Zfp748 (NM_001035231) Mouse Tagged ORF Clone

CAT#: MR211071

Zfp748 (Myc-DDK-tagged) - Mouse zinc finger protein 748 (Zfp748), transcript variant 1

Product Images

Circular map for MR211071

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zfp748
• Synonyms: KRAB-O; mszf54; Rslcan20/21; Zfp208
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211071 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAAATGCTGTCATTCAGGGATGTGGCCGTTGATTTCTCTGCAGAGGAGTGGGAGTGCCTGGAGC
  CTGCTCAGTGGAATCTGTACAGGGACGTGATGCTGGAGAATTACAGCCACCTTGTGTTCCTGGGTCTTGC
  TTTCTCTAAGCCATACCTGGTCACGTTTCTGGAGCAAAGACAAGAGCCTTTGAATGAGAAGAGACAAGTG
  GCCACCACCATGCACCCAGATATAAAACCATATAAATGTAAAGAATGTGGCAAAGCCTGTGATTGGAACT
  CACTCCTTCTCGAACACCAGAGAACCAATCCAGGAGAGAATGCCTACAAGTGTGAAGAATGTGGTCAGGC
  TTCTGGCTCTTACTCAGTAATTCCTGAACACCATATAAACGATACGGAAGAGAAAGCCTGCAAGTGTGAA
  GAATGTGGTAAAGTCATTTGTACTTGCTCAGAAAATTCTAGCTACCAGAGAATTTGCATTGGAGAAAACC
  CCTACAAGTGTGAAGAATGTGGAAAAGCCTTTTCTTCTCATTCATGTCTTGCTCAACATGAGGTAGAACA
  CACTGGACAGCAATTTTATAACTGTGAAGAATGTGGGAAACTGTTTTATTGTCCTTCTCACCTTACAGAA
  CATCAAAAAATTCATTCTCAAGAGAATCTCTTTAAGATTGAGGTATGTAGCGAGGTCTTTTGTGCTCCCA
  TAGAACTTTCTAAAGACCAGAACTTTTGTACTGAAGAAAAACCCTACAGGTATGAAGACTATGTTAAGGC
  CTTTAGCGCTTGCTCAGTTCTTTCTGAACACCCGACAATTCATCCAGGAGAGAAAGCATTCAAGTGTGAA
  GAATGTGGCAATGCCTTTTGTACACTCCATTCAGTCTCTAAAATGAAAATTCATTATGAAGTGAAATCCT
  ACAAGTGTGAAGAGTGTGGGAAAGCCTTTGCTACTCATCTTTCCCTGATTCAGCATAAGATAGGTCATAC
  TCGAGAGAAACCTTATCAATGTGAAGAATGTGGCAAAATGTTTTACTGTTCTTCAAACCTTAAGCAACAT
  CAAATAACTCATTCTCAAGAGAAACCCTACAAGTGTGAAGTATGTGGCAAGGTCTTTAGAACTTGCTGGC
  AACTTTCTAAACACCTGAGAATCCATTCTGGAGAGAAACCATACAAGTGTGAGGAATGTGGCAAAGCCTT
  TTATACTCTCTCCTACCTTACTCAGCACAAATTAGGTCACACTGGGGAGAAACCTTACAAATGTGAGGAA
  TGTGGCAAAACCTTTTACTACCCTTCAGTCCTTAAGGAACACCTAGCAATTCATTCTGGAGAGAAACCGT
  ACAGGTGTGATGAATGTGGCAAGGACTTTTGTACTCGCTCAGGACGTTCCAGACACCAGAGAATCCATAC
  TGGAGAGAAACCGTACAAGTGTGAGCAATGTGGAAAGGCCTTCAGTACCCATTCATACCTTTCTCATCAC
  AAGATAGTTCACACTGGACATAAACCCTACAAGTGTGAAGAGTGTGGCAAAAAATTTTATTATCCTTCTC
  GACTTAAGGAGCATCAAAGGATTCATTCTCAAGAGAATCCCTACAAGTGTGAAATATGTGGCAAAGCCTT
  TTATACTCACTCATACTTTACCCAGCACAAATTAGGTCACACTGGGGAGAAACCTTACAAATGTGAGGAA
  TGTGGCAAAACCTTTTACTACCCTTCAATCCTTAAGGAACACCTAGCAATTCATTCTGGAAAGAAACCGT
