OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Smarcal1 (NM_018817) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR211150
Smarcal1 (Myc-DDK-tagged) - Mouse SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (Smarcal1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $833.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Smarcal1 or NM_018817"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Smarcal1
Synonyms 6030401P21Rik; AI851716; Mharp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211150 representing NM_018817
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTTGCCACTTACAGAGGAGCAGAGGAAAAAGATTGAAGAGAATCGGCAAAAAGCTTTGGCCCGTC
GAGCTGAGAAGTTGTCAGAACAGCCACAGAGCGCAGCTTCAGGCTCCTCTGCTGCCGGCCCGTCCCAGTC
CAAGCAGGGCTCTCTCCTGAATCTCCTAGCGGAGCCTTCAAAGCCAGTGGGCCATGCCTCCATTTTCAAG
CAACAGAATCTCAGTAATTCATTTCCTACTGATCAGAGACCTCACAGCTCCCGCTGTTCTCAGCCCAGCC
CAGCAGAGGAAACGACGGGGCTATGGAAGACCCAAGGAGAGATGTCCACAGCCTGCCCAAAGCCTAACCC
AAGTCCTCCAGGAGCCTCTAACCAACCACTTTTGGGTTATAAATCCAGCGAGGGTCAACCCCAGGCAACC
TGGGACACAGGAGCTTCCTCTTCTGGACCATTCCCCAGGGACCCCGAGTTAGAGGCCAAGGCAGCAAGGC
CTTCCACCTCTAGGCAGAGCATTTCGGACAGCTTTTATGTGTTAGGGGGAAAAACACCTAGGACAGAAGG
GAGACCCCCAAACATACTGCAAACCACACCCCAAAACACAGGCTTTCTACGGGGAGCGTGCATAAAGACT
GGGGATCGCTTCCGGGTAAAGATCGGCTACAACCAAGAGCTCATTGCCGTGTTTAAGTCTCTGCCCAGCA
GACACTATGATTCTTTTACAAAGACCTGGGACTTCAGCATGAGCGACTACAGAGCCTTGATGAAGGCAGT
CGAGCGACTCTCCACGGTCTCCCTGAAGCCACTGGACGAGGCTGGTGGCAGTGTTGGAGGACAGACCAGC
CTTCCCTCAGCTCCATCCCTGACCTTTGTGACAGGGAAGTGCATGCTCATCTCTCGAGTCCGCTTCGAGG
TTGACATCGGCTATTCGGAGGCGGTGATTGGCCTGTTTAAACAGATGGAGTCCAGGAGTTACGATATCAA
GACCAGGAAGTGGAGTTTTCTCTTGGAAGAGCACAATAAACTAATTGCAAGGTCACGTGAACTGAAGCAA
GTTCAGCTGGACCCACTGCCCAAGACAGTGACCCTGGCATTTGCATCTCAGCTGGAGAAAACGTCTCCCA
AACTGAAAGCAGACGTGCCAGAAGCGGACCTTTCCGGAGTGGATGCCAAGCTTGTGTCTAGCCTCATGCC
CTTTCAGAGGGAGGGAGTTAGCTTTGCCATATCAAAAAGAGGCCGCCTGCTACTTGCCGATGACATGGGC
CTAGGGAAGACCGTCCAAGCCATCTGCATAGCAGCTTTTTACCGAAAGGAATGGCCACTGCTGGTGGTCG
TGCCTTCCTCTGTGCGCTTCACCTGGGAGCAGGCCTTTCTTCGGTGGCTTCCATCTCTTAGCCCAGAGAA
CATCAACGTGGTGGTGACCGGGAAGGGCCGCCTAACAGCTGGCTTGGTCAATATTGTCAGCTTTGACCTC
CTTTGCAAGTTGGAGAGGCAGCTAAAAACCCCTTTCAAAGTCGTCATTATTGATGAATCACACTTCCTCA
AGAACATAAAGACTGCCCGCTGCCGAGCAGCTGTGCCTATCCTAAAGGTTGCCAAGAGGGTGATCCTACT
GTCAGGCACACCCGCCATGTCTCGGCCCGCAGAGCTCTACACGCAGATCATTGCTGTCAAGCCAACCTTC
TTCCCTCAGTTTCATGCCTTTGGACTCCGCTACTGTGACGCCAAACGGCTCCCTTGGGGCTGGGACTACT
CAGGCTCCTCCAACCTGGGCGAGCTGAAGCTGCTGCTAGAGGAAGCAATCATGCTGCGGCGCCTCAAATC
CGACGTGCTCTCCCAGCTTCCGGCCAAGCAGCGCAAGATGGTGGTGGTCAACCCTGGTCGGATCAGCTCC
AGGGCTAAGGCAGCCCTGGATGCAGCAGCCAAAGAAATGACCAAGGACAAAACTAAACAGCAACAGAAAG
AAGCTCTCCTTGTCTTCTTCAACAGAACAGCAGAAGCTAAAATCCCATGTGTCGTCGAGTATATCCTGGA
CCTTCTGGACAGCGGACGAGAGAAGTTTCTAGTGTTTGCACACCATAAGGTGATTCTGGATGCAGTTGCC
AAGGAGCTCGAGAGGAAGAACGTTCAACACATCCGGATCGATGGCTCCACTCCCTCAGCAGACCGGGAGG
CTCAGTGCCAGCGGTTCCAGCTGTCCAAAGGGCACACAGTGGCACTGTTGTCCATCACAGCCGCCAACAT
GGGCCTCACCTTCTCCACAGCTGATCTGGTCGTGTTTGCTGAGCTCTTCTGGAACCCAGGGGTGCTGATC
CAGGCCGAGGACCGTGTGCACCGCATTGGACAGACAAACTCTGTGAGCATCCACTACCTGGTGGCAAAAG
GCACAGCAGATGACTACCTTTGGCCTCTGATTCAAGAGAAGATTAAAGTTCTGGGGGAAGCTGGGCTTTC
TGAGACCAATTTCTCGGAAATGACGGAAGCTACAGACTACGTGCACAAGGACCCAAAGCAGAAGACGATC
TATGACCTGTTCCAGCAGTCCTTTGAAGACGATGGAAATGACATGGAATTCCTGGAAGCAGCAGAGTCCT
TTGAGCTAGGAAGTACTTCAGGAACCTCTGGAAACATTTCCCAGGATCTGGGAGATCTCTTAGATGAAGA
CGAAGGCAGTCCGCCAAAGAAAAGCAGATTTGAATTCTTTGATAATTGGGACAGCTTTTCCTCTCCCTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211150 representing NM_018817
Red=Cloning site Green=Tags(s)

