Adamts15 (NM_001024139) Mouse Tagged ORF Clone

CAT#: MR211283

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  • TrueORF®

Adamts15 (Myc-DDK-tagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 15 (Adamts15)



  "NM_001024139" in other vectors (4)

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USD 750.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Adamts15 (NM_001024139) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Adamts15
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR211283 representing NM_001024139
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTCTACTGGGCATCTCAATCCTGGCTCTCGCCTGGCGACCTGCTGGCAGCTCCGAGCCAGAGTGGG
AGGTGGTCGTTCCAATCCGACGGGACCCGGACATCAATGGCCGCCACTACTACAGGAGGGGTACGGAGGA
CTCCGGGGATCAGGGTCTCATTTTTCAGATCACAGCTTTTCAGCAGGACTTTTATCTACACCTGACGCCA
GATGCCCAGTTCCTGGCTCCCGCCTTTGCTACTGAGTATCTAGGTGTCCCGCTGCAGAGGCTCACTGGCA
GCTCTCTAGACCTGCGACGCTGCTTCTATTCCGGGTATGTGAACGCGGAGCCAGACTCCTTTGCTGCTGT
GAGCCTATGCGGGGGTCTCCGCGGAGCCTTTGGCTACCGAGGTGCGGAGTATGTCATTAGCCCTCTGCCC
AACACCAGCGCGCCAGAGGCGCAGCGTCACAGCCAGGGCGCACACCTTCTCCAGCGCCGGGGTGCTCCTG
TAGGGCCTTCCGGAGACCCCACCTCTCGTTGCGGGGTGGCCTCGGGCTGGAACCCCGCCATCCTGAGGGC
TCTGGACCCTTATAAGCCACGGCGGACGGGCGCGGGGGAGAGCCACAACCGCCGCAGGTCTGGGCGCGCC
AAGCGCTTCGTGTCTATACCACGGTACGTGGAGACACTGGTGGTGGCGGACGAGTCAATGGTCAAGTTTC
ACGGCGCGGATTTGGAACATTATCTACTGACGCTGCTGGCCACGGCGGCGCGACTCTACCGCCACCCCAG
CATCCTCAACCCTATCAACATCGTGGTGGTCAAGGTGCTACTCTTAGGAGATCGTGACACTGGGCCCAAG
GTCACAGGCAACGCGGCCCTGACTCTGCGCAACTTCTGTGCCTGGCAGAAAAAGCTGAACAAAGTGAGCG
ATAAGCACCCTGAGTACTGGGACACAGCCATCCTCTTCACCAGACAGGACCTATGCGGGGCTACCACCTG
TGACACCTTGGGTATGGCTGATGTGGGCACCATGTGTGACCCCAAGAGAAGCTGCTCTGTCATTGAGGAC
GATGGGCTTCCATCAGCCTTCACCACTGCCCATGAGCTGGGCCATGTGTTCAACATGCCCCATGACAACG
TGAAGGTGTGTGAGGAGGTGTTTGGGAAGCTCAGAGCCAACCACATGATGTCTCCGACCCTCATCCAGAT
TGACCGTGCCAACCCCTGGTCAGCCTGCAGCGCTGCCATTATTACTGACTTCCTGGATAGTGGGCATGGT
GACTGCCTCCTGGACCAGCCCAGCAAGCCCATCACCCTGCCTGAGGACCTGCCGGGCACAAGCTACAGTT
TGAGCCAGCAGTGCGAGCTGGCCTTTGGGGTGGGCTCTAAGCCCTGCCCATATATGCAGTACTGTACAAA
GCTGTGGTGCACTGGCAAGGCCAAGGGGCAGATGGTGTGCCAGACTCGCCACTTCCCCTGGGCAGATGGC
ACCAGCTGTGGGGAAGGCAAGTTCTGCCTCAAGGGAGCCTGCGTGGAGAGACACAACCCAAACAAGTACC
GGGTGGACGGCTCTTGGGCCAAGTGGGAGCCCTACGGTTCCTGCTCCCGCACCTGCGGTGGGGGCGTGCA
GCTGGCCCGGAGGCAGTGCAGCAACCCTACCCCTGCCAACGGTGGAAAATACTGCGAGGGAGTAAGAGTG
AAATACCGATCTTGCAACTTGGAGCCCTGCCCCAGCTCAGCCTCTGGCAAGAGCTTCCGGGAAGAGCAAT
GTGAAGCTTTCAATGGCTACAACCACAGCACCAACCGGCTCACTTTAGCTGTGGCATGGGTACCCAAGTA
CTCAGGCGTGTCACCACGTGACAAGTGTAAGCTCATCTGCCGAGCCAATGGGACTGGCTACTTCTATGTA
CTAGCACCTAAGGTGGTGGATGGTACGCTGTGTACTCCTGACTCCACCTCGGTCTGTGTCCAAGGCAAGT
GCATCAAGGCTGGCTGCGACGGGAATCTGGGCTCCAAGAAGAAATTTGACAAATGTGGTGTGTGTGGTGG
AGACAATAAGAGCTGTAAGAGGGTGACAGGACTCTTCACCAAGCCTATGCACGGCTACAATTTTGTAGTG
GCCATCCCTGCCGGCGCCTCCAGCATTGATATCCGTCAGCGTGGTTACAAGGGGCTCATTGGAGATGACA
ACTACCTGGCTTTGAAGAACAGCCAAGGCAAATACCTGCTCAATGGGCACTTTGTGGTATCCGCTGTAGA
GCGGGACCTGGTGGTAAAAGGCAGTGTGCTACGCTATAGTGGCACTGGCACTGCAGTGGAGAGCCTGCAG
GCTTCTCGACCCATCCTGGAGCCACTCACCGTGGAGGTCCTGTCGGTGGGGAAGATGACACCGCCCCGTG
TGCGTTATTCTTTCTACCTGCCCAAAGAACCTCGGGAGGACAAGTCCACTCGCCCCAAGGACCCTCGAGG
TTCCCCCGTGTTGCGCAACAGTGTTCTCAGCCTTTCCAACCAAGTAGAGCAGCCAGATAACAGACCTCCT
GCGCGCTGGGTGGCAGGCAGCTGGGGACCTTGCTCAGTGAGCTGTGGCAGTGGCCTACAGAAGCGAGCAG
TGGACTGCCGGGACTCCCCAGGGCAGCAAGGAGCCTCTGCTTGTGATGTAGACCATCGGCCATTGGAGAA
GCGAGCCTGTGGGGAACCCTGCCCAACTTGGGAGCTCGGCAACTGGTCGCCCTGTTCTAAAAGCTGTGGC
CGTGGATTTAAGAGGCGTCCGCTCAAGTGTGTGGGCCACGGAGGCAGGCTGCTGGCTCGGGACCAGTGTG
ACTTACGCCGCAAGCCCCAGGAATTAGACTTCTGCGTCTTGAGACCCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR211283 representing NM_001024139
Red=Cloning site Green=Tags(s)