  ACAGGTGTGAAGAATGTGGCAAGGACTTTTGTACTCGCTCAGGACGTTCCAGACACCAAAGAATCCATAC
  TGGAGAGAAACCCCACAAGTGTGAAGAATGCGGAAAAGTCTTTTCTACTCATTCATATCTTACTCAGCAC
  AAAGTAGTCCACTCTGGAGAGAAGCCCTACAAGTGTGAGGAGTGTGGCAAAAAGTTTTACTATCCTTCTC
  GCCTTAAGGAGCATCAAAGGATTCATTCTCAAGAGAATCCTTACAAGTGTGAAATATGTGGCAATGTATT
  TTGTACTCCCAAAGGACTTTCGAAACACCAGAGATTTCATACTGGAGAGAAACCCTACAAGTGTGAAGAA
  TGCGGGAAAATGTTTTATTATCCTTCTCGACTTAAGGAACATCAAAGAATTCATTCTCAAGAGAATCCTT
  ACAAGTGTGAAATATGTGGCAAAGCCTTTTATACTCACTCCTACCTTACTCAGCACAAATTAGGTCATAC
  TGGGGAGAAACCTTACAAATGTGAGGAATGTGGCAAAACCTTTTACTACCCTTCAATCCTTAAGGAACAC
  CTAGCAATTCATTCTGGAAAGAAACCGTACAGGTGTGAAGAATGTGGCAAGGACTTTTGTACTCGCTCAG
  GACGTTCCAGACACCAGAGAATCCATACTGGAGAGAAACCCTACAAGTGTGAGCAATGTGGAAAGGCCTT
  CAGTACTCACTCATACCTTTCTCAACACAAGGTAGTCCACTCTGGAGAGAAGCCCTACAAGTGTGAAGAG
  TGTGGCAAAATGTTTTACTATTCTTCTCGACTTAAGGAGCATCAAAGGATTCATTCTCAAGAGAATCCCT
  ACAAGTGTGAAGTGTGTGGCAAAGTCTTTAGTGCTCACTTAGAACTTGCTACCCACCTAAGTATTCATTC
  CGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211071 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEMLSFRDVAVDFSAEEWECLEPAQWNLYRDVMLENYSHLVFLGLAFSKPYLVTFLEQRQEPLNEKRQV
  ATTMHPDIKPYKCKECGKACDWNSLLLEHQRTNPGENAYKCEECGQASGSYSVIPEHHINDTEEKACKCE
  ECGKVICTCSENSSYQRICIGENPYKCEECGKAFSSHSCLAQHEVEHTGQQFYNCEECGKLFYCPSHLTE
  HQKIHSQENLFKIEVCSEVFCAPIELSKDQNFCTEEKPYRYEDYVKAFSACSVLSEHPTIHPGEKAFKCE
  ECGNAFCTLHSVSKMKIHYEVKSYKCEECGKAFATHLSLIQHKIGHTREKPYQCEECGKMFYCSSNLKQH
  QITHSQEKPYKCEVCGKVFRTCWQLSKHLRIHSGEKPYKCEECGKAFYTLSYLTQHKLGHTGEKPYKCEE
  CGKTFYYPSVLKEHLAIHSGEKPYRCDECGKDFCTRSGRSRHQRIHTGEKPYKCEQCGKAFSTHSYLSHH
  KIVHTGHKPYKCEECGKKFYYPSRLKEHQRIHSQENPYKCEICGKAFYTHSYFTQHKLGHTGEKPYKCEE
  CGKTFYYPSILKEHLAIHSGKKPYRCEECGKDFCTRSGRSRHQRIHTGEKPHKCEECGKVFSTHSYLTQH
  KVVHSGEKPYKCEECGKKFYYPSRLKEHQRIHSQENPYKCEICGNVFCTPKGLSKHQRFHTGEKPYKCEE
  CGKMFYYPSRLKEHQRIHSQENPYKCEICGKAFYTHSYLTQHKLGHTGEKPYKCEECGKTFYYPSILKEH
  LAIHSGKKPYRCEECGKDFCTRSGRSRHQRIHTGEKPYKCEQCGKAFSTHSYLSQHKVVHSGEKPYKCEE
  CGKMFYYSSRLKEHQRIHSQENPYKCEVCGKVFSAHLELATHLSIHSG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001035231
• ORF Size: 2667 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001035231.1
• RefSeq Size: 4615 bp
• RefSeq ORF: 2667 bp
• Locus ID: 212276
• Cytogenetics: 13 B3
• MW: 103.5 kDa