Eomes (NM_010136) Mouse Tagged ORF Clone

CAT#: MR210179

Eomes (Myc-DDK-tagged) - Mouse eomesodermin homolog (Xenopus laevis) (Eomes), transcript variant 1

Specifications

Product Data

• Product Name: Eomes (NM_010136) Mouse Tagged ORF Clone
• Symbol: Eomes
• Synonyms: C77258; TBR-2; Tbr2
• Vector: pCMV6-Entry
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210179 representing NM_010136
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTTGGGAGAGCAGCTCCTGGTGAGCTCGGTGAACCTGCCCGGCGCGCACTTCTACTCGCTGGAGA
  GTGCTCGCGGAGGAGGAGGAGGAGGAGGCGGAGGAGGAGGGGGAGGCGGAGGGAGCGTCAGCCTCCTCCC
  CGGTGCTGCCCCCTCGCCCCAGAGGCTGGACTTAGACAAAGCGTCCAAGAAGTTTCCGGGCAGTCTCCCG
  TGCCAGGCGGGGAGCGCAGAACCCGCAGGCGCCGGCGCGGGGGCCCCCGCGGCCATGCTCAGTGACGCGG
  ACGCTGGGGACACCTTCGGGAGCACCTCGGCGGTGGCCAAGCCCGGGCCCCCGGACGGCCGCAAGGGCTC
  CCCGTGCGCGGAGGAGGAGCTGCCCTCCGCCGCCACCGCCGCGGCCACCGCGCGCTACTCCATGGACAGC
  CTGAGCTCCGAGCGCTACTACCTCCCGTCGCCGGGACCGCAGGGCTCCGAGCTCGCCGCGCCCTGCTCGC
  TCTTCCAGTACCCGGCGGCGGCCGGAGCAGCCCACGGACCCGTGTACCCCGCGTCCAATGGCGCGCGCTA
  CCCCTACGGCTCCATGCTGCCCCCCGGTGGATTCCCCGCCGCCGTGTGCCCGCCCGCGAGGGCGCAGTTC
  GGCCCCGCTGCGGGTTCGGGGAGCGGCGCTGGTAGCAGCGGCGGTGGTGCCGGCGGTCCTGGCGCCTATC
  CCTACGGCCAGGGTTCTCCGCTCTACGGGCCATACGCCGGAACCTCAGCGGCCGGGTCTTGTGGAGGATT
  GGGGGGCCTAGGGGTGCCTGGCTCCGGCTTCCGCGCCCACGTCTACCTGTGCAACCGGCCCCTATGGCTC
  AAATTCCACCGGCACCAAACTGAGATGATCATCACCAAACAGGGCAGGCGCATGTTTCCTTTCTTGAGCT
  TCAACATAAACGGACTCAACCCCACCGCCCACTACAATGTTTTCGTGGAAGTGGTTCTGGCCGACCCTAA
  CCACTGGCGCTTCCAGGGGGGCAAGTGGGTGACCTGCGGCAAAGCGGACAATAACATGCAGGGCAATAAG
  ATGTACGTTCACCCAGAATCTCCTAACACTGGCTCCCACTGGATGAGGCAGGAGATTTCCTTTGGGAAGT
  TAAAACTCACCAATAACAAAGGTGCAAACAACAACAACACACAGATGATAGTGTTGCAGTCTCTGCACAA
  ATACCAACCGAGGCTGCACATCGTGGAAGTGACAGAGGACGGTGTGGAGGACTTGAATGAACCTTCCAAG
  ACTCAGACCTTCACCTTCTCAGAGACACAGTTCATCGCTGTGACGGCCTACCAAAACACGGATATCACCC
  AGCTAAAGATCGACCATAACCCCTTCGCCAAAGGCTTCCGGGACAACTACGATTCCATGTACACGGCTTC
  AGAAAATGACAGGTTAACTCCATCTCCCACGGATTCCCCTAGATCCCATCAGATTGTCCCTGGAGGTCGG
  TACGGCGTTCAAAACTTCTTCCCGGAGCCCTTTGTCAACACTTTGCCTCAAGCCCGATATTATAACGGTG
  AGAGAACCGTGCCACAGACCAACGGCCTCCTCTCACCCCAACAGAGCGAAGAGGTGGCCAACCCTCCCCA
  GCGGTGGCTTGTCACGCCTGTCCAGCAACCTGTGACCAACAAGCTAGACATCGGTTCTTATGAATCTGAA
  TATACTTCCAGTACCTTGCTCCCATATGGTATTAAGTCCTTGCCCCTGCAGACATCCCATGCCCTGGGGT
  ATTACCCTGACCCGACCTTCCCTGCTATGGCAGGGTGGGGAGGCCGTGGCGCTTATCAGAGGAAGATGGC
  AGCTGGACTACCATGGACATCCAGAATGAGCCCACCTGTCTTCCCAGAAGATCAGCTTGCCAAGGAAAAA
  GTTAAAGAAGAGATTAGTTCCTCCTGGATAGAGACTCCCCCCTCCATCAAGTCTCTAGACTCCAGCGACT
  CCGGGGTGTACAACAGCGCTTGCAAGAGAAAGCGCCTGTCTCCCAGCACCCCCAGCAATGGAAACTCGCC
  CCCCATAAAGTGTGAGGACATTAACACTGAAGAGTACAGTAAAGACACCTCCAAAGGCATGGGGGCTTAT
  TATGCTTTTTACACAAGTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210179 representing NM_010136
  Red=Cloning site Green=Tags(s)
  
  MQLGEQLLVSSVNLPGAHFYSLESARGGGGGGGGGGGGGGGSVSLLPGAAPSPQRLDLDKASKKFPGSLP
  CQAGSAEPAGAGAGAPAAMLSDADAGDTFGSTSAVAKPGPPDGRKGSPCAEEELPSAATAAATARYSMDS
  LSSERYYLPSPGPQGSELAAPCSLFQYPAAAGAAHGPVYPASNGARYPYGSMLPPGGFPAAVCPPARAQF
  GPAAGSGSGAGSSGGGAGGPGAYPYGQGSPLYGPYAGTSAAGSCGGLGGLGVPGSGFRAHVYLCNRPLWL
  KFHRHQTEMIITKQGRRMFPFLSFNINGLNPTAHYNVFVEVVLADPNHWRFQGGKWVTCGKADNNMQGNK
  MYVHPESPNTGSHWMRQEISFGKLKLTNNKGANNNNTQMIVLQSLHKYQPRLHIVEVTEDGVEDLNEPSK
  TQTFTFSETQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDSMYTASENDRLTPSPTDSPRSHQIVPGGR
  YGVQNFFPEPFVNTLPQARYYNGERTVPQTNGLLSPQQSEEVANPPQRWLVTPVQQPVTNKLDIGSYESE
  YTSSTLLPYGIKSLPLQTSHALGYYPDPTFPAMAGWGGRGAYQRKMAAGLPWTSRMSPPVFPEDQLAKEK
  VKEEISSSWIETPPSIKSLDSSDSGVYNSACKRKRLSPSTPSNGNSPPIKCEDINTEEYSKDTSKGMGAY
  YAFYTSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• Tag: Myc-DDK
• ACCN: NM_010136
• ORF Size: 2121 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.

Reference Data

• RefSeq: NM_010136.2, NM_010136.3, NP_034266
• RefSeq Size: 3731
• RefSeq ORF: 2124
• Locus ID: 13813
• Cytogenetics: 9 70.21 cM|9 F3
• MW: 75.3 kDa