Furin (NM_001081454) Mouse Tagged ORF Clone

CAT#: MR210693

Furin (Myc-DDK-tagged) - Mouse furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 2

Product Images

Circular map for MR210693

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Furin
• Synonyms: 9130404I01Rik; Fu; Fur; PA; PACE; Pcs; Pcsk3; SP; SPC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210693 representing NM_001081454
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGAGATCCTGGTTGCTATGGGTGGTCGCAGCAGCAGGAGCCGTGGTCCTGCTGGCAGCTGATG
  CTCAAGGCCAGAAGATCTTCACCAACACCTGGGCCGTGCACATTCCTGGAGGCCCAGCTGTGGCTGATAG
  GGTGGCGCAGAAGCATGGCTTCCACAACCTGGGCCAGATCTTCGGTGACTATTACCACTTCTGGCACAGA
  GCAGTGACAAAGCGGTCCCTGTCGCCTCACCGCCCGCGGCACAGCCGGCTACAGAGGGAGCCTCAAGTAA
  AGTGGCTGGAGCAGCAGGTAGCCAAGCGAAGAGCCAAGAGGGACGTGTATCAGGAGCCCACGGACCCCAA
  GTTCCCCCAGCAGTGGTACCTGTCTGGTGTCACTCAGCGAGACCTGAATGTGAAGGAGGCCTGGGCCCAG
  GGCTTCACAGGCCATGGCATTGTGGTCTCCATCCTGGATGACGGCATTGAGAAGAATCATCCCGACCTAG
  CAGGCAATTATGACCCTGGAGCCAGTTTTGACGTGAATGACCAGGACCCCGACCCACAGCCTCGGTACAC
  ACAGATGAATGACAACAGGCATGGCACTCGCTGTGCCGGGGAAGTGGCAGCAGTGGCCAACAATGGTGTC
  TGTGGCGTAGGTGTAGCTTACAATGCCCGAATTGGAGGGGTGCGGATGTTGGATGGCGAGGTGACTGATG
  CAGTAGAGGCACGTTCGCTGGGCCTGAATCCCAACCACATCCACATCTACAGCGCCAGCTGGGGCCCTGA
  GGACGACGGCAAGACCGTGGATGGACCAGCCCGGCTCGCTGAGGAGGCCTTCTTTCGGGGAGTTAGCCAG
  GGCCGAGGAGGGCTGGGCTCCATCTTTGTCTGGGCCTCAGGGAATGGGGGCCGGGAACATGACAGCTGCA
  ACTGTGACGGCTACACCAACAGCATCTATACACTGTCCATCAGCAGCGCCACACAGTTCGGCAATGTGCC
  CTGGTACAGTGAGGCCTGCTCCTCCACACTGGCCACCACCTACAGCAGCGGCAACCAGAATGAGAAGCAG
  ATCGTGACAACTGACCTGAGGCAGAAGTGCACAGAATCTCACACAGGCACCTCGGCTTCTGCCCCCTTGG
  CAGCTGGTATCATTGCTCTCACCCTGGAGGCCAACAAGAACCTCACCTGGCGGGACATGCAGCACCTGGT
  AGTGCAGACCTCAAAGCCAGCCCACCTCAACGCTGATGATTGGGCTACCAACGGCGTGGGCCGGAAAGTG
  AGCCATTCGTATGGCTACGGGCTGTTGGATGCAGGTGCCATGGTGGCTCTGGCCCAGAACTGGACAACAG
  TGGCCCCCCAGCGGAAGTGCATTGTTGAAATCCTGGTGGAACCCAAGGACATCGGCAAACGGCTAGAGGT
  GCGCAAGGCGGTGACAGCATGCCTGGGTGAGCCCAACCACATCACCAGGCTGGAACACGTTCAGGCCCGG
  CTCACCCTGTCTTACAATCGCCGCGGTGACCTGGCTATCCACCTCATCAGCCCCATGGGCACCCGCTCCA
  CCCTGTTAGCTGCCAGACCACATGACTACTCTGCTGATGGGTTTAATGATTGGGCTTTCATGACAACCCA
  TTCCTGGGATGAGGATCCGGCTGGCGAATGGGTCCTAGAGATTGAAAATACCAGTGAAGCCAACAACTAT
  GGGACGCTGACCAAGTTCACTCTCGTTCTGTATGGCACAGCCCCCGAGGGGCTCTCTACACCTCCAGAAA
  GCAGCGGCTGCAAGACCCTCACATCCAGCCAGGCCTGCGTGGTGTGTGAGGAAGGTTACTCTCTGCACCA
  GAAAAGCTGTGTCCAGCACTGCCCACCAGGCTTCATCCCCCAAGTCCTTGATACACACTACAGCACTGAG
  AATGATGTGGAGATCATCCGTGCCAGCGTCTGTACCCCCTGCCACGCCTCATGTGCCACTTGCCAGGGCC
  CGGCCCCCACAGACTGCCTCAGCTGCCCCAGCCATGCCTCCCTGGACCCAGTGGAGCAGACCTGCTCCCG
  GCAAAGCCAGAGCAGCCGGGAGTCTCGGCCTCAGCAGCAGCCTCCTGCACTGCGCCCTGAGGTGGAGATG
  GAGCCCCGGCTGCAGGCTGGGTTGGCCTCTCACCTGCCCGAGGTCCTGGCCGGCCTCAGCTGCCTTATCA
  TCGTGCTCATCTTTGGCATCGTCTTCCTGTTCCTGCATCGGTGTTCGGGCTTCAGCTTCCGGGGAGTGAA
  AGTGTACACCATGGACCGCGGCCTCATCTCCTACAAGGGGCTGCCCCCTGAAGCCTGGCAGGAGGAGTGC
  CCATCGGACTCAGAGGAGGACGAGGGCCGGGGCGAGAGGACCGCCTTCATCAAAGACCAGAGCGCCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210693 representing NM_001081454
  Red=Cloning site Green=Tags(s)
  
