Ankrd24 (NM_027480) Mouse Tagged ORF Clone

CAT#: MR211137

Ankrd24 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 24 (Ankrd24)

Product Images

Circular map for MR211137

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankrd24
• Synonyms: 4631433D01Rik; 5730519E19Rik; D10Bur2e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211137 representing NM_027480
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACCCTCCGGGCACGATTCAAGAAAACAGAGGGCCAAGACTGGGGCAAAAGTGACCAGCGACTGC
  TCCAGGCAGTGGAGAACAACGATGTGGCCAGAGTGGCCTCGTTGATCGCCCACAAGGGGCTGGTGCCTAC
  AAAACTGGACCCCGAGGGCAAGTCTGCGTTCCACTTAGCAGCCATGAGGGGTGCGGCGGGGTGTCTGGAA
  GTGATGCTGGCTCAAGGTGCCGATGTCATGAGCACGGATGGAGCAGGTTACAATGCCCTTCACCTGGCAG
  CCAAGTACGGGCACCCAGAGTGTCTGAAGCAACTCTTGGAGGCTTCCTGCGTGGTGGACATCGAGGACAG
  CAGTGGGTGGACAGCCCTTCACCATGCAGCGGCTGGTGGTTGTCTGTCCTGCTCAAAGCTGCTCTGTTCC
  TTTAAGGCACACATGAACCCCCGGGACCGGTCGGGTGCCACACCCCTCATCATAGCGGCTCAGATGTGTC
  ACACAGATCTGTGCCGCCTCCTCCTACAGCAGGGGGCTGCCACAAATGACCAGGACCTGCAAGGCAGGAC
  AGCCTTGATGCTGGCATGTGAGGGGGGCAGCCCTGAGACTGTTGAAGTACTCCTGCAGGGTGGAGCCCAG
  TTGAGCATCACTGATGCACTGGGCCAGGATGCCACTCACTACGGAGCCCTGACTGGAGACAAACTCATCT
  TGCAGCTTCTACACGAGTCTGCACGGCGCTCTTCACCTCCCAGTGCCTCTCTAGAGGAGGACTCCGGAGA
  GGCCTCATCCCAGAACTCAGTGTCAAGCCATGAGAAACAAGGAGCCCCCAAGAAGAGGAAGGCACCCCAG
  CCTCCAGCCAGCACACCAGTTCCTGACGATCGGGATGCTTATGAGGAGATTGTACGCCTGAGACAAGAGA
  GAGGCCGGCTCCTGCAAAAGATCAGGGGCCTGGAACAGCACAAGGAACGGAGGAGGAAGGAGCCCCTGGA
  GGCGGAGGCCAGCTCAGTGCACAGCCTGGAGAGACAGGTGCAGGAGCTGCAACAGATGCTGGCGGAGAAG
  CAGGAGGAGAAGGAGAGCCTGGGCCGGGAGGTGGAGAGCCTGCAGAGCCGTCTGTCCCTGCTGGAGAATG
  AGAGAGAAAACACAAGCTATGATGTAGCTACCCTGCAGGATGAGGAGGGTGAGATGCCTGACTTCCCAGG
  AGCTGATGCACTGATGCCAAAGAACCAGAGTCCATCAGCAGAGGAAATAGTTGCTTCCCTACAGGAGCAG
  GTGGCTCAGCTCACCAGGCAGAACCAGGAGCTGCTGGAAAAGGTCCAGATCCTTGAGGAGTTTGAAAAGG
  ATGAGGCGCAGATGGCAGAAGAAAGTCAGGCCGAGGTCGTCCCCCTGGTCTTGTATGAATCCTTACGGGC
  AGAGCTTGAGCAGCTTCGGAGGCAATACACAGAGGCCATGCATTCGCAGCAGCAGCAGCAGGAGGGGGAG
  CCACCCAGGGCTCAAGAAGGAGAAGAGACAGCATACCAGGAAATCAAGGATAAGGGAATCACTATCCAGA
  ATGGACCCAGCGTCCCAGACCTCAACGGCACCACATATGCAGAAACCAAAGCAAATGGAATGGAACTCCA
  AGCAGGAGGCTCCAAGGGTGTCTGGAATACTGAAGCAGGGGTTTCAGAAGCAGCACCCATAGAACCCGAG
  GCTGCAGGTTCAGAGGCCACGGGGAAAGACAGACTGGCAGCCAAGGAAATGGACACTAGTGCTACTATGG
  CTGAGGCCCTAAATGTGAAAGCTCTAGGTGACAATGCCGAAAGTGAGCCAGTGGCTGCCGAAGATACAGG
  AGGAAAAGAGAATCCAGGAATGAAAGCTGATGAGGTGGATGTGTTGGCACAGGCAGGGCTCACAGGTACA
  GTGATTAGAAACATGGAGGCCATCGGAGTGCGGGATACAGGAATTCAGGCCACAGGATTAGAGGCTAAGG
  CAGTGAAGACCACTGGAGTGCAGGCCACAGTGGCGGAGGTCATAGGAGTGAAGGTCACAGGAGTGCAGAC
  CACAGCGATAGAAGCTATAGGAGTCAAGGACACCACCCAGGTGGCCACAGGAGCGCAGGCCGATTGCTGG
  CAGGCCACGGAGGCAGACTGCACCGGAGCGCAGGACACAGCTATGGAGCCCACGGGGGCACAGGCCACTG
  TGACAGAGACTACGGAAGCCGAGACCAGCGGCACGGAGGACCCTTGCGCTGCGATCCTGCACCCGGGGGC
  GGCGGCGGCGGCGCTGCAGGCCGAGCTGGAGACCCGGATCCGCGGTCTGGAGGAGGCGCTACGCAGAAGG
  GAGCGGGAGGCTGCGGCCGAGTTAGAGGCTGCTCGGGGCCGTTTTGCAGAGGCTGAGGAGGCGGCGCGGG
  GGCGGAGCCGCGAGCTAGAGGCGCTTCGGGAATTGCTGGCCACGGCCACGGCTACCGGCGAGCGCGCACG
  CACAGAGGCCGCCGAGTTGCGCCAAGCGCTAGCTGCCTCCGAGGCTCGGGTCGCCGAACTCAGCTCTACC
  GTGGACGCCGCTCGCGAGGAGCTGGAGCGCATGCGCGGGGCCTCGGTTCCTGCGGACGAGCACGAGCACG
  CGCTGAGCGCCCTGCGCGACCACGTGACCCGGCTGCAGGCGCAGCTGGCGGACCTGGCGCGCAGGCACGA
  GAAGACTAGCGCAGAGGTCTTCCAGATCACAGACCTGTCCAAAGAAGTCTTTACACTGAAGGAGGCTCTG
  AAGGTGCAGCAGTCCACCCCAGCCAGCTCCAAGGAGGAGGAGGCTCTGCGCGGCCAGGTGACAGCCCTGC
  AGCAGCAGATACAGGAGGAGGCCCGGGAGCATGGCGCTGTGGTGGCTTTATACCGCACCCATCTCCTGTA
  TGCCATTCAGGGACAGATGGATGAGGATGTACAGTGCATTTTGAGCCAGATTCTGCAGATGCAGCGGCTT
  CAGGCTCAGGGTCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211137 representing NM_027480
  Red=Cloning site Green=Tags(s)
  
