Stat5a (NM_011488) Mouse Tagged ORF Clone

CAT#: MR210689

Stat5a (Myc-DDK-tagged) - Mouse signal transducer and activator of transcription 5A (Stat5a), transcript variant 1

Product Images

Circular map for MR210689

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Stat5a
• Synonyms: AA959963; STAT5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210689 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCTGGATTCAGGCCCAGCAGCTTCAGGGAGATGCCCTGCGCCAGATGCAAGTGTTGTATGGGC
  AGCATTTCCCCATCGAGGTCCGGCACTACCTGGCCCAGTGGATCGAGAGCCAGCCGTGGGATGCTATTGA
  CTTGGATAATCCCCAGGACCGAGGTCAGGCCACCCAACTCCTGGAGGGCCTGGTGCAGGAGCTGCAGAAG
  AAGGCGGAGCACCAGGTGGGGGAAGATGGGTTTTTGCTGAAGATCAAGCTGGGGCACTATGCCACACAGC
  TCCAGAACACGTATGACCGCTGTCCCATGGAGCTGGTTCGCTGTATCCGTCACATTCTGTACAACGAACA
  GAGGCTGGTTCGCGAAGCCAACAATTGCAGCTCCCCTGCTGGTGTCCTGGTTGACGCCATGTCCCAGAAG
  CACCTTCAGATCAACCAAAGGTTTGAGGAGCTGCGCCTGATCACACAGGACACGGAGAACGAGCTGAAGA
  AGCTGCAGCAGACCCAAGAGTACTTCATCATCCAGTACCAGGAGAGCCTGCGGATCCAAGCTCAGTTTGC
  CCAGCTGGGCCAGCTGAACCCCCAGGAGCGCATGAGCAGGGAGACGGCCCTCCAGCAGAAGCAAGTGTCC
  CTGGAGACCTGGCTGCAGCGAGAGGCACAGACACTGCAGCAGTACCGAGTGGAGCTGGCTGAGAAGCACC
  AGAAGACCCTGCAGCTGCTGCGGAAGCAGCAGACCATCATCCTGGACGACGAGCTGATCCAGTGGAAGCG
  GAGACAGCAGCTGGCCGGGAACGGGGGTCCCCCCGAGGGCAGCCTGGACGTGCTGCAGTCCTGGTGTGAG
  AAGCTGGCCGAGATCATCTGGCAGAACCGGCAGCAGATCCGCAGGGCTGAGCACCTGTGCCAGCAGCTGC
  CCATCCCAGGCCCCGTGGAGGAGATGCTGGCTGAGGTCAACGCCACCATCACGGACATCATCTCAGCTCT
  GGTCACCAGCACGTTCATCATCGAGAAGCAGCCTCCTCAGGTCCTGAAGACCCAGACCAAGTTTGCGGCC
  ACCGTGCGCCTGCTGGTGGGGGGAAAGCTGAATGTGCACATGAACCCCCCGCAGGTGAAGGCGACCATCA
  TCAGCGAGCAGCAGGCCAAGTCCCTGCTCAAGAATGAGAACACCCGCAATGAGTGCAGCGGCGAGATCCT
  GAACAACTGTTGCGTCATGGAGTACCACCAGGCCACTGGCACGCTCAGCGCCCACTTCAGAAACATGTCA
  CTGAAAAGAATCAAGCGCGCCGACAGGCGTGGTGCAGAGTCGGTGACGGAGGAGAAGTTCACAGTCCTGT
  TTGAGTCTCAGTTCAGCGTTGGCAGCAACGAGCTGGTGTTCCAGGTGAAGACCCTGTCCCTCCCTGTGGT
  CGTTATCGTCCATGGCAGCCAGGACCACAATGCTACTGCCACCGTGCTGTGGGACAATGCCTTTGCTGAG
  CCGGGCAGGGTGCCATTTGCTGTGCCTGACAAGGTGCTGTGGCCGCAGCTGTGTGAAGCGCTCAACATGA
  AATTCAAGGCTGAAGTACAGAGCAACCGGGGCTTGACCAAAGAGAACCTCGTGTTCCTGGCACAGAAACT
  GTTCAACATCAGCAGCAACCACCTCGAGGACTACAACAGCATGTCTGTGTCCTGGTCCCAGTTCAACCGG
  GAGAACTTGCCCGGCTGGAACTACACCTTCTGGCAGTGGTTCGACGGGGTGATGGAGGTGCTGAAGAAGC
  ACCATAAGCCCCATTGGAATGATGGGGCTATCCTGGGTTTCGTGAACAAGCAACAGGCCCACGACCTGCT
  CATCAACAAGCCGGACGGGACCTTCCTGCTGCGCTTCAGTGACTCGGAAATCGGGGGCATCACCATTGCT
  TGGAAGTTTGACTCTCCGGACCGAAACCTCTGGAATCTGAAGCCATTCACGACGCGAGATTTCTCCATTC
  GGTCCCTGGCCGACCGGCTGGGGGACCTGAACTACCTTATCTACGTGTTCCCAGACCGACCCAAGGACGA
  GGTCTTTGCCAAGTATTACACTCCTGTACTTGCGAAAGCAGTTGACGGATACGTGAAGCCACAGATCAAG
  CAAGTGGTCCCTGAGTTCGTCAATGCATCCACAGATGCCGGAGCCAGCGCCACCTACATGGACCAGGCTC
  CTTCCCCAGTCGTGTGCCCTCAACCTCACTACAACATGTACCCACCCAACCCTGACCCTGTCCTTGACCA
  AGATGGCGAGTTTGACCTGGATGAGAGCATGGATGTTGCCAGGCACGTGGAAGAACTTTTACGCCGGCCC
  ATGGACAGTCTCGACGCCCGCCTCTCCCCACCTGCTGGTCTCTTCACCTCCGCTAGAAGCTCCCTGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210689 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGWIQAQQLQGDALRQMQVLYGQHFPIEVRHYLAQWIESQPWDAIDLDNPQDRGQATQLLEGLVQELQK
  KAEHQVGEDGFLLKIKLGHYATQLQNTYDRCPMELVRCIRHILYNEQRLVREANNCSSPAGVLVDAMSQK
  HLQINQRFEELRLITQDTENELKKLQQTQEYFIIQYQESLRIQAQFAQLGQLNPQERMSRETALQQKQVS
  LETWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCE
  KLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAA
  TVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNECSGEILNNCCVMEYHQATGTLSAHFRNMS
  LKRIKRADRRGAESVTEEKFTVLFESQFSVGSNELVFQVKTLSLPVVVIVHGSQDHNATATVLWDNAFAE
  PGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNISSNHLEDYNSMSVSWSQFNR
  ENLPGWNYTFWQWFDGVMEVLKKHHKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIA
  WKFDSPDRNLWNLKPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVFAKYYTPVLAKAVDGYVKPQIK
  QVVPEFVNASTDAGASATYMDQAPSPVVCPQPHYNMYPPNPDPVLDQDGEFDLDESMDVARHVEELLRRP
  MDSLDARLSPPAGLFTSARSSLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011488
• ORF Size: 2382 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011488.3, NP_035618.1
• RefSeq Size: 3910 bp
• RefSeq ORF: 2382 bp
• Locus ID: 20850
• UniProt ID: P42230
• Cytogenetics: 11 63.77 cM
• MW: 90.8 kDa
• Gene Summary: Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.[UniProtKB/Swiss-Prot Function]