MSLPLTEEQRKKIEENRQKALARRAEKLSEQPQSAASGSSAAGPSQSKQGSLLNLLAEPSKPVGHASIFK
QQNLSNSFPTDQRPHSSRCSQPSPAEETTGLWKTQGEMSTACPKPNPSPPGASNQPLLGYKSSEGQPQAT
WDTGASSSGPFPRDPELEAKAARPSTSRQSISDSFYVLGGKTPRTEGRPPNILQTTPQNTGFLRGACIKT
GDRFRVKIGYNQELIAVFKSLPSRHYDSFTKTWDFSMSDYRALMKAVERLSTVSLKPLDEAGGSVGGQTS
LPSAPSLTFVTGKCMLISRVRFEVDIGYSEAVIGLFKQMESRSYDIKTRKWSFLLEEHNKLIARSRELKQ
VQLDPLPKTVTLAFASQLEKTSPKLKADVPEADLSGVDAKLVSSLMPFQREGVSFAISKRGRLLLADDMG
LGKTVQAICIAAFYRKEWPLLVVVPSSVRFTWEQAFLRWLPSLSPENINVVVTGKGRLTAGLVNIVSFDL
LCKLERQLKTPFKVVIIDESHFLKNIKTARCRAAVPILKVAKRVILLSGTPAMSRPAELYTQIIAVKPTF
FPQFHAFGLRYCDAKRLPWGWDYSGSSNLGELKLLLEEAIMLRRLKSDVLSQLPAKQRKMVVVNPGRISS
RAKAALDAAAKEMTKDKTKQQQKEALLVFFNRTAEAKIPCVVEYILDLLDSGREKFLVFAHHKVILDAVA
KELERKNVQHIRIDGSTPSADREAQCQRFQLSKGHTVALLSITAANMGLTFSTADLVVFAELFWNPGVLI
QAEDRVHRIGQTNSVSIHYLVAKGTADDYLWPLIQEKIKVLGEAGLSETNFSEMTEATDYVHKDPKQKTI
YDLFQQSFEDDGNDMEFLEAAESFELGSTSGTSGNISQDLGDLLDEDEGSPPKKSRFEFFDNWDSFSSPF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018817
ORF Size 2730 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018817.2, NP_061287.2
RefSeq Size 6715 bp
RefSeq ORF 2733 bp
Locus ID 54380
UniProt ID Q8BJL0
Cytogenetics 1 36.72 cM
MW 101.3 kDa
Summary ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_018817" in other vectors (4)
SKU Description Size Price
MC201448 Smarcal1 (untagged) - Mouse SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (Smarcal1), (10ug) 10 ug
$834.00
MG211150 Smarcal1 (tGFP-tagged) - Mouse Swi/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (Smarcal1) 10 ug
$1,033.00
MR211150L3 Lenti ORF clone of Smarcal1 (Myc-DDK-tagged) - Mouse SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (Smarcal1) 10 ug
$1,133.00
MR211150L4 Lenti ORF clone of Smarcal1 (mGFP-tagged) - Mouse SWI/SNF related matrix associated, actin dependent regulator of chromatin, subfamily a-like 1 (Smarcal1) 10 ug
$1,133.00

Plasmid Map for MR211150

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211150
Circular map for MR211150

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products