  MKTLRARFKKTEGQDWGKSDQRLLQAVENNDVARVASLIAHKGLVPTKLDPEGKSAFHLAAMRGAAGCLE
  VMLAQGADVMSTDGAGYNALHLAAKYGHPECLKQLLEASCVVDIEDSSGWTALHHAAAGGCLSCSKLLCS
  FKAHMNPRDRSGATPLIIAAQMCHTDLCRLLLQQGAATNDQDLQGRTALMLACEGGSPETVEVLLQGGAQ
  LSITDALGQDATHYGALTGDKLILQLLHESARRSSPPSASLEEDSGEASSQNSVSSHEKQGAPKKRKAPQ
  PPASTPVPDDRDAYEEIVRLRQERGRLLQKIRGLEQHKERRRKEPLEAEASSVHSLERQVQELQQMLAEK
  QEEKESLGREVESLQSRLSLLENERENTSYDVATLQDEEGEMPDFPGADALMPKNQSPSAEEIVASLQEQ
  VAQLTRQNQELLEKVQILEEFEKDEAQMAEESQAEVVPLVLYESLRAELEQLRRQYTEAMHSQQQQQEGE
  PPRAQEGEETAYQEIKDKGITIQNGPSVPDLNGTTYAETKANGMELQAGGSKGVWNTEAGVSEAAPIEPE
  AAGSEATGKDRLAAKEMDTSATMAEALNVKALGDNAESEPVAAEDTGGKENPGMKADEVDVLAQAGLTGT
  VIRNMEAIGVRDTGIQATGLEAKAVKTTGVQATVAEVIGVKVTGVQTTAIEAIGVKDTTQVATGAQADCW
  QATEADCTGAQDTAMEPTGAQATVTETTEAETSGTEDPCAAILHPGAAAAALQAELETRIRGLEEALRRR
  EREAAAELEAARGRFAEAEEAARGRSRELEALRELLATATATGERARTEAAELRQALAASEARVAELSST
  VDAAREELERMRGASVPADEHEHALSALRDHVTRLQAQLADLARRHEKTSAEVFQITDLSKEVFTLKEAL
  KVQQSTPASSKEEEALRGQVTALQQQIQEEAREHGAVVALYRTHLLYAIQGQMDEDVQCILSQILQMQRL
  QAQGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_027480
• ORF Size: 2955 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027480.3, NP_081756.4
• RefSeq Size: 3658 bp
• RefSeq ORF: 2958 bp
• Locus ID: 70615
• UniProt ID: Q80VM7
• Cytogenetics: 10 39.72 cM
• MW: 106.2 kDa