTCAAGTTTC
  ACGGCGCGGATTTGGAACATTATCTACTGACGCTGCTGGCCACGGCGGCGCGACTCTACCGCCACCCCAG
  CATCCTCAACCCTATCAACATCGTGGTGGTCAAGGTGCTACTCTTAGGAGATCGTGACACTGGGCCCAAG
  GTCACAGGCAACGCGGCCCTGACTCTGCGCAACTTCTGTGCCTGGCAGAAAAAGCTGAACAAAGTGAGCG
  ATAAGCACCCTGAGTACTGGGACACAGCCATCCTCTTCACCAGACAGGACCTATGCGGGGCTACCACCTG
  TGACACCTTGGGTATGGCTGATGTGGGCACCATGTGTGACCCCAAGAGAAGCTGCTCTGTCATTGAGGAC
  GATGGGCTTCCATCAGCCTTCACCACTGCCCATGAGCTGGGCCATGTGTTCAACATGCCCCATGACAACG
  TGAAGGTGTGTGAGGAGGTGTTTGGGAAGCTCAGAGCCAACCACATGATGTCTCCGACCCTCATCCAGAT
  TGACCGTGCCAACCCCTGGTCAGCCTGCAGCGCTGCCATTATTACTGACTTCCTGGATAGTGGGCATGGT
  GACTGCCTCCTGGACCAGCCCAGCAAGCCCATCACCCTGCCTGAGGACCTGCCGGGCACAAGCTACAGTT
  TGAGCCAGCAGTGCGAGCTGGCCTTTGGGGTGGGCTCTAAGCCCTGCCCATATATGCAGTACTGTACAAA
  GCTGTGGTGCACTGGCAAGGCCAAGGGGCAGATGGTGTGCCAGACTCGCCACTTCCCCTGGGCAGATGGC
  ACCAGCTGTGGGGAAGGCAAGTTCTGCCTCAAGGGAGCCTGCGTGGAGAGACACAACCCAAACAAGTACC
  GGGTGGACGGCTCTTGGGCCAAGTGGGAGCCCTACGGTTCCTGCTCCCGCACCTGCGGTGGGGGCGTGCA
  GCTGGCCCGGAGGCAGTGCAGCAACCCTACCCCTGCCAACGGTGGAAAATACTGCGAGGGAGTAAGAGTG
  AAATACCGATCTTGCAACTTGGAGCCCTGCCCCAGCTCAGCCTCTGGCAAGAGCTTCCGGGAAGAGCAAT
  GTGAAGCTTTCAATGGCTACAACCACAGCACCAACCGGCTCACTTTAGCTGTGGCATGGGTACCCAAGTA
  CTCAGGCGTGTCACCACGTGACAAGTGTAAGCTCATCTGCCGAGCCAATGGGACTGGCTACTTCTATGTA
  CTAGCACCTAAGGTGGTGGATGGTACGCTGTGTACTCCTGACTCCACCTCGGTCTGTGTCCAAGGCAAGT
  GCATCAAGGCTGGCTGCGACGGGAATCTGGGCTCCAAGAAGAAATTTGACAAATGTGGTGTGTGTGGTGG
  AGACAATAAGAGCTGTAAGAGGGTGACAGGACTCTTCACCAAGCCTATGCACGGCTACAATTTTGTAGTG
  GCCATCCCTGCCGGCGCCTCCAGCATTGATATCCGTCAGCGTGGTTACAAGGGGCTCATTGGAGATGACA
  ACTACCTGGCTTTGAAGAACAGCCAAGGCAAATACCTGCTCAATGGGCACTTTGTGGTATCCGCTGTAGA
  GCGGGACCTGGTGGTAAAAGGCAGTGTGCTACGCTATAGTGGCACTGGCACTGCAGTGGAGAGCCTGCAG
  GCTTCTCGACCCATCCTGGAGCCACTCACCGTGGAGGTCCTGTCGGTGGGGAAGATGACACCGCCCCGTG
  TGCGTTATTCTTTCTACCTGCCCAAAGAACCTCGGGAGGACAAGTCCACTCGCCCCAAGGACCCTCGAGG
  TTCCCCCGTGTTGCGCAACAGTGTTCTCAGCCTTTCCAACCAAGTAGAGCAGCCAGATAACAGACCTCCT