MLLLGISILALAWRPAGSSEPEWEVVVPIRRDPDINGRHYYRRGTEDSGDQGLIFQITAFQQDFYLHLTP
DAQFLAPAFATEYLGVPLQRLTGSSLDLRRCFYSGYVNAEPDSFAAVSLCGGLRGAFGYRGAEYVISPLP
NTSAPEAQRHSQGAHLLQRRGAPVGPSGDPTSRCGVASGWNPAILRALDPYKPRRTGAGESHNRRRSGRA
KRFVSIPRYVETLVVADESMVKFHGADLEHYLLTLLATAARLYRHPSILNPINIVVVKVLLLGDRDTGPK
VTGNAALTLRNFCAWQKKLNKVSDKHPEYWDTAILFTRQDLCGATTCDTLGMADVGTMCDPKRSCSVIED
DGLPSAFTTAHELGHVFNMPHDNVKVCEEVFGKLRANHMMSPTLIQIDRANPWSACSAAIITDFLDSGHG
DCLLDQPSKPITLPEDLPGTSYSLSQQCELAFGVGSKPCPYMQYCTKLWCTGKAKGQMVCQTRHFPWADG
TSCGEGKFCLKGACVERHNPNKYRVDGSWAKWEPYGSCSRTCGGGVQLARRQCSNPTPANGGKYCEGVRV
KYRSCNLEPCPSSASGKSFREEQCEAFNGYNHSTNRLTLAVAWVPKYSGVSPRDKCKLICRANGTGYFYV
LAPKVVDGTLCTPDSTSVCVQGKCIKAGCDGNLGSKKKFDKCGVCGGDNKSCKRVTGLFTKPMHGYNFVV
AIPAGASSIDIRQRGYKGLIGDDNYLALKNSQGKYLLNGHFVVSAVERDLVVKGSVLRYSGTGTAVESLQ
ASRPILEPLTVEVLSVGKMTPPRVRYSFYLPKEPREDKSTRPKDPRGSPVLRNSVLSLSNQVEQPDNRPP
ARWVAGSWGPCSVSCGSGLQKRAVDCRDSPGQQGASACDVDHRPLEKRACGEPCPTWELGNWSPCSKSCG
RGFKRRPLKCVGHGGRLLARDQCDLRRKPQELDFCVLRPC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_001024139
ORF Size 2850 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001024139.1, NP_001019310.1
RefSeq Size 5946 bp
RefSeq ORF 2853 bp
Locus ID 235130
Cytogenetics 9 A4
MW 104.4 kDa
Gene Summary This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active versicanase enzyme. This gene is located adjacent to a related ADAMTS gene (Adamts8) on chromosome 9. [provided by RefSeq, Jul 2016]

Citations (2)

Other Versions

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