Nln (NM_029447) Mouse Tagged ORF Clone

CAT#: MR210154

Nln (Myc-DDK-tagged) - Mouse neurolysin (metallopeptidase M3 family) (Nln), nuclear gene encoding mitochondrial protein

Product Images

Circular map for MR210154

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nln
• Synonyms: 4930472G13Rik; C79345
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210154 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCACCCTGTGCCTTTCGGCTCTGCGAGGCCTCCACAGAGCTGGTGGTTCCAGGATTCGGCTCAGAA
  TGACGTTGGGGAGGGAGGCGGCATCTCCTCTTCAGGCCATGTCTTCCTACACCGCCGCAGGCAGGAATGT
  TCTCAGATGGGACCTTTCTCCAGAGCAGATCAGGACGAGGACAGAGGAGCTCATAGCACAGACCAAGCAG
  GTGTATGATACCGTGGGGACAATCAACCTGGAGGATGTAACTTACGAGAACTGTCTGCAGGTGCTGGCTG
  ACATAGAAGTGAAGTACATAGTGGAAAGGACCATGCTGGACTTCCCTCAGCACGTGTCGTCTGACAGAGA
  AGTGCGGGCTGCAAGCACAGAAGCGGACAAAAGGCTTTCTCGTTTTGATATTGAGATGAGCATGAGAGAA
  GATGTATTTCAGAGAATTGTTCATTTACAAGAAACGTGCGATTTGGAGAAGATAAAGCCTGAGGCCAGGC
  GATACTTGGAAAAATCAATTAAAATGGGGAAAAGGAATGGACTCCATCTTCCAGAACACGTAAAAAATGA
  AATCAAATCAATGAAGAAGAGAATGAGTGAGCTATGCATTGACTTCAACAAAAACCTCAATGAGGATGAC
  ACGTCCCTTGTATTTTCCAAGGCTGAACTTGGTGCTCTTCCCGACGACTTCATTGACAGTTTAGAAAAAA
  CAGATGAAGACAAGTACAAAGTTACCTTAAAGTATCCCCACTATTTTCCTGTCATGAAGAAATGCTGTGT
  CCCTGAAACTCGAAGGAAGATGGAAATGGCTTTTCATACCAGGTGCAAAGAGGAAAACACCATAATTCTG
  CAACAGCTCCTGCCGCTGCGAGCACAAGTGGCCAAACTGCTCGGCTACAACACCCATGCTGACTTTGTCC
  TTGAACTGAACACTGCAAAGAGCACAAGCCATGTGGCAACCTTTCTAGATGATTTAAGCCAGAAATTAAA
  ACCCTTGGGTGAGGCTGAACGGGAGTTTATTTTGAGCTTGAAGAAGAAGGAATGCGAAGAGCGAGGTTTT
  GCATACGACGGGAAGATCAACGCATGGGACCTCCATTACTACATGACCCAGACAGAGGAGCTCAAGTACT
  CTGTGGACCAGGAGTCCCTCAAGGAGTACTTTCCCATTGAGGTGGTCACTGAAGGCCTGCTAAGCATCTA
  CCAGGAGCTGTTGGGACTTTCATTTGAACAAGTGGCTGATGCCCATGTTTGGAATAAGAGTGTTTCACTT
  TACACCGTGAAGGATAAAGCTACTGGAGAAGTGCTGGGGCAGTTCTACCTGGACCTCTATCCAAGGGAAG
  GAAAATACAACCATGCGGCCTGCTTTGGTCTCCAGCCAGGCTGCCTTCTCCCTGATGGCAGTCGGATGAT
  GTCTGTGGCTGCCCTGGTGGTCAACTTCTCTCAGCCCATAGCAGGCCGGCCCTCTCTCCTGAGACACGAC
  GAAGTGCGAACTTACTTCCATGAGTTTGGTCACGTCATGCATCAGATCTGTGCACAGACTGACTTTGCAC
  GATTTAGTGGAACAAATGTGGAAACTGACTTTGTAGAGGTGCCATCACAAATGCTTGAAAACTGGGTGTG
  GGACATTGACTCCCTGCGAAAACTGTCAAAACATTATAGAGACGGACACCCTATCACAGACGAGCTGCTG
  GAGAAGCTTGTGGCTTCGAGACTGGTCAACACAGGTCTTCTGACGCTTCGCCAAATTGTTTTGAGCAAAG
  TTGACCAGTCCCTCCATACCAATGCGTCGCTGGATGCCGCGAGCGAATACGCTAAATACTGCACAGAAAT
  TTTGGGAGTTGCAGCTACGCCAGGCACAAATATGCCAGCTACCTTTGGACATTTGGCAGGAGGGTATGAC
  GGCCAGTATTATGGATATCTTTGGAGTGAAGTCTTTTCCATGGACATGTTTCACAGCTGTTTCAGAAAAG
  AAGGGATTATGAATCCAGAGGTTGGAATGAAATACAGAAACCTAATCCTGAAGCCTGGGGGGTCCCTGGA
  CGGCATGGACATGCTCCAGAATTTCTTGCAACGTGAGCCAAACCAGAAAGCATTCCTGATGAGTCGAGGC
  CTGAATGCTTCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210154 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MITLCLSALRGLHRAGGSRIRLRMTLGREAASPLQAMSSYTAAGRNVLRWDLSPEQIRTRTEELIAQTKQ
  VYDTVGTINLEDVTYENCLQVLADIEVKYIVERTMLDFPQHVSSDREVRAASTEADKRLSRFDIEMSMRE
  DVFQRIVHLQETCDLEKIKPEARRYLEKSIKMGKRNGLHLPEHVKNEIKSMKKRMSELCIDFNKNLNEDD
  TSLVFSKAELGALPDDFIDSLEKTDEDKYKVTLKYPHYFPVMKKCCVPETRRKMEMAFHTRCKEENTIIL
  QQLLPLRAQVAKLLGYNTHADFVLELNTAKSTSHVATFLDDLSQKLKPLGEAEREFILSLKKKECEERGF
  AYDGKINAWDLHYYMTQTEELKYSVDQESLKEYFPIEVVTEGLLSIYQELLGLSFEQVADAHVWNKSVSL
  YTVKDKATGEVLGQFYLDLYPREGKYNHAACFGLQPGCLLPDGSRMMSVAALVVNFSQPIAGRPSLLRHD
  EVRTYFHEFGHVMHQICAQTDFARFSGTNVETDFVEVPSQMLENWVWDIDSLRKLSKHYRDGHPITDELL
  EKLVASRLVNTGLLTLRQIVLSKVDQSLHTNASLDAASEYAKYCTEILGVAATPGTNMPATFGHLAGGYD
  GQYYGYLWSEVFSMDMFHSCFRKEGIMNPEVGMKYRNLILKPGGSLDGMDMLQNFLQREPNQKAFLMSRG
  LNAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029447
• ORF Size: 2115 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029447.2
• RefSeq Size: 3780 bp
• RefSeq ORF: 2115 bp
• Locus ID: 75805
• UniProt ID: Q91YP2
• Cytogenetics: 13 D1
• MW: 80.4 kDa
• Gene Summary: Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.[UniProtKB/Swiss-Prot Function]