Map3k20 (NM_023057) Mouse Tagged ORF Clone

CAT#: MR210730

B230120H23Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA B230120H23 gene (B230120H23Rik), transcript variant 1

Product Images

Circular map for MR210730

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Map3k20
• Synonyms: AV006891; B230120H23Rik; MLTK; MLTKalpha; MLTKbeta; Zak
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210730 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCTCTCGGAGCCTCCTTTGTGCAAATTAAGTTCGACGACTTGCAGTTTTTTGAAAACTGCGGTG
  GAGGAAGTTTTGGGAGTGTGTATCGAGCCAAATGGATATCACAGGACAAGGAGGTGGCTGTAAAGAAGTT
  ACTCAAAATAGAGAAAGAGGCAGAAATCCTGAGCGTCCTCAGTCACAGAAACATCATCCAGTTTTATGGA
  GTGATTCTGGAACCTCCCAACTATGGCATCGTCACAGAATATGCTTCCCTGGGCTCCCTGTATGATTACA
  TTAACAGCAACAGGAGTGAAGAGATGGACATGGAACACATCATGACCTGGGCCACTGACGTAGCCAAAGG
  GATGCATTACTTACACATGGAAGCTCCTGTCAAGGTGATCCACAGAGACCTCAAGTCAAGAAACGTTGTT
  ATCGCTGCTGACGGAGTGCTGAAGATCTGTGACTTCGGTGCCTCGCGGTTCCATAACCACACAACACACA
  TGTCCTTGGTTGGAACTTTCCCATGGATGGCTCCAGAAGTTATCCAGAGTCTCCCTGTGTCTGAAACCTG
  TGACACGTATTCCTATGGTGTGGTTCTCTGGGAGATGCTAACAAGGGAGGTCCCCTTTAAAGGTTTGGAA
  GGATTACAAGTAGCTTGGCTTGTAGTGGAAAAAAACGAGAGATTAACCATTCCAAGCAGCTGCCCCAGAA
  GCTTTGCTGAACTGCTACACCAGTGTTGGGAGGCTGATGCCAAGAAACGGCCATCGTTCAAGCAAATCAT
  TTCAATCCTGGAGTCTATGTCAAATGACACGAACCTTCCTGACCAGTGTAACTCATTTTTACACAACAAG
  GCGGAGTGGAGGTGTGAAATTGAGGCAACCCTTGAGCGACTGAAGAAACTAGAGCGAGATCTCAGCTTTA
  AAGAGCAGGAGCTCAAAGAACGGGAGAGACGTCTCAAGATGTGGGAGCAGAAGCTGACGGAGCAATCCAA
  CACCCCGCTGCTGCCTTCCTTTGAGATTGGTGCGTGGACTGAAGACGATGTGTATTTTTGGGTTCAGCAG
  CTCGTCAGAAAAGGCGAGTCTTCAGTAGAGATGAGTGGATACGCAAGTTTGTTTAAGGAGAACAACATCA
  CAGGCAAGCGGTTGCTGCTTCTGGAAGAGGAAGATCTGAAAGACATGGGCATCGTCTCCAAGGGACACAT
  CATTCACTTTAAGTCAGCTATTGAGAAGCTAACCCACGATTATCTGAACCTGTTTCACTTCCCACCACTG
  ATTAAGGATTCAGGAGGGGAACCTGAAGAAAATGAGGAAAAAATAGTGAACCTTGAACTTGTTTTTGGTT
  TTCACTTGAAGCCAGGAACTGGCCCACAGGATTGCAAATGGAAAATGTATATGGAGATGGATGGGGACGA
  AGTTGCAATCACGTACATAAAAGATGTGACTTTCAACACGAGCCTCCCTGATGCAGAGATTTTAAAGATG
  ACAAAGCCACCATTTGTAATGGAGAAGTGGATCGTGGGAATAGCGGAGGATCAGACTGTGGAGTGCACGG
  TCACCTATGAGAATGATGTCAGAACACCAAAACTCACTAAGCACGTGCACTCCATACAGTGGGACAGAAC
  GAAGCCTCAGGATGAGGTGAAGGCGGTCCAGCTTGCCATTCAGACTCTGTTCTCCAGCTCAGAGGGCAAC
  CCTGGCAGCAGATCCGACTCCAGTGCGGACTGCCAATGGTTAGACACTCTGCGCATGCGGCAGATTGCAT
  CCCACACTTCTCTACAGCGTTCCCAAAGCAACCCTATCTTGGGGTCTCCTTTCTTCCCTTACTTTGCCAA
  CCAGGATTCCTATGCTGCTGCGGTGAGGAGGACCCAGACTCCGGTGAAGTACCAGCAGATTACACCAAGC
  ATCAACCCTTCCCGGAGCTCCTCCCCCACGCAGTATGGGCTGTCCAGAAACTTCTCCTCCCTGAACCTCA
  GCTCCAGGGACAGCGGCTTCTCCAGCCTCAATGACAGCTCCTCCGAGAGGGGCCGCTACTCAGACCGAAG
  CAGAAACAAGTACTACCGTGGTAGTGTGTCCCTCAATTCTTCCCCGAAAGGAAGATATGGTGGGAAAAGT
  CAGCATTCCACACCTTCAAGAGAAAGATATTCTGGAAAGTTCTACAGGCTGCCCCAGTCAGCGCTGAACA
  CTCATCAGTCCCCTGACTTCAAGAGGAGCCCAAATGACCATGACCGCCGTGTGCCCAGGACCATACCCGG
  GATGCCTCTGCACCCTGAGACTGCCTCCAAAGCAGGGGAAGAGGAGAGCAGGGTGAGCGAAGGGGGCTGG
  ACTAAAGTGGAATATCGGAAGAAGACACACAGGCAACTTTCGGCCAAGACTAGCAAGGAGAGAACCCGTG
  GCAACTACCGTGGGCGGCGGAATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210730 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYG
  VILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMEHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVV
  IAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLE
  GLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTNLPDQCNSFLHNK
  AEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYFWVQQ
  LVRKGESSVEMSGYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYLNLFHFPPL
  IKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEVAITYIKDVTFNTSLPDAEILKM
  TKPPFVMEKWIVGIAEDQTVECTVTYENDVRTPKLTKHVHSIQWDRTKPQDEVKAVQLAIQTLFSSSEGN
  PGSRSDSSADCQWLDTLRMRQIASHTSLQRSQSNPILGSPFFPYFANQDSYAAAVRRTQTPVKYQQITPS
  INPSRSSSPTQYGLSRNFSSLNLSSRDSGFSSLNDSSSERGRYSDRSRNKYYRGSVSLNSSPKGRYGGKS
  QHSTPSRERYSGKFYRLPQSALNTHQSPDFKRSPNDHDRRVPRTIPGMPLHPETASKAGEEESRVSEGGW
  TKVEYRKKTHRQLSAKTSKERTRGNYRGRRNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023057
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023057.5, NP_075544.1
• RefSeq Size: 3365 bp
• RefSeq ORF: 2409 bp
• Locus ID: 65964
• UniProt ID: Q9ESL4
• Cytogenetics: 2 C3
• MW: 91.7 kDa
• Gene Summary: Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways (PubMed:11042189). Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14 (By similarity). Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2 (By similarity). Involved in limb development (PubMed:26755636).[UniProtKB/Swiss-Prot Function]