  MELRSWLLWVVAAAGAVVLLAADAQGQKIFTNTWAVHIPGGPAVADRVAQKHGFHNLGQIFGDYYHFWHR
  AVTKRSLSPHRPRHSRLQREPQVKWLEQQVAKRRAKRDVYQEPTDPKFPQQWYLSGVTQRDLNVKEAWAQ
  GFTGHGIVVSILDDGIEKNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGV
  CGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLAEEAFFRGVSQ
  GRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQ
  IVTTDLRQKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLNADDWATNGVGRKV
  SHSYGYGLLDAGAMVALAQNWTTVAPQRKCIVEILVEPKDIGKRLEVRKAVTACLGEPNHITRLEHVQAR
  LTLSYNRRGDLAIHLISPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPAGEWVLEIENTSEANNY
  GTLTKFTLVLYGTAPEGLSTPPESSGCKTLTSSQACVVCEEGYSLHQKSCVQHCPPGFIPQVLDTHYSTE
  NDVEIIRASVCTPCHASCATCQGPAPTDCLSCPSHASLDPVEQTCSRQSQSSRESRPQQQPPALRPEVEM
  EPRLQAGLASHLPEVLAGLSCLIIVLIFGIVFLFLHRCSGFSFRGVKVYTMDRGLISYKGLPPEAWQEEC
  PSDSEEDEGRGERTAFIKDQSAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081454
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081454.2
• RefSeq Size: 4166 bp
• RefSeq ORF: 2382 bp
• Locus ID: 18550
• UniProt ID: P23188
• Cytogenetics: 7 45.65 cM
• MW: 87.2 kDa
• Gene Summary: This gene encodes a calcium-dependent serine endoprotease that proteolytically activates different proprotein substrates traversing the secretory pathway. The encoded protein undergoes proteolytic autoactivation during which an N-terminal propeptide is cleaved to generate the mature protein. Mice lacking the encoded protein die at an embryonic stage and display hemodynamic insufficiency, cardiac ventral closure defect, axial rotation defect and abnormal yolk sac vasculature. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]