  GCGCGCTGGGTGGCAGGCAGCTGGGGACCTTGCTCAGTGAGCTGTGGCAGTGGCCTACAGAAGCGAGCAG
  TGGACTGCCGGGACTCCCCAGGGCAGCAAGGAGCCTCTGCTTGTGATGTAGACCATCGGCCATTGGAGAA
  GCGAGCCTGTGGGGAACCCTGCCCAACTTGGGAGCTCGGCAACTGGTCGCCCTGTTCTAAAAGCTGTGGC
  CGTGGATTTAAGAGGCGTCCGCTCAAGTGTGTGGGCCACGGAGGCAGGCTGCTGGCTCGGGACCAGTGTG
  ACTTACGCCGCAAGCCCCAGGAATTAGACTTCTGCGTCTTGAGACCCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211283 representing NM_001024139
  Red=Cloning site Green=Tags(s)
  
  MLLLGISILALAWRPAGSSEPEWEVVVPIRRDPDINGRHYYRRGTEDSGDQGLIFQITAFQQDFYLHLTP
  DAQFLAPAFATEYLGVPLQRLTGSSLDLRRCFYSGYVNAEPDSFAAVSLCGGLRGAFGYRGAEYVISPLP
  NTSAPEAQRHSQGAHLLQRRGAPVGPSGDPTSRCGVASGWNPAILRALDPYKPRRTGAGESHNRRRSGRA
  KRFVSIPRYVETLVVADESMVKFHGADLEHYLLTLLATAARLYRHPSILNPINIVVVKVLLLGDRDTGPK
  VTGNAALTLRNFCAWQKKLNKVSDKHPEYWDTAILFTRQDLCGATTCDTLGMADVGTMCDPKRSCSVIED
  DGLPSAFTTAHELGHVFNMPHDNVKVCEEVFGKLRANHMMSPTLIQIDRANPWSACSAAIITDFLDSGHG
  DCLLDQPSKPITLPEDLPGTSYSLSQQCELAFGVGSKPCPYMQYCTKLWCTGKAKGQMVCQTRHFPWADG
  TSCGEGKFCLKGACVERHNPNKYRVDGSWAKWEPYGSCSRTCGGGVQLARRQCSNPTPANGGKYCEGVRV
  KYRSCNLEPCPSSASGKSFREEQCEAFNGYNHSTNRLTLAVAWVPKYSGVSPRDKCKLICRANGTGYFYV
  LAPKVVDGTLCTPDSTSVCVQGKCIKAGCDGNLGSKKKFDKCGVCGGDNKSCKRVTGLFTKPMHGYNFVV
  AIPAGASSIDIRQRGYKGLIGDDNYLALKNSQGKYLLNGHFVVSAVERDLVVKGSVLRYSGTGTAVESLQ
  ASRPILEPLTVEVLSVGKMTPPRVRYSFYLPKEPREDKSTRPKDPRGSPVLRNSVLSLSNQVEQPDNRPP
  ARWVAGSWGPCSVSCGSGLQKRAVDCRDSPGQQGASACDVDHRPLEKRACGEPCPTWELGNWSPCSKSCG
  RGFKRRPLKCVGHGGRLLARDQCDLRRKPQELDFCVLRPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024139
• ORF Size: 2850 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024139.1, NP_001019310.1
• RefSeq Size: 5946 bp
• RefSeq ORF: 2853 bp
• Locus ID: 235130
• UniProt ID: P59384
• Cytogenetics: 9 A4
• MW: 104.4 kDa
• Gene Summary: This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active versicanase enzyme. This gene is located adjacent to a related ADAMTS gene (Adamts8) on chromosome 9. [provided by RefSeq, Jul